دورية أكاديمية
أعرض في EDS

The dexd/H-box helicase DHX40 is a novel negative regulator during GCRV infection via targeting the host RLR helicases and viral nonstructural protein NS38.

التفاصيل البيبلوغرافية
العنوان: The dexd/H-box helicase DHX40 is a novel negative regulator during GCRV infection via targeting the host RLR helicases and viral nonstructural protein NS38.
المؤلفون: Liu WJ; College of life sciences, Jiangxi Normal university, Nanchang, Jiangxi, China; Key Laboratory of Aquaculture Disease Control, Ministry of Agriculture, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China., Chen Y; Key Laboratory of Aquaculture Disease Control, Ministry of Agriculture, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China; University of Chinese Academy of Sciences, Beijing 100049, China., Liu Y; College of life sciences, Jiangxi Normal university, Nanchang, Jiangxi, China. Electronic address: yiliusan@jxnu.edu.cn., Chang MX; Key Laboratory of Aquaculture Disease Control, Ministry of Agriculture, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China; University of Chinese Academy of Sciences, Beijing 100049, China. Electronic address: mingxianchang@ihb.ac.cn.
المصدر: Fish & shellfish immunology [Fish Shellfish Immunol] 2024 Aug; Vol. 151, pp. 109730. Date of Electronic Publication: 2024 Jun 26.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Academic Press Country of Publication: England NLM ID: 9505220 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1095-9947 (Electronic) Linking ISSN: 10504648 NLM ISO Abbreviation: Fish Shellfish Immunol Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London ; New York : Academic Press, c1991-
مواضيع طبية MeSH: Viral Nonstructural Proteins*/genetics , Viral Nonstructural Proteins*/immunology , Viral Nonstructural Proteins*/metabolism , Fish Diseases*/immunology , Fish Diseases*/virology , Fish Proteins*/genetics , Fish Proteins*/immunology , Carps*/immunology , Carps*/genetics , Reoviridae Infections*/veterinary , Reoviridae Infections*/immunology , Reoviridae*/physiology , DEAD-box RNA Helicases*/genetics , DEAD-box RNA Helicases*/immunology , DEAD-box RNA Helicases*/metabolism , Immunity, Innate*/genetics, Animals ; RNA Helicases/genetics ; RNA Helicases/metabolism ; RNA Helicases/immunology ; Gene Expression Regulation/immunology
مستخلص: RLR helicases RIG-I and MDA5, which are known as pattern recognition receptors to sense cytoplasmic viral RNAs and trigger antiviral immune responses, are DExD/H-box helicases. In teleost, whether and how non-RLR helicases regulate RLR helicases to affect viral infection remains unclear. Here, we report that the non-RLR helicase DHX40 from grass carp (namely gcDHX40) is a negative regulator of grass carp reovirus (GCRV) infection and RLR-mediated type I IFN production. GcDHX40 was a cytoplasmic protein. Ectopic expression of gcDHX40 facilitated GCRV replication and suppressed type I IFN production induced by GCRV infection and by those genes involved the RLR antiviral signaling pathway. Mechanistically, gcDHX40 promoted the generation of viral inclusion bodies (VIBs) by interacting with the NS38 protein of GCRV. Additionally, gcDHX40 interacted with RLR helicase, and impaired the formation of RLR-MAVS functional complexes. Taken together, our results indicate that gcDHX40 is a novel important proviral host factor involving in promoting the generation of GCRV VIBs and inhibiting the production of RLR-mediated type I IFNs.
(Copyright © 2024 Elsevier Ltd. All rights reserved.)
فهرسة مساهمة: Keywords: DHX40; GCRV infection; Negative regulation; RLRs
المشرفين على المادة: 0 (Viral Nonstructural Proteins)
0 (Fish Proteins)
EC 3.6.4.13 (DEAD-box RNA Helicases)
EC 3.6.4.13 (RNA Helicases)
تواريخ الأحداث: Date Created: 20240628 Date Completed: 20240720 Latest Revision: 20240820
رمز التحديث: 20240820
DOI: 10.1016/j.fsi.2024.109730
PMID: 38942250
قاعدة البيانات: MEDLINE
الوصف
تدمد:1095-9947
DOI:10.1016/j.fsi.2024.109730