دورية أكاديمية
Protocol for fabrication of nanosubstrate embedded with nanogroove topography coated by a layer of nanocomposite for neuronal differentiation.
| العنوان: | Protocol for fabrication of nanosubstrate embedded with nanogroove topography coated by a layer of nanocomposite for neuronal differentiation. |
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| المؤلفون: | Afsharian MH; Department of Biophysics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran 14115-154, Iran., Mahdavian R; Department of Biophysics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran 14115-154, Iran., Allahverdi A; Department of Biophysics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran 14115-154, Iran. Electronic address: a-allahverdi@modares.ac.ir. |
| المصدر: | STAR protocols [STAR Protoc] 2024 Sep 20; Vol. 5 (3), pp. 103166. Date of Electronic Publication: 2024 Jun 28. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Cell Press Country of Publication: United States NLM ID: 101769501 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2666-1667 (Electronic) Linking ISSN: 26661667 NLM ISO Abbreviation: STAR Protoc Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: [Cambridge, MA] : Cell Press, [2020]- |
| مواضيع طبية MeSH: | Nanocomposites*/chemistry , Neurons*/cytology , Cell Differentiation* , Chitosan*/chemistry, Dimethylpolysiloxanes/chemistry ; Aniline Compounds/chemistry ; Animals ; Humans |
| مستخلص: | Neurodegenerative diseases mainly affect the vital characteristics of neurons, including axons. The fabricated nanotopographies can cause axonal regeneration manipulating the migration and differentiation of cells. Here, we present a protocol for the fabrication of nanosubstrate incorporated with nanogroove topography with a coated layer of polyaniline-chitosan (PANI-C) nanocomposite. We describe steps for investigating differences between bulk polydimethylsiloxane (PDMS) sheets and embedding sheets with nanogrooves. We then detail procedures for coating with a PANI-C nanocomposite layer on the substrate. For complete details on the use and execution of this protocol, please refer to Afsharian et al. 1 . Competing Interests: Declaration of interests The authors declare no competing interests. (Copyright © 2024 The Author(s). Published by Elsevier Inc. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Atomic Force Microscopy (AFM); Cell Biology; Cell Differentiation |
| المشرفين على المادة: | 9012-76-4 (Chitosan) 0 (Dimethylpolysiloxanes) 0 (Aniline Compounds) 0 (polyaniline) 63148-62-9 (baysilon) |
| تواريخ الأحداث: | Date Created: 20240629 Date Completed: 20240926 Latest Revision: 20240926 |
| رمز التحديث: | 20240926 |
| مُعرف محوري في PubMed: | PMC11262161 |
| DOI: | 10.1016/j.xpro.2024.103166 |
| PMID: | 38943647 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 2666-1667 |
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| DOI: | 10.1016/j.xpro.2024.103166 |