دورية أكاديمية
أعرض في EDS

Distinct functions of microtubules and actin filaments in the transportation of the male germ unit in pollen.

التفاصيل البيبلوغرافية
العنوان: Distinct functions of microtubules and actin filaments in the transportation of the male germ unit in pollen.
المؤلفون: Wang X; Key Laboratory of Cell Proliferation and Regulation Biology of Ministry of Education, College of Life Sciences, Beijing Normal University, 100875, Beijing, China., Li T; Key Laboratory of Cell Proliferation and Regulation Biology of Ministry of Education, College of Life Sciences, Beijing Normal University, 100875, Beijing, China., Xu J; Key Laboratory of Cell Proliferation and Regulation Biology of Ministry of Education, College of Life Sciences, Beijing Normal University, 100875, Beijing, China., Zhang F; Key Laboratory of Cell Proliferation and Regulation Biology of Ministry of Education, College of Life Sciences, Beijing Normal University, 100875, Beijing, China., Liu L; Key Laboratory of Cell Proliferation and Regulation Biology of Ministry of Education, College of Life Sciences, Beijing Normal University, 100875, Beijing, China., Wang T; Key Laboratory of Cell Proliferation and Regulation Biology of Ministry of Education, College of Life Sciences, Beijing Normal University, 100875, Beijing, China., Wang C; Key Laboratory of Cell Proliferation and Regulation Biology of Ministry of Education, College of Life Sciences, Beijing Normal University, 100875, Beijing, China., Ren H; Key Laboratory of Cell Proliferation and Regulation Biology of Ministry of Education, College of Life Sciences, Beijing Normal University, 100875, Beijing, China. hren@bnu.edu.cn.; Center for Biological Science and Technology, Guangdong Zhuhai-Macao Joint Biotech Laboratory, Beijing Normal University, 519087, Zhuhai, China. hren@bnu.edu.cn., Zhang Y; Key Laboratory of Cell Proliferation and Regulation Biology of Ministry of Education, College of Life Sciences, Beijing Normal University, 100875, Beijing, China. yi.zhang@bnu.edu.cn.
المصدر: Nature communications [Nat Commun] 2024 Jun 27; Vol. 15 (1), pp. 5448. Date of Electronic Publication: 2024 Jun 27.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Nature Pub. Group Country of Publication: England NLM ID: 101528555 Publication Model: Electronic Cited Medium: Internet ISSN: 2041-1723 (Electronic) Linking ISSN: 20411723 NLM ISO Abbreviation: Nat Commun Subsets: MEDLINE
أسماء مطبوعة: Original Publication: [London] : Nature Pub. Group
مواضيع طبية MeSH: Microtubules*/metabolism , Actin Cytoskeleton*/metabolism , Kinesins*/metabolism , Pollen*/metabolism , Actomyosin*/metabolism, Pollen Tube/metabolism ; Pollen Tube/growth & development ; Cell Nucleus/metabolism ; Arabidopsis/metabolism ; Cytoplasmic Streaming ; Germination/physiology
مستخلص: Flowering plants rely on the polarized growth of pollen tubes to deliver sperm cells (SCs) to the embryo sac for double fertilization. In pollen, the vegetative nucleus (VN) and two SCs form the male germ unit (MGU). However, the mechanism underlying directional transportation of MGU is not well understood. In this study, we provide the first full picture of the dynamic interplay among microtubules, actin filaments, and MGU during pollen germination and tube growth. Depolymerization of microtubules and inhibition of kinesin activity result in an increased velocity and magnified amplitude of VN's forward and backward movement. Pharmacological washout experiments further suggest that microtubules participate in coordinating the directional movement of MGU. In contrast, suppression of the actomyosin system leads to a reduced velocity of VN mobility but without a moving pattern change. Moreover, detailed observation shows that the direction and velocity of VN's movement are in close correlations with those of the actomyosin-driven cytoplasmic streaming surrounding VN. Therefore, we propose that while actomyosin-based cytoplasmic streaming influences on the oscillational movement of MGU, microtubules and kinesins avoid MGU drifting with the cytoplasmic streaming and act as the major regulator for fine-tuning the proper positioning and directional migration of MGU in pollen.
(© 2024. The Author(s).)
References: Curr Biol. 2016 Feb 8;26(3):R125-39. (PMID: 26859271)
Curr Opin Plant Biol. 2015 Dec;28:111-9. (PMID: 26550939)
Proc Natl Acad Sci U S A. 2014 Aug 12;111(32):11900-5. (PMID: 25074908)
J Cell Biol. 2017 Jun 5;216(6):1705-1714. (PMID: 28442535)
Plant Cell. 2021 May 31;33(4):1252-1267. (PMID: 33638636)
New Phytol. 2019 Apr;222(2):687-693. (PMID: 30556141)
Curr Opin Plant Biol. 2013 Dec;16(6):710-7. (PMID: 24446546)
Front Cell Dev Biol. 2020 Dec 03;8:614821. (PMID: 33344460)
Plant Cell Physiol. 2007 Feb;48(2):345-61. (PMID: 17204488)
J Exp Bot. 2010 Jul;61(12):3423-37. (PMID: 20566563)
Annu Rev Cell Dev Biol. 2010;26:421-44. (PMID: 20507227)
Annu Rev Plant Biol. 2013;64:243-65. (PMID: 23451782)
Annu Rev Plant Biol. 2008;59:547-72. (PMID: 18444907)
Front Plant Sci. 2015 Apr 21;6:246. (PMID: 25954283)
ACS Chem Biol. 2015 Feb 20;10(2):554-60. (PMID: 25402598)
Protoplasma. 2010 Dec;247(3-4):131-43. (PMID: 20922548)
Plant Physiol. 2015 Nov;169(3):1946-60. (PMID: 26358416)
Development. 2009 Aug;136(16):2725-33. (PMID: 19605495)
BMC Genomics. 2006 Jan 31;7:18. (PMID: 16448571)
Semin Cell Dev Biol. 2023 Mar 15;137:26-37. (PMID: 35067438)
Nat Protoc. 2014 Feb;9(2):457-63. (PMID: 24481272)
Angew Chem Int Ed Engl. 2009;48(48):9072-6. (PMID: 19856362)
Protoplasma. 2007;230(3-4):171-82. (PMID: 17458632)
Mol Gen Genet. 1989 Jun;217(2-3):240-5. (PMID: 2770694)
Annu Rev Cell Dev Biol. 2001;17:159-87. (PMID: 11687487)
Plant J. 2013 Nov;76(3):367-79. (PMID: 23906068)
Mol Plant. 2018 Nov 5;11(11):1389-1399. (PMID: 30296598)
Front Plant Sci. 2015 Feb 10;6:51. (PMID: 25713579)
J Exp Bot. 2011 Mar;62(5):1621-31. (PMID: 21357775)
Mol Plant. 2013 Jul;6(4):1109-30. (PMID: 23770840)
Plant Cell. 2010 Jun;22(6):1868-82. (PMID: 20525854)
Cell Rep. 2023 Nov 28;42(11):113319. (PMID: 37897722)
J Exp Bot. 2009;60(2):495-508. (PMID: 19112169)
Nat Plants. 2017 Jun 06;3:17079. (PMID: 28585562)
J Cell Biol. 2010 Oct 4;191(1):115-28. (PMID: 20921138)
Nat Commun. 2021 Apr 22;12(1):2331. (PMID: 33888710)
Development. 2018 Jan 8;145(1):. (PMID: 29217755)
Nat Struct Mol Biol. 2009 Jan;16(1):80-8. (PMID: 19122661)
Mol Plant. 2008 Jul;1(4):686-702. (PMID: 19825573)
Front Plant Sci. 2021 Feb 16;11:601282. (PMID: 33664751)
J Cell Sci. 1995 Jul;108 ( Pt 7):2549-63. (PMID: 7593296)
Curr Biol. 2013 Sep 23;23(18):1776-81. (PMID: 23973298)
Proc Natl Acad Sci U S A. 2009 Feb 17;106(7):2194-9. (PMID: 19164528)
Mol Plant. 2013 Jul;6(4):1053-64. (PMID: 23873928)
معلومات مُعتمدة: 91854206 National Natural Science Foundation of China (National Science Foundation of China); 32170335 National Natural Science Foundation of China (National Science Foundation of China); 32001522 National Natural Science Foundation of China (National Science Foundation of China); 32100297 National Natural Science Foundation of China (National Science Foundation of China); 32270350 National Natural Science Foundation of China (National Science Foundation of China); 32070194 National Natural Science Foundation of China (National Science Foundation of China)
المشرفين على المادة: EC 3.6.4.4 (Kinesins)
9013-26-7 (Actomyosin)
تواريخ الأحداث: Date Created: 20240627 Date Completed: 20240627 Latest Revision: 20240630
رمز التحديث: 20240630
مُعرف محوري في PubMed: PMC11211427
DOI: 10.1038/s41467-024-49323-9
PMID: 38937444
قاعدة البيانات: MEDLINE
الوصف
تدمد:2041-1723
DOI:10.1038/s41467-024-49323-9