دورية أكاديمية
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Optimal conditions of algal breeding using neutral beam and applying it to breed Euglena gracilis strains with improved lipid accumulation.

التفاصيل البيبلوغرافية
العنوان: Optimal conditions of algal breeding using neutral beam and applying it to breed Euglena gracilis strains with improved lipid accumulation.
المؤلفون: Imamura S; Space Environment and Energy Laboratories, Nippon Telegraph and Telephone Corporation, Musashino-shi, 180-8585, Japan. sousuke.imamura@ntt.com., Yamada K; Advanced Science Research Institute, Euglena Co., Ltd., Yokohama-shi, 230-0045, Japan., Takebe H; Space Environment and Energy Laboratories, Nippon Telegraph and Telephone Corporation, Musashino-shi, 180-8585, Japan., Kiuchi R; Space Environment and Energy Laboratories, Nippon Telegraph and Telephone Corporation, Musashino-shi, 180-8585, Japan., Iwashita H; Space Environment and Energy Laboratories, Nippon Telegraph and Telephone Corporation, Musashino-shi, 180-8585, Japan., Toyokawa C; Advanced Science Research Institute, Euglena Co., Ltd., Yokohama-shi, 230-0045, Japan., Suzuki K; Advanced Science Research Institute, Euglena Co., Ltd., Yokohama-shi, 230-0045, Japan., Sakurai A; Space Environment and Energy Laboratories, Nippon Telegraph and Telephone Corporation, Musashino-shi, 180-8585, Japan., Takaya K; Space Environment and Energy Laboratories, Nippon Telegraph and Telephone Corporation, Musashino-shi, 180-8585, Japan.
المصدر: Scientific reports [Sci Rep] 2024 Jul 03; Vol. 14 (1), pp. 14716. Date of Electronic Publication: 2024 Jul 03.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London : Nature Publishing Group, copyright 2011-
مواضيع طبية MeSH: Euglena gracilis*/genetics , Euglena gracilis*/radiation effects , Euglena gracilis*/metabolism , Lipid Metabolism*/radiation effects , Lipid Metabolism*/genetics, Microalgae/genetics ; Microalgae/radiation effects ; Microalgae/metabolism ; Neutrons ; Mutation ; Biomass ; Lipids ; Biofuels
مستخلص: Microalgae are considered to be more useful and effective to use in biomass production than other photosynthesis organisms. However, microalgae need to be altered to acquire more desirable traits for the relevant purpose. Although neutron radiation is known to induce DNA mutations, there have been few studies on its application to microalgae, and the optimal relationship between irradiation intensity and mutation occurrence has not been established. In this study, using the unicellular red alga Cyanidioschyzon merolae as a model, we analyzed the relationship between the absorbed dose of two types of neutrons, high-energy (above 1 MeV) and thermal (around 25 meV) neutrons, and mutation occurrence while monitoring mutations in URA5.3 gene encoding UMP synthase. As a result, the highest mutational occurrence was observed when the cells were irradiated with 20 Gy of high-energy neutrons and 13 Gy of thermal neutrons. Using these optimal neutron irradiation conditions, we next attempted to improve the lipid accumulation of Euglena gracilis, which is a candidate strain for biofuel feedstock production. As a result, we obtained several strains with a maximum 1.3-fold increase in lipid accumulation compared with the wild-type. These results indicate that microalgae breeding by neutron irradiation is effective.
(© 2024. The Author(s).)
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فهرسة مساهمة: Keywords: Algal oil; Microalga; Mutagenesis; Neutron beam; Strain development
المشرفين على المادة: 0 (Lipids)
0 (Biofuels)
تواريخ الأحداث: Date Created: 20240703 Date Completed: 20240703 Latest Revision: 20240706
رمز التحديث: 20240706
مُعرف محوري في PubMed: PMC11222385
DOI: 10.1038/s41598-024-65175-1
PMID: 38961078
قاعدة البيانات: MEDLINE
الوصف
تدمد:2045-2322
DOI:10.1038/s41598-024-65175-1