دورية أكاديمية
Three dimensional reconstruction of skin with melanoma: A model for study of invasion in vitro.
| العنوان: | Three dimensional reconstruction of skin with melanoma: A model for study of invasion in vitro. |
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| المؤلفون: | Aranha ESP; Faculty of Pharmaceutical Sciences, Post Graduate Program in Biodiversity and Biotechnology of the Amazon (Bionorte), Federal University of Amazonas, Manaus, Amazonas, Brazil., Mendonça LS; Faculty of Pharmaceutical Sciences, Federal University of Amazonas, Manaus, Amazonas, Brazil., Almeida BL; Faculty of Pharmaceutical Sciences, Federal University of Amazonas, Manaus, Amazonas, Brazil., da Silva EL; Drug Research and Development Center (NPDM), Federal University of Ceará, Fortaleza, Ceará, Brazil., Mesquita FP; Drug Research and Development Center (NPDM), Federal University of Ceará, Fortaleza, Ceará, Brazil., Lima ES; Faculty of Pharmaceutical Sciences, Federal University of Amazonas, Manaus, Amazonas, Brazil., Alves APNN; Drug Research and Development Center (NPDM), Federal University of Ceará, Fortaleza, Ceará, Brazil., de Moraes MEA; Drug Research and Development Center (NPDM), Federal University of Ceará, Fortaleza, Ceará, Brazil., Montenegro RC; Drug Research and Development Center (NPDM), Federal University of Ceará, Fortaleza, Ceará, Brazil., de Vasconcellos MC; Faculty of Pharmaceutical Sciences, Federal University of Amazonas, Manaus, Amazonas, Brazil. Electronic address: marnevasconcellos@yahoo.com.br. |
| المصدر: | Toxicology in vitro : an international journal published in association with BIBRA [Toxicol In Vitro] 2024 Aug; Vol. 99, pp. 105883. Date of Electronic Publication: 2024 Jun 25. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Pergamon Press Country of Publication: England NLM ID: 8712158 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-3177 (Electronic) Linking ISSN: 08872333 NLM ISO Abbreviation: Toxicol In Vitro Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Oxford ; New York : Pergamon Press, c1987- |
| مواضيع طبية MeSH: | Melanoma*/pathology , Melanoma*/metabolism , Matrix Metalloproteinase 2*/metabolism , Matrix Metalloproteinase 2*/genetics , Matrix Metalloproteinase 9*/metabolism , Matrix Metalloproteinase 9*/genetics , Skin Neoplasms*/pathology , Fibroblasts*/metabolism , Fibroblasts*/drug effects, Humans ; Cell Line, Tumor ; Skin/metabolism ; Skin/pathology ; Neoplasm Invasiveness ; Keratinocytes/drug effects ; Cell Line ; Vimentin/metabolism ; Vimentin/genetics |
| مستخلص: | Melanoma is a type of tumor skin with high metastatic potential. Reconstructed human skin, development for pre-clinic assay, are make using primary human cells, but with same limitations. The aim this study was to characterize a cell culture model, with structure similar to human skin containing melanoma cells entirely from cell lines. Reconstructed skin with melanoma were development using human fibroblasts (MRC5), human epidermal keratinocytes (HaCat), and human melanoma (SK-MEL-28) embedded in collagen type I. The structure was characterized by hematoxylin-eosin stained, as well as points of melanoma cell invasion, which was associated with activity of MMPs (MMP-2 and MMP-9) by zymographic method. Then, the gene expression of the target molecular mechanisms involved in melanoma progression were evaluated. Here, the model development showed a region epidermis organized and separated from the dermis, with fibroblast cells confined and melanoma cells form delimited area invasion. MMP-2 and MMP-9 were identified during of cell culture and gene expression of BRAF, NRAS, and Vimentin was confirmed. The proposed model provides one more opportunity to study in vitro tumor biology of melanoma and also to allows the study of new drugs with more reliable results then whats we would find in vivo. Competing Interests: Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Marne Carvalho de Vasconcellos has patent #BR 102019 017285 1 pending to Licensse. If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2023. Published by Elsevier Ltd.) |
| فهرسة مساهمة: | Keywords: Artificial skin; Melanoma; Organotipic culture; Three-dimensional culture |
| المشرفين على المادة: | EC 3.4.24.24 (Matrix Metalloproteinase 2) EC 3.4.24.35 (Matrix Metalloproteinase 9) EC 3.4.24.35 (MMP9 protein, human) EC 3.4.24.24 (MMP2 protein, human) 0 (Vimentin) |
| تواريخ الأحداث: | Date Created: 20240627 Date Completed: 20240721 Latest Revision: 20240721 |
| رمز التحديث: | 20240722 |
| DOI: | 10.1016/j.tiv.2024.105883 |
| PMID: | 38936442 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1879-3177 |
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| DOI: | 10.1016/j.tiv.2024.105883 |