دورية أكاديمية

Rapid improvement of grain appearance in three-line hybrid rice via CRISPR/Cas9 editing of grain size genes.

التفاصيل البيبلوغرافية
العنوان: Rapid improvement of grain appearance in three-line hybrid rice via CRISPR/Cas9 editing of grain size genes.
المؤلفون: Huang J; Rice Research Institute, Guangxi Academy of Agricultural Sciences/Guangxi Key Laboratory of Rice Genetics and Breeding, Nanning, 530007, People's Republic of China., Chen W; Rice Research Institute, Guangxi Academy of Agricultural Sciences/Guangxi Key Laboratory of Rice Genetics and Breeding, Nanning, 530007, People's Republic of China., Gao L; Guangxi Academy of Agricultural Sciences/Guangxi Crop Genetic Improvement and Biotechnology Laboratory, Nanning, 530007, People's Republic of China., Qing D; Rice Research Institute, Guangxi Academy of Agricultural Sciences/Guangxi Key Laboratory of Rice Genetics and Breeding, Nanning, 530007, People's Republic of China., Pan Y; Rice Research Institute, Guangxi Academy of Agricultural Sciences/Guangxi Key Laboratory of Rice Genetics and Breeding, Nanning, 530007, People's Republic of China., Zhou W; Rice Research Institute, Guangxi Academy of Agricultural Sciences/Guangxi Key Laboratory of Rice Genetics and Breeding, Nanning, 530007, People's Republic of China., Wu H; Guangxi Academy of Agricultural Sciences/Guangxi Crop Genetic Improvement and Biotechnology Laboratory, Nanning, 530007, People's Republic of China., Li J; Rice Research Institute, Guangxi Academy of Agricultural Sciences/Guangxi Key Laboratory of Rice Genetics and Breeding, Nanning, 530007, People's Republic of China., Ma C; Guangxi Academy of Agricultural Sciences/Guangxi Crop Genetic Improvement and Biotechnology Laboratory, Nanning, 530007, People's Republic of China., Zhu C; Key Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education, Jiangxi Agricultural University, Nanchang, 330045, People's Republic of China. zhuchanglan@163.com., Dai G; Rice Research Institute, Guangxi Academy of Agricultural Sciences/Guangxi Key Laboratory of Rice Genetics and Breeding, Nanning, 530007, People's Republic of China. 25266220@qq.com., Deng G; Rice Research Institute, Guangxi Academy of Agricultural Sciences/Guangxi Key Laboratory of Rice Genetics and Breeding, Nanning, 530007, People's Republic of China. dengguofu163@163.com.
المصدر: TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik [Theor Appl Genet] 2024 Jun 27; Vol. 137 (7), pp. 173. Date of Electronic Publication: 2024 Jun 27.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Springer Country of Publication: Germany NLM ID: 0145600 Publication Model: Electronic Cited Medium: Internet ISSN: 1432-2242 (Electronic) Linking ISSN: 00405752 NLM ISO Abbreviation: Theor Appl Genet Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Berlin, New York, Springer
مواضيع طبية MeSH: Oryza*/genetics , Oryza*/growth & development , Gene Editing*/methods , CRISPR-Cas Systems* , Edible Grain*/genetics , Edible Grain*/growth & development, Genes, Plant ; Phenotype ; Plant Breeding/methods ; Mutation ; Plants, Genetically Modified/genetics ; Plants, Genetically Modified/growth & development ; Seeds/genetics ; Seeds/growth & development
مستخلص: Key Message: Genetic editing of grain size genes quickly improves three-line hybrid rice parents to increase the appearance quality and yield of hybrid rice. Grain size affects rice yield and quality. In this study, we used CRISPR/Cas9 to edit the grain size gene GW8 in the maintainer line WaitaiB (WTB) and restorer line Guanghui998 (GH998). The new slender sterile line WTEA (gw8) was obtained in the BC 2 F 1 generation by transferring the grain mutation of the maintainer plant to the corresponding sterile line WantaiA (WTA, GW8) in the T 1 generation. Two slender restorer lines, GH998E1 (gw8(II)) and GH998E2 (gw8(I)), were obtained in T 1 generation. In the early stage, new sterile and restorer lines in grain mutations were created by targeted editing of GS3, TGW3, and GW8 genes. These parental lines were mated to detect the impact of grain-type mutations on hybrid rice yield and quality. Mutations in gs3, gw8, and tgw3 had a minimal impact on agronomic traits except the grain size and thousand-grain weight. The decrease in grain width in the combination mainly came from gw8/gw8, gs3/gs3 increased the grain length, gs3/gs3-gw8/gw8 had a more significant effect on the grain length, and gs3/gs3-gw8/gw8(I) contributed more to grain length than gs3/gs3-gw8/gw8(II). The heterozygous TGW3/tgw3 may not significantly increase grain length. Electron microscopy revealed that the low-chalky slender-grain variety had a cylindrical grain shape, a uniform distribution of endosperm cells, and tightly arranged starch grains. Quantitative fluorescence analysis of endospermdevelopment-related genes showed that the combination of slender grain hybrid rice caused by gs3 and gw8 mutations promoted endosperm development and improved appearance quality. An appropriate grain size mutation resulted in hybrid rice varieties with high yield and quality.
(© 2024. The Author(s).)
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معلومات مُعتمدة: U20A2032 National Natural Science Foundation of China; NSFC32360508 National Natural Science Foundation of China; NSFC32060454 National Natural Science Foundation of China; NSFC32160645 National Natural Science Foundation of China
تواريخ الأحداث: Date Created: 20240627 Date Completed: 20240627 Latest Revision: 20240715
رمز التحديث: 20240715
مُعرف محوري في PubMed: PMC11211133
DOI: 10.1007/s00122-024-04627-8
PMID: 38937300
قاعدة البيانات: MEDLINE
الوصف
تدمد:1432-2242
DOI:10.1007/s00122-024-04627-8