دورية أكاديمية
Bidirectional Optical Control of Proton Motive Force in Escherichia coli Using Microbial Rhodopsins.
| العنوان: | Bidirectional Optical Control of Proton Motive Force in Escherichia coli Using Microbial Rhodopsins. |
|---|---|
| المؤلفون: | Nakanishi K; Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Okayama 700-8530, Japan., Kojima K; Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Okayama 700-8530, Japan.; Faculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Okayama 700-8530, Japan., Sowa Y; Department of Frontier Bioscience and Research Center for Micro-Nano Technology, Hosei University, Tokyo 184-8584, Japan., Sudo Y; Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Okayama 700-8530, Japan.; Faculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Okayama 700-8530, Japan. |
| المصدر: | The journal of physical chemistry. B [J Phys Chem B] 2024 Jul 11; Vol. 128 (27), pp. 6509-6517. Date of Electronic Publication: 2024 Jul 01. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Chemical Society Country of Publication: United States NLM ID: 101157530 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1520-5207 (Electronic) Linking ISSN: 15205207 NLM ISO Abbreviation: J Phys Chem B Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Washington, D.C. : American Chemical Society, c1997- |
| مواضيع طبية MeSH: | Escherichia coli*/metabolism , Rhodopsins, Microbial*/chemistry , Rhodopsins, Microbial*/metabolism , Proton-Motive Force*, Light ; Proton Pumps/metabolism ; Proton Pumps/chemistry |
| مستخلص: | Proton (H + ) motive force (PMF) serves as the energy source for the flagellar motor rotation, crucial for microbial motility. Here, to control PMF using light, we introduced light-driven inward and outward proton pump rhodopsins, Rm XeR and AR3, into Escherichia coli . The motility of E. coli cells expressing Rm XeR and AR3 significantly decreased and increased upon illumination, respectively. Tethered cell experiments revealed that, upon illumination, the torque of the flagellar motor decreased to nearly zero (28 pN nm) with Rm XeR, while it increased to 1170 pN nm with AR3. These alterations in PMF correspond to +146 mV ( Rm XeR) and -140 mV (AR3), respectively. Thus, bidirectional optical control of PMF in E. coli was successfully achieved by using proton pump rhodopsins. This system holds a potential for enhancing our understanding of the roles of PMF in various biological functions. |
| المشرفين على المادة: | 0 (Rhodopsins, Microbial) 0 (Proton Pumps) |
| تواريخ الأحداث: | Date Created: 20240701 Date Completed: 20240711 Latest Revision: 20240711 |
| رمز التحديث: | 20240711 |
| DOI: | 10.1021/acs.jpcb.4c03027 |
| PMID: | 38949422 |
| قاعدة البيانات: | MEDLINE |
Find this issue from the American Chemical Society | تدمد: | 1520-5207 |
|---|---|
| DOI: | 10.1021/acs.jpcb.4c03027 |