دورية أكاديمية
أعرض في EDS

Lycopene alleviates Deoxynivalenol-induced toxicity in Porcine intestinal epithelial cells by mediating mitochondrial function.

التفاصيل البيبلوغرافية
العنوان: Lycopene alleviates Deoxynivalenol-induced toxicity in Porcine intestinal epithelial cells by mediating mitochondrial function.
المؤلفون: Wang J; Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design, college of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China., Lu F; Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design, college of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China., Gu S; Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design, college of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China., Cao C; Wens Foodstuff Group Co., Ltd., Xinxing 527400, China., Xiao Y; Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design, college of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China., Bao W; Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design, college of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China; International Joint Research Laboratory in Universities of Jiangsu Province of China for Domestic Animal Germplasm Resources and Genetic Improvement, Yangzhou University, Yangzhou, Jiangsu 225009, China., Wang H; Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design, college of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China; International Joint Research Laboratory in Universities of Jiangsu Province of China for Domestic Animal Germplasm Resources and Genetic Improvement, Yangzhou University, Yangzhou, Jiangsu 225009, China. Electronic address: China.hyfiwang@yzu.edu.cn.
المصدر: Toxicology [Toxicology] 2024 Aug; Vol. 506, pp. 153880. Date of Electronic Publication: 2024 Jul 02.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Elsevier Country of Publication: Ireland NLM ID: 0361055 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-3185 (Electronic) Linking ISSN: 0300483X NLM ISO Abbreviation: Toxicology Subsets: MEDLINE
أسماء مطبوعة: Publication: Limerick : Elsevier
Original Publication: Amsterdam. Elsevier/North-Holland.
مواضيع طبية MeSH: Trichothecenes*/toxicity , Mitochondria*/drug effects , Mitochondria*/metabolism , Lycopene*/pharmacology , Reactive Oxygen Species*/metabolism , Membrane Potential, Mitochondrial*/drug effects , Cell Survival*/drug effects , Epithelial Cells*/drug effects , Epithelial Cells*/metabolism , Oxidative Stress*/drug effects, Animals ; Swine ; Cell Line ; Apoptosis/drug effects ; Antioxidants/pharmacology ; Intestinal Mucosa/drug effects ; Intestinal Mucosa/metabolism
مستخلص: Deoxynivalenol (DON) is widely found in food and feed, posing a threat to human and animal health. Lycopene (Lyc) is a natural plant extracts with significant antioxidant properties. This study was conducted to investigate the protective effects of Lyc on IPEC-J2 cells upon DON exposure. The detection of cell viability and trypan blue staining showed that Lyc alleviated cell damage and decreased cell apoptotic rate induced by DON. The analysis of reactive oxygen species (ROS) level and antioxidant parameter measurements showed that Lyc significantly down-regulated the content of ROS and restored antioxidant enzyme activity. Furthermore, mitochondrial membrane potential (ΔΨm) detection, mitochondrial DNA copy number (mtDNAcn) assay and adenosine triphosphate (ATP) concentration detection showed Lyc improved mitochondrial function after DON exposure. The results of transcriptome analysis, ROS detection and CCK8 assay suggested that Lyc may activated the oxidative phosphorylation (OXPHOS) to improve mitochondrial function. Conclusively, our results suggested that Lyc alleviated DON-induced oxidative stress by improving mitochondrial function through OXPHOS signaling pathway.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
فهرسة مساهمة: Keywords: Deoxynivalenol; Lycopene; Mitochondrion; Oxidative phosphorylation; Oxidative stress
المشرفين على المادة: 0 (Trichothecenes)
JT37HYP23V (deoxynivalenol)
SB0N2N0WV6 (Lycopene)
0 (Reactive Oxygen Species)
0 (Antioxidants)
تواريخ الأحداث: Date Created: 20240703 Date Completed: 20240722 Latest Revision: 20240722
رمز التحديث: 20240723
DOI: 10.1016/j.tox.2024.153880
PMID: 38960307
قاعدة البيانات: MEDLINE
الوصف
تدمد:1879-3185
DOI:10.1016/j.tox.2024.153880