دورية أكاديمية
Human NMO-IgG Induced Different Pathological and Immunological Changes in the CNS and Peripheral Tissues of Mice.
| العنوان: | Human NMO-IgG Induced Different Pathological and Immunological Changes in the CNS and Peripheral Tissues of Mice. |
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| المؤلفون: | Xiang W; Department of Neurology, Renji Hospital, Shanghai Jiao Tong University School of Medicine, 200127 Shanghai, China., Bai S; Department of Neurology, Renji Hospital, Shanghai Jiao Tong University School of Medicine, 200127 Shanghai, China., Wang K; Department of Neurology, Renji Hospital, Shanghai Jiao Tong University School of Medicine, 200127 Shanghai, China., Peng J; Department of Neurology, Renji Hospital, Shanghai Jiao Tong University School of Medicine, 200127 Shanghai, China., Wang Z; Department of Neurology, Renji Hospital, Shanghai Jiao Tong University School of Medicine, 200127 Shanghai, China., Han L; Department of Neurology, Renji Hospital, Shanghai Jiao Tong University School of Medicine, 200127 Shanghai, China., Xie C; Department of Neurology, Renji Hospital, Shanghai Jiao Tong University School of Medicine, 200127 Shanghai, China., Guan Y; Department of Neurology, Renji Hospital, Shanghai Jiao Tong University School of Medicine, 200127 Shanghai, China. |
| المصدر: | Journal of integrative neuroscience [J Integr Neurosci] 2024 Jun 20; Vol. 23 (6), pp. 119. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: IMR Press Country of Publication: Singapore NLM ID: 101156357 Publication Model: Print Cited Medium: Internet ISSN: 0219-6352 (Print) Linking ISSN: 02196352 NLM ISO Abbreviation: J Integr Neurosci Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2021-: Singapore : IMR Press Original Publication: London : Imperial College Press, c2002- |
| مواضيع طبية MeSH: | Neuromyelitis Optica*/immunology , Neuromyelitis Optica*/pathology , Mice, Inbred C57BL* , Immunoglobulin G* , Aquaporin 4*/immunology, Animals ; Female ; Humans ; Mice ; Disease Models, Animal ; Microglia/metabolism ; Microglia/immunology ; Microglia/drug effects ; Autoantibodies/immunology ; Astrocytes/immunology ; Astrocytes/metabolism ; Astrocytes/pathology ; Glial Fibrillary Acidic Protein/metabolism ; Glial Fibrillary Acidic Protein/immunology ; Central Nervous System/immunology ; Central Nervous System/metabolism ; Central Nervous System/pathology |
| مستخلص: | Objectives: The majority of neuromyelitis optica spectrum disorders (NMOSD) patients are seropositive for aquaporin-4 (AQP4)-specific antibodies [also named neuromyelitis optica immunoglobulin G antibodies (NMO-IgG)]. Although NMO-IgG can induce pathological changes in the central nervous system (CNS), the immunological changes in the CNS and peripheral tissue remain largely unknown. We investigated whether NMO-IgG binds to tissue expressing AQP4 and induces immunological changes in the peripheral tissue and CNS. Methods: C57BL/6 female mice were assigned into an NMOSD or control group. Pathological and immunological changes in peripheral tissue and CNS were measured by immunostaining and flow cytometry, respectively. Motor impairment was measured by open-field test. Results: We found that NMO-IgG did bind to astrocyte- and AQP4-expressing peripheral tissue, but induced glial fibrillary acidic protein and AQP4 loss only in the CNS. NMO-IgG induced the activation of microglia and modulated microglia polarization toward the classical (M1) phenotype, but did not affect innate or adaptive immune cells in the peripheral immune system, such as macrophages, neutrophils, Th17/Th1, or IL-10-producing B cells. In addition, NMOSD mice showed significantly less total distance traveled and higher immobility time in the open field. Conclusions: We found that injection of human NMO-IgG led to astrocytopathic lesions with microglial activation in the CNS. However, there were no significant pathological or immunological changes in the peripheral tissues. Competing Interests: The authors declare no conflict of interest. (© 2024 The Author(s). Published by IMR Press.) |
| معلومات مُعتمدة: | 81801195 National Natural Science Foundation of China; SHSMU-ZDCX20211901 Innovative research team of high-level local universities in Shanghai; Shanghai "Rising Stars of Medical Talents" Youth Development Program |
| فهرسة مساهمة: | Keywords: aquaporin-4; microglia; neuromyelitis optica spectrum disorders; pathological changes |
| المشرفين على المادة: | 0 (Immunoglobulin G) 0 (Aquaporin 4) 0 (Autoantibodies) 0 (Glial Fibrillary Acidic Protein) |
| تواريخ الأحداث: | Date Created: 20240628 Date Completed: 20240628 Latest Revision: 20240703 |
| رمز التحديث: | 20240703 |
| DOI: | 10.31083/j.jin2306119 |
| PMID: | 38940087 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 0219-6352 |
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| DOI: | 10.31083/j.jin2306119 |