دورية أكاديمية
أعرض في EDS

Association of CUL4B with the pathogenesis of age-related cataract.

التفاصيل البيبلوغرافية
العنوان: Association of CUL4B with the pathogenesis of age-related cataract.
المؤلفون: Karaosmanoğlu C; Department of Medical Genetics, Faculty of Medicine, Afyonkarahisar Health Sciences University, Afyonkarahisar, Turkey. cemkaraosmanoglu@gmail.com., Yıldız SH; Department of Medical Genetics, Faculty of Medicine, Afyonkarahisar Health Sciences University, Afyonkarahisar, Turkey., Akdoğan M; Department of Ophthalmology, Faculty of Medicine, Afyonkarahisar Health Sciences University, Afyonkarahisar, Turkey., Erdoğan MÖ; Department of Medical Genetics, Faculty of Medicine, Afyonkarahisar Health Sciences University, Afyonkarahisar, Turkey.
المصدر: International ophthalmology [Int Ophthalmol] 2024 Jun 27; Vol. 44 (1), pp. 288. Date of Electronic Publication: 2024 Jun 27.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Kluwer Country of Publication: Netherlands NLM ID: 7904294 Publication Model: Electronic Cited Medium: Internet ISSN: 1573-2630 (Electronic) Linking ISSN: 01655701 NLM ISO Abbreviation: Int Ophthalmol Subsets: MEDLINE
أسماء مطبوعة: Publication: Dordrecht : Kluwer
Original Publication: The Hague, Junk.
مواضيع طبية MeSH: Cataract*/metabolism , Cataract*/genetics , Cataract*/etiology , Cullin Proteins*/genetics , Cullin Proteins*/metabolism , Cullin Proteins*/biosynthesis , Apoptosis*, Humans ; Male ; Female ; Aged ; Blotting, Western ; Real-Time Polymerase Chain Reaction ; Middle Aged ; Aging ; Gene Expression Regulation ; Lens Capsule, Crystalline/metabolism ; Lens Capsule, Crystalline/pathology ; In Situ Nick-End Labeling
مستخلص: Purpose: Age-related cataract (ARC) is the most common cause of visual impairment and blindness in older adults. However, the role of CUL4B in the ARC remains unclear. Therefore, we investigated CUL4B expression and its effects on apoptosis.
Materials and Methods: CUL4B expression levels were detected by a quantitative real-time polymerase chain reaction from the anterior lens capsules of patients with ARC and HLE-B3 cells treated with different concentrations of H 2 O 2 . CUL4B expression was silenced by siRNA transfection to evaluate apoptosis. CUL4B and apoptotic proteins B cell lymphoma 2 (Bcl-2), myeloid cell leukemia 1 (Mcl-1), caspase-3, cleaved caspase-3, Bax, Bak, and Bid were assessed using western blot analysis. Apoptosis was monitored using the TUNEL assay.
Results: CUL4B expression was downregulated in the anterior lens capsules (P < 0.0001) and H 2 O 2 -treated HLE-B3 cells (P = 0.0405). CUL4B protein levels were significantly lower in 100 µmol/L (P = 0.0012) and 200 µmol/L (P = 0.0041) H 2 O 2 -treated HLE-B3 cells than in the untreated cells. CUL4B expression was significantly knocked down at the mRNA (P = 0.0043) and protein levels (P = 0.0002) in HLE-B3 cells. Bcl-2 (P = 0.0199), Mcl-1 (P = 0.0042), and caspase-3 (P = 0.0142) were significantly downregulated, whereas cleaved caspase-3 (P = 0.0089) and Bak (P = 0.009) were significantly upregulated in the knockdown group. The TUNEL assay showed a greater induction of apoptosis.
Conclusions: CUL4B downregulation promotes the apoptosis of lens epithelial cells. Our study may help in understanding the role of CUL4B in ARC pathogenesis.
(© 2024. The Author(s), under exclusive licence to Springer Nature B.V.)
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فهرسة مساهمة: Keywords: Age-related cataract; Apoptosis; Cullin-4B; Lens epithelial cells; Pathogenesis
المشرفين على المادة: 0 (Cullin Proteins)
0 (CUL4B protein, human)
تواريخ الأحداث: Date Created: 20240627 Date Completed: 20240627 Latest Revision: 20240719
رمز التحديث: 20240719
DOI: 10.1007/s10792-024-03011-1
PMID: 38937308
قاعدة البيانات: MEDLINE
الوصف
تدمد:1573-2630
DOI:10.1007/s10792-024-03011-1