دورية أكاديمية
NR4A1-mediated regulation of lipid droplets in progesterone synthesis in goat luteal cells†.
| العنوان: | NR4A1-mediated regulation of lipid droplets in progesterone synthesis in goat luteal cells†. |
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| المؤلفون: | Yu H; Animal Reproduction Laboratory, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China., Li X; Animal Reproduction Laboratory, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China., Zhao J; Animal Reproduction Laboratory, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China., Wang W; Animal Reproduction Laboratory, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China., Wei Q; Animal Reproduction Laboratory, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China., Mao D; Animal Reproduction Laboratory, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China. |
| المصدر: | Biology of reproduction [Biol Reprod] 2024 Sep 14; Vol. 111 (3), pp. 640-654. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Oxford University Press Country of Publication: United States NLM ID: 0207224 Publication Model: Print Cited Medium: Internet ISSN: 1529-7268 (Electronic) Linking ISSN: 00063363 NLM ISO Abbreviation: Biol Reprod Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2017- : New York : Oxford University Press Original Publication: Champaign, Ill. : Society for the Study of Reproduction |
| مواضيع طبية MeSH: | Goats* , Progesterone*/metabolism , Progesterone*/biosynthesis , Luteal Cells*/metabolism , Luteal Cells*/drug effects , Nuclear Receptor Subfamily 4, Group A, Member 1*/metabolism , Nuclear Receptor Subfamily 4, Group A, Member 1*/genetics , Lipid Droplets*/metabolism, Animals ; Female ; Lipid Metabolism/physiology ; Cells, Cultured |
| مستخلص: | Nuclear receptor NR4A1 is a key factor in glycolipid metabolism and steroidogenesis, while lipid droplets serve as crucial dynamic organelles for lipid metabolism in luteal cells. To investigate the effects of NR4A1 on lipid droplet metabolism and progesterone (P4) synthesis in goat corpus luteum in vitro, luteal cells from the middle-cyclic corpus luteum were isolated and treated with Cytosporone B (CSNB, an agonist) or siRNA of NR4A1. Results showed that both low (1 μM) and high (50 μM) concentrations of CSNB promoted lipid droplet accumulation, while NR4A1 knockdown reduced lipid droplet content. CSNB increased while siNR4A1 decreased total cholesterol content; however, CSNB and siNR4A1 did not change triglyceride content. CSNB increased the expression of perilipins at mRNA and protein levels, also increased LDLR, SCARB1, SREBFs, and HMGCR mRNA abundance. Treatment with siNR4A1 revealed opposite results of CSNB, except for HMCGR and SREBF2. For steroidogenesis, 1 μM CSNB increased, but 50 μM CSNB inhibited P4 synthesis, NR4A1 knockdown also reduced the P4 level. Further analysis demonstrated that 1 μM CSNB increased the protein levels of StAR, HSD3B, and P-HSL, while 50 μM CSNB decreased StAR, HSD3B, and CYP11A1 protein levels. Moreover, 50 μM CSNB impaired active mitochondria, reduced the BCL2, and increased DRP1, Caspase 3, and cleaved-Caspase 3 protein levels. siNR4A1 consistently downregulated the P-HSL/HSL ratio and the steroidogenic protein levels. In conclusion, NR4A1-mediated lipid droplets are involved in the regulation of progesterone synthesis in goat luteal cells. (© The Author(s) 2024. Published by Oxford University Press on behalf of Society for the Study of Reproduction. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.) |
| معلومات مُعتمدة: | 31972565 National Natural Science Foundation of China |
| فهرسة مساهمة: | Keywords: NR4A1; goat; lipid droplet; luteal cell; progesterone |
| المشرفين على المادة: | 4G7DS2Q64Y (Progesterone) 0 (Nuclear Receptor Subfamily 4, Group A, Member 1) |
| تواريخ الأحداث: | Date Created: 20240627 Date Completed: 20240915 Latest Revision: 20240915 |
| رمز التحديث: | 20240916 |
| DOI: | 10.1093/biolre/ioae085 |
| PMID: | 38936833 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1529-7268 |
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| DOI: | 10.1093/biolre/ioae085 |