دورية أكاديمية
Intestinal organoids to model Salmonella infection and its impact on progenitors.
| العنوان: | Intestinal organoids to model Salmonella infection and its impact on progenitors. |
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| المؤلفون: | Yan J; IRSD - Institut de Recherche en Santé Digestive, Université de Toulouse, INSERM, INRAE, ENVT, UPS, Toulouse, France.; Department of Gastroenterology, The Second Xiangya Hospital of Central South University, Changsha, China.; Research Center of Digestive Disease, Central South University, Changsha, China., Racaud-Sultan C; IRSD - Institut de Recherche en Santé Digestive, Université de Toulouse, INSERM, INRAE, ENVT, UPS, Toulouse, France., Pezier T; ISP, INRAE, Université de Tours, 37380, Nouzilly, France., Edir A; IRSD - Institut de Recherche en Santé Digestive, Université de Toulouse, INSERM, INRAE, ENVT, UPS, Toulouse, France., Rolland C; IRSD - Institut de Recherche en Santé Digestive, Université de Toulouse, INSERM, INRAE, ENVT, UPS, Toulouse, France., Claverie C; IRSD - Institut de Recherche en Santé Digestive, Université de Toulouse, INSERM, INRAE, ENVT, UPS, Toulouse, France., Burlaud-Gaillard J; Plateforme IBISA de Microscopie Electronique, Université de Tours, CHRU de Tours, Tours, France., Olivier M; ISP, INRAE, Université de Tours, 37380, Nouzilly, France., Velge P; ISP, INRAE, Université de Tours, 37380, Nouzilly, France., Lacroix-Lamandé S; ISP, INRAE, Université de Tours, 37380, Nouzilly, France., Vergnolle N; IRSD - Institut de Recherche en Santé Digestive, Université de Toulouse, INSERM, INRAE, ENVT, UPS, Toulouse, France., Wiedemann A; IRSD - Institut de Recherche en Santé Digestive, Université de Toulouse, INSERM, INRAE, ENVT, UPS, Toulouse, France. agnes.wiedemann@inrae.fr.; ISP, INRAE, Université de Tours, 37380, Nouzilly, France. agnes.wiedemann@inrae.fr. |
| المصدر: | Scientific reports [Sci Rep] 2024 Jul 02; Vol. 14 (1), pp. 15160. Date of Electronic Publication: 2024 Jul 02. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: London : Nature Publishing Group, copyright 2011- |
| مواضيع طبية MeSH: | Organoids*/microbiology , Stem Cells*/metabolism , Salmonella typhimurium*/pathogenicity , Salmonella typhimurium*/physiology , Cell Differentiation* , Salmonella Infections*/microbiology , Salmonella Infections*/pathology , ErbB Receptors*/metabolism , ErbB Receptors*/genetics, Animals ; Mice ; Intestinal Mucosa/microbiology ; Intestinal Mucosa/pathology ; Cell Proliferation ; Disease Models, Animal ; Mice, Inbred C57BL |
| مستخلص: | In order to survive and replicate, Salmonella has evolved mechanisms to gain access to intestinal epithelial cells of the crypt. However, the impact of Salmonella Typhimurium on stem cells and progenitors, which are responsible for the ability of the intestinal epithelium to renew and protect itself, remains unclear. Given that intestinal organoids growth is sustained by stem cells and progenitors activity, we have used this model to document the effects of Salmonella Typhimurium infection on epithelial proliferation and differentiation, and compared it to an in vivo model of Salmonella infection in mice. Among gut segments, the caecum was preferentially targeted by Salmonella. Analysis of infected crypts and organoids demonstrated increased length and size, respectively. mRNA transcription profiles of infected crypts and organoids pointed to upregulated EGFR-dependent signals, associated with a decrease in secretory cell lineage differentiation. To conclude, we show that organoids are suited to mimic the impact of Salmonella on stem cells and progenitors cells, carrying a great potential to drastically reduce the use of animals for scientific studies on that topic. In both models, the EGFR pathway, crucial to stem cells and progenitors proliferation and differentiation, is dysregulated by Salmonella, suggesting that repeated infections might have consequences on crypt integrity and further oncogenesis. (© 2024. The Author(s).) |
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| فهرسة مساهمة: | Keywords: Salmonella; Caecum; EGFR; Organoid; Progenitor; Stem cell |
| المشرفين على المادة: | EC 2.7.10.1 (ErbB Receptors) |
| تواريخ الأحداث: | Date Created: 20240702 Date Completed: 20240702 Latest Revision: 20240705 |
| رمز التحديث: | 20240705 |
| مُعرف محوري في PubMed: | PMC11219929 |
| DOI: | 10.1038/s41598-024-65485-4 |
| PMID: | 38956132 |
| قاعدة البيانات: | MEDLINE |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 2045-2322 |
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| DOI: | 10.1038/s41598-024-65485-4 |