دورية أكاديمية
PTEN and the PTEN-like phosphatase CnrN have both distinct and overlapping roles in a Dictyostelium chemorepulsion pathway.
| العنوان: | PTEN and the PTEN-like phosphatase CnrN have both distinct and overlapping roles in a Dictyostelium chemorepulsion pathway. |
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| المؤلفون: | Consalvo KM; Department of Biology, Texas A&M University, College Station, TX 77843-3474, USA., Rijal R; Department of Biology, Texas A&M University, College Station, TX 77843-3474, USA., Beruvides SL; Department of Biology, Texas A&M University, College Station, TX 77843-3474, USA., Mitchell R; Department of Biology, Texas A&M University, College Station, TX 77843-3474, USA., Beauchemin K; Department of Biology, Texas A&M University, College Station, TX 77843-3474, USA., Collins D; Department of Biology, Texas A&M University, College Station, TX 77843-3474, USA., Scoggin J; Department of Biology, Texas A&M University, College Station, TX 77843-3474, USA., Scott J; Department of Biology, Texas A&M University, College Station, TX 77843-3474, USA., Gomer RH; Department of Biology, Texas A&M University, College Station, TX 77843-3474, USA. |
| المصدر: | Journal of cell science [J Cell Sci] 2024 Aug 01; Vol. 137 (15). Date of Electronic Publication: 2024 Aug 01. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Company of Biologists Country of Publication: England NLM ID: 0052457 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1477-9137 (Electronic) Linking ISSN: 00219533 NLM ISO Abbreviation: J Cell Sci Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Cambridge : Company of Biologists Original Publication: London. |
| مواضيع طبية MeSH: | Dictyostelium*/metabolism , Dictyostelium*/genetics , Dictyostelium*/enzymology , PTEN Phosphohydrolase*/metabolism , PTEN Phosphohydrolase*/genetics , Protozoan Proteins*/metabolism , Protozoan Proteins*/genetics , Phosphatidylinositol Phosphates*/metabolism, Phosphoric Monoester Hydrolases/metabolism ; Phosphoric Monoester Hydrolases/genetics ; Phosphatidylinositol 4,5-Diphosphate/metabolism ; Chemotaxis ; Signal Transduction ; ras Proteins/metabolism |
| مستخلص: | Little is known about eukaryotic chemorepulsion. The enzymes phosphatase and tensin homolog (PTEN) and CnrN dephosphorylate phosphatidylinositol 3,4,5-trisphosphate [PI(3,4,5)P3] to phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2]. Dictyostelium discoideum cells require both PTEN and CnrN to induce chemorepulsion of cells away from the secreted chemorepellent protein AprA. How D. discoideum cells utilize two proteins with redundant phosphatase activities in response to AprA is unclear. Here, we show that D. discoideum cells require both PTEN and CnrN to locally inhibit Ras activation, decrease basal levels of PI(3,4,5)P3 and increase basal numbers of macropinosomes, and AprA prevents this increase. AprA requires both PTEN and CnrN to increase PI(4,5)P2 levels, decrease PI(3,4,5)P3 levels, inhibit proliferation, decrease myosin II phosphorylation and increase filopod sizes. PTEN, but not CnrN, decreases basal levels of PI(4,5)P2, and AprA requires PTEN, but not CnrN, to induce cell roundness. Together, our results suggest that CnrN and PTEN play unique roles in AprA-induced chemorepulsion. Competing Interests: Competing interests The authors declare no competing or financial interests. (© 2024. Published by The Company of Biologists Ltd.) |
| التعليقات: | Update of: bioRxiv. 2024 Feb 26:2024.02.23.581751. doi: 10.1101/2024.02.23.581751. (PMID: 38464111) |
| معلومات مُعتمدة: | GM118355 United States NH NIH HHS; GM118355 United States NH NIH HHS; Texas A&M University |
| فهرسة مساهمة: | Keywords: Dictyostelium; Chemorepulsion; Chemotaxis; Cytoskeleton; Migration; PTEN; Phosphatidylinositol; Phosphoinositide |
| المشرفين على المادة: | EC 3.1.3.67 (PTEN Phosphohydrolase) 0 (Protozoan Proteins) 0 (Phosphatidylinositol Phosphates) 0 (phosphatidylinositol 3,4,5-triphosphate) EC 3.1.3.2 (Phosphoric Monoester Hydrolases) 0 (Phosphatidylinositol 4,5-Diphosphate) EC 3.6.5.2 (ras Proteins) |
| تواريخ الأحداث: | Date Created: 20240628 Date Completed: 20240801 Latest Revision: 20240804 |
| رمز التحديث: | 20240804 |
| DOI: | 10.1242/jcs.262054 |
| PMID: | 38940195 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1477-9137 |
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| DOI: | 10.1242/jcs.262054 |