دورية أكاديمية
أعرض في EDS

DNA Nanocage-Assisted Size-Selective Recognition and Quantification toward Low-Mass Soluble β-Amyloid Oligomers.

التفاصيل البيبلوغرافية
العنوان: DNA Nanocage-Assisted Size-Selective Recognition and Quantification toward Low-Mass Soluble β-Amyloid Oligomers.
المؤلفون: Chen J; College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, P. R. China., Liu Q; College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, P. R. China., Fu Y; College of Chemistry and Chemical Engineering, Hunan University, Changsha 410083, P. R. China., Xiang J; College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, P. R. China.
المصدر: Analytical chemistry [Anal Chem] 2024 Jul 16; Vol. 96 (28), pp. 11397-11403. Date of Electronic Publication: 2024 Jun 28.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: American Chemical Society Country of Publication: United States NLM ID: 0370536 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1520-6882 (Electronic) Linking ISSN: 00032700 NLM ISO Abbreviation: Anal Chem Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Washington, American Chemical Society.
مواضيع طبية MeSH: Amyloid beta-Peptides*/analysis , Amyloid beta-Peptides*/blood , Amyloid beta-Peptides*/chemistry , DNA*/chemistry, Humans ; Nanostructures/chemistry ; Particle Size ; Electrochemical Techniques/methods ; Alzheimer Disease/diagnosis ; Alzheimer Disease/metabolism ; Alzheimer Disease/blood ; Biosensing Techniques/methods ; Solubility ; Aptamers, Nucleotide/chemistry ; Molecular Weight
مستخلص: Low-mass soluble β-amyloid peptide oligomers (LSAβOs) play a crucial role in the pathogenesis of Alzheimer's disease. However, these oligomers exhibit heterogeneity in terms of structure, stability, and stoichiometry, and their abundance in biofluids is low, making accurate identification challenging. In this study, we developed a DNA nanocage-assisted method for selective sizing and sensitive quantification of LSAβOs in serum. Using LSAβO less than 10 kDa (LSAβO 10kD ) and less than 30 kDa (LSAβO 30kD ) as models, the size-matching rules between DNA nanocages and LSAβOs were investigated, and two appropriate nanocages were selected for the detection of two LSAβOs, respectively. Both nanocages were functionalized by encapsulating oligomer's aptamer and a complementary sequence within their cavities. Once the LSAβO entered the corresponding nanocage cavity, the complementary sequence was released, triggering a hybridization chain reaction on an electrochemical sensing platform. The system achieved size-selective discrimination of LSAβO 10kD with a linear range of 10-150 pM and LSAβO 30kD with a linear range of 15-150 pM. Real sample testing confirmed the applicability of the method for blood-based diagnosis. The DNA nanocage-assisted electrochemical analysis platform provides an accurate, highly selective, and sensitive approach for oligomer analysis, which is significant for amyloid protein research and related disease diagnosis.
المشرفين على المادة: 0 (Amyloid beta-Peptides)
9007-49-2 (DNA)
0 (Aptamers, Nucleotide)
تواريخ الأحداث: Date Created: 20240628 Date Completed: 20240716 Latest Revision: 20240805
رمز التحديث: 20240806
DOI: 10.1021/acs.analchem.4c01465
PMID: 38940533
قاعدة البيانات: MEDLINE
الوصف
تدمد:1520-6882
DOI:10.1021/acs.analchem.4c01465