دورية أكاديمية
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Implicating clinical utility of altered expression of PTCH1 & SMO in oral squamous cell carcinoma.

التفاصيل البيبلوغرافية
العنوان: Implicating clinical utility of altered expression of PTCH1 & SMO in oral squamous cell carcinoma.
المؤلفون: Patel HV; Molecular Diagnostic and Research Lab-3, Department of Cancer Biology, The Gujarat Cancer and Research Institute, Ahmedabad, Gujarat, 380016, India.; Gujarat University, Ahmedabad, Gujarat, India., Joshi JS; Molecular Diagnostic and Research Lab-3, Department of Cancer Biology, The Gujarat Cancer and Research Institute, Ahmedabad, Gujarat, 380016, India., Shah FD; Molecular Diagnostic and Research Lab-3, Department of Cancer Biology, The Gujarat Cancer and Research Institute, Ahmedabad, Gujarat, 380016, India. franky.shah@gcriindia.org.
المصدر: Journal of molecular histology [J Mol Histol] 2024 Aug; Vol. 55 (4), pp. 379-389. Date of Electronic Publication: 2024 Jul 01.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Springer Netherlands Country of Publication: Netherlands NLM ID: 101193653 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1567-2387 (Electronic) Linking ISSN: 15672379 NLM ISO Abbreviation: J Mol Histol Subsets: MEDLINE
أسماء مطبوعة: Publication: Dordrecht, The Netherlands : Springer Netherlands
Original Publication: Dordrecht, The Netherlands : Kluwer Academic Publishers, 2004-
مواضيع طبية MeSH: Patched-1 Receptor*/genetics , Patched-1 Receptor*/metabolism , Mouth Neoplasms*/genetics , Mouth Neoplasms*/pathology , Mouth Neoplasms*/metabolism , Mouth Neoplasms*/mortality , Mouth Neoplasms*/diagnosis , Smoothened Receptor*/genetics , Smoothened Receptor*/metabolism , Carcinoma, Squamous Cell*/genetics , Carcinoma, Squamous Cell*/metabolism , Carcinoma, Squamous Cell*/pathology , Carcinoma, Squamous Cell*/mortality , Carcinoma, Squamous Cell*/diagnosis, Humans ; Female ; Male ; Middle Aged ; Adult ; Aged ; Gene Expression Regulation, Neoplastic ; Prognosis ; Biomarkers, Tumor/genetics ; Biomarkers, Tumor/metabolism ; Kaplan-Meier Estimate ; ROC Curve ; Lymphatic Metastasis/genetics ; Neoplasm Staging
مستخلص: Introduction: Oral cancer poses a significant burden on public health in India, with higher incidence and mortality rates. Despite advancements in treatment modalities, prognosis remains poor due to factors such as localized recurrence and lymph node metastasis, potentially influenced by cancer stem cells. Among signaling pathways implicated in CSC regulation, the Hedgehog pathway plays a crucial role in oral squamous cell carcinoma (OSCC).
Material & Methods: 97 OSCC patients' tissue samples were collected and subjected to RNA isolation, cDNA synthesis and quantitative real-time PCR to analyze PTCH1 and SMO expression. Protein expression was assessed through immunohistochemistry. Clinicopathological parameters were correlated with gene and protein expression. Statistical analysis included Pearson chi-square tests, co-relation co-efficient tests, Kaplan-Meier survival analysis and ROC curve analysis.
Results: PTCH1 expression correlated with lymphatic permeation (p = 0.002) and tumor stage (p = 0.002), while SMO expression correlated with lymph node status (p = 0.034) and tumor stage (p = 0.021). PTCH1 gene expression correlated with lymph node status (p = 0.024). High PTCH1 gene expression was associated with shorter survival in tongue cancer patients. ROC curve analysis indicated diagnostic potential for PTCH1 and SMO gene and cytoplasmic SMO expression in distinguishing malignant tissues from adjacent normal tissues.
Conclusion: PTCH1 and SMO play a crucial role in oral cancer progression, correlating with tumor stages and metastatic potential. Despite not directly influencing overall survival, PTCH1 expression at specific anatomical sites hints at its prognostic implications. PTCH1 and SMO exhibit diagnostic potential, suggesting their utility as molecular markers in oral cancer management and therapeutic strategies.
(© 2024. The Author(s), under exclusive licence to Springer Nature B.V.)
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فهرسة مساهمة: Keywords: Gene expression; Oral cancer; PTCH1; Protein expression; SMO
المشرفين على المادة: 0 (Patched-1 Receptor)
0 (Smoothened Receptor)
0 (PTCH1 protein, human)
0 (SMO protein, human)
0 (Biomarkers, Tumor)
تواريخ الأحداث: Date Created: 20240702 Date Completed: 20240807 Latest Revision: 20240917
رمز التحديث: 20240917
DOI: 10.1007/s10735-024-10215-3
PMID: 38954185
قاعدة البيانات: MEDLINE
الوصف
تدمد:1567-2387
DOI:10.1007/s10735-024-10215-3