دورية أكاديمية
Cold Physical Plasma Reduces Motility of Various Bone Sarcoma Cells While Remodeling the Cytoskeleton.
| العنوان: | Cold Physical Plasma Reduces Motility of Various Bone Sarcoma Cells While Remodeling the Cytoskeleton. |
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| المؤلفون: | Nitsch A; Center for Orthopedics, Trauma Surgery and Rehabilitation Medicine, University Medicine Greifswald, Greifswald, Germany; andreas.nitsch@med.uni-greifswald.de., Marthaler P; Center for Orthopedics, Trauma Surgery and Rehabilitation Medicine, University Medicine Greifswald, Greifswald, Germany., Qarqash S; Center for Orthopedics, Trauma Surgery and Rehabilitation Medicine, University Medicine Greifswald, Greifswald, Germany., Bemmann M; Center for Orthopedics, Trauma Surgery and Rehabilitation Medicine, University Medicine Greifswald, Greifswald, Germany., Bekeschus S; ZIK Plasmatis, Leibniz Institute for Plasma Science and Technology (INP), Greifswald, Germany.; Clinic and Policlinic for Dermatology and Venerology, Rostock University Medical Center, Rostock, Germany., Wassilew GI; Center for Orthopedics, Trauma Surgery and Rehabilitation Medicine, University Medicine Greifswald, Greifswald, Germany., Haralambiev L; Center for Orthopedics, Trauma Surgery and Rehabilitation Medicine, University Medicine Greifswald, Greifswald, Germany. |
| المصدر: | In vivo (Athens, Greece) [In Vivo] 2024 Jul-Aug; Vol. 38 (4), pp. 1571-1578. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: International Institute of Anticancer Research Country of Publication: Greece NLM ID: 8806809 Publication Model: Print Cited Medium: Internet ISSN: 1791-7549 (Electronic) Linking ISSN: 0258851X NLM ISO Abbreviation: In Vivo Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: <2000- > : Attiki, Greece : International Institute of Anticancer Research Original Publication: Athens : Dr. J.G. Delinassios |
| مواضيع طبية MeSH: | Cell Movement*/drug effects , Plasma Gases*/pharmacology , Bone Neoplasms*/pathology , Bone Neoplasms*/metabolism , Cell Survival*/drug effects , Cell Proliferation*/drug effects , Cytoskeleton*/metabolism , Actin Cytoskeleton*/metabolism , Actin Cytoskeleton*/drug effects, Humans ; Cell Line, Tumor ; Osteosarcoma/pathology ; Osteosarcoma/metabolism ; Actins/metabolism ; Sarcoma/pathology ; Sarcoma/metabolism |
| مستخلص: | Background/aim: Cold physical plasma (CPP) has emerged as an effective therapy in oncology by inducing cytotoxic effects in various cancer cells, including chondrosarcoma (CS), Ewing's sarcoma (ES), and osteosarcoma (OS). The current study investigated the impact of CPP on cell motility in CS (CAL-78), ES (A673), and OS (U2-OS) cell lines, focusing on the actin cytoskeleton. Materials and Methods: The CASY Cell Counter and Analyzer was used to study cell proliferation and determine the optimal concentrations of fetal calf serum to maintain viability without stimulation of cell proliferation. CellTiter-BlueCell viability assay was used to determine the effects of CPP on the viability of bone sarcoma cells. The Radius assay was used to determine cell migration. Staining for Deoxyribonuclease I, G-actin, and F-actin was used to assay for the effects on the cytoskeleton. Results: Reductions in cell viability and motility were observed across all cell lines following CPP treatment. CPP induced changes in the actin cytoskeleton, leading to decreased cell motility. Conclusion: CPP effectively reduces the motility of bone sarcoma cells by altering the actin cytoskeleton. These findings underscore CPP's potential as a therapeutic tool for bone sarcomas and highlight the need for further research in this area. (Copyright © 2024, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.) |
| References: | Cancers (Basel). 2023 Jan 12;15(2):. (PMID: 36672432) Arch Biochem Biophys. 2023 Oct 1;747:109757. (PMID: 37742933) Biomedicines. 2021 Oct 26;9(11):. (PMID: 34829774) Biochim Biophys Acta. 2007 May;1773(5):642-52. (PMID: 16926057) J Biophotonics. 2019 Jan;12(1):e201800046. (PMID: 29931745) PLoS One. 2015 Mar 24;10(3):e0119111. (PMID: 25803024) Sci Rep. 2020 Jul 16;10(1):11788. (PMID: 32678153) Oxid Med Cell Longev. 2021 Dec 24;2021:9916796. (PMID: 35284036) Cancers (Basel). 2020 Jan 22;12(2):. (PMID: 31979114) PLoS One. 2014 May 30;9(5):e98652. (PMID: 24878760) Cell Physiol Biochem. 2019;52(1):119-140. (PMID: 30790509) Ann Biomed Eng. 2010 Mar;38(3):748-57. (PMID: 20013154) Int J Mol Sci. 2023 Apr 12;24(8):. (PMID: 37108320) Sci Rep. 2019 Jan 24;9(1):634. (PMID: 30679720) J Clin Med. 2021 Feb 23;10(4):. (PMID: 33672274) Sci Rep. 2012;2:636. (PMID: 22957140) Biomedicines. 2022 Mar 17;10(3):. (PMID: 35327489) Exp Dermatol. 2017 Feb;26(2):156-162. (PMID: 27492871) Anticancer Res. 2016 Nov;36(11):5915-5922. (PMID: 27793916) Anticancer Res. 2017 Dec;37(12):6739-6744. (PMID: 29187451) Int J Mol Sci. 2020 Jun 23;21(12):. (PMID: 32585948) Biochem Biophys Res Commun. 2016 May 13;473(4):1125-1132. (PMID: 27067049) PLoS One. 2013 Nov 12;8(11):e79325. (PMID: 24265766) Int J Mol Sci. 2017 Sep 19;18(9):. (PMID: 28925941) PLoS One. 2013 Sep 11;8(9):e73741. (PMID: 24040051) Int J Mol Sci. 2020 Mar 26;21(7):. (PMID: 32225067) Int J Mol Sci. 2021 Apr 08;22(8):. (PMID: 33917790) Cell. 2003 Feb 21;112(4):453-65. (PMID: 12600310) Anticancer Res. 2020 Nov;40(11):6205-6212. (PMID: 33109557) Biomedicines. 2023 Feb 17;11(2):. (PMID: 36831137) PLoS One. 2014 Mar 25;9(3):e92198. (PMID: 24667444) J Biotechnol. 2010 Dec;150(4):530-8. (PMID: 20959125) Cancers (Basel). 2020 Apr 23;12(4):. (PMID: 32340164) Biosens Bioelectron. 2011 Oct 15;28(1):333-8. (PMID: 21820891) Oncotarget. 2017 Feb 28;8(9):15977-15995. (PMID: 27845910) J R Soc Interface. 2012 Jan 7;9(66):147-57. (PMID: 21653568) |
| فهرسة مساهمة: | Keywords: CAP; CPP; Ewing’s sarcoma; NIPP; cell motility; chondrosarcoma; cold atmospheric pressure plasma; medical gas plasma; osteosarcoma |
| المشرفين على المادة: | 0 (Plasma Gases) 0 (Actins) |
| تواريخ الأحداث: | Date Created: 20240627 Date Completed: 20240627 Latest Revision: 20240704 |
| رمز التحديث: | 20240704 |
| مُعرف محوري في PubMed: | PMC11215588 |
| DOI: | 10.21873/invivo.13607 |
| PMID: | 38936915 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1791-7549 |
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| DOI: | 10.21873/invivo.13607 |