دورية أكاديمية
Immunogenicity of PvCyRPA, PvCelTOS and Pvs25 chimeric recombinant protein of Plasmodium vivax in murine model.
العنوان: | Immunogenicity of PvCyRPA, PvCelTOS and Pvs25 chimeric recombinant protein of Plasmodium vivax in murine model. |
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المؤلفون: | Matos ADS; Immunoparasitology Laboratory, Oswaldo Cruz Institute (IOC), Oswaldo Cruz Foundation (Fiocruz), Rio de Janeiro, Brazil., Soares IF; Immunoparasitology Laboratory, Oswaldo Cruz Institute (IOC), Oswaldo Cruz Foundation (Fiocruz), Rio de Janeiro, Brazil., Rodrigues-da-Silva RN; Hantaviruses and Rickettsioses Laboratory, IOC, Fiocruz, Rio de Janeiro, Brazil., Rodolphi CM; Parasitology Research Center, Federal University of Juiz de Fora (UFJF), Juiz de Fora, Brazil., Albrecht L; Apicomplexa Research Laboratory, Carlos Chagas Institute, Curitiba, Brazil., Donassolo RA; Apicomplexa Research Laboratory, Carlos Chagas Institute, Curitiba, Brazil., Lopez-Camacho C; Nuffield Department of Medicine, The Jenner Institute, University of Oxford, Oxford, United Kingdom., Ano Bom APD; Immunological Technology Laboratory, Immunobiological Technology Institute (Bio-Manguinhos/Fiocruz), Rio de Janeiro, Brazil., Neves PCDC; Immunological Technology Laboratory, Immunobiological Technology Institute (Bio-Manguinhos/Fiocruz), Rio de Janeiro, Brazil., Conte FP; Eukaryotic Pilot Laboratory, Immunobiological Technology Institute (Bio-Manguinhos/Fiocruz), Rio de Janeiro, Brazil., Pratt-Riccio LR; Malaria Research Laboratory, IOC, Fiocruz, Rio de Janeiro, Brazil., Daniel-Ribeiro CT; Malaria Research Laboratory, IOC, Fiocruz, Rio de Janeiro, Brazil., Totino PRR; Malaria Research Laboratory, IOC, Fiocruz, Rio de Janeiro, Brazil., Lima-Junior JDC; Immunoparasitology Laboratory, Oswaldo Cruz Institute (IOC), Oswaldo Cruz Foundation (Fiocruz), Rio de Janeiro, Brazil. |
المصدر: | Frontiers in immunology [Front Immunol] 2024 Jun 19; Vol. 15, pp. 1392043. Date of Electronic Publication: 2024 Jun 19 (Print Publication: 2024). |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: Frontiers Research Foundation] Country of Publication: Switzerland NLM ID: 101560960 Publication Model: eCollection Cited Medium: Internet ISSN: 1664-3224 (Electronic) Linking ISSN: 16643224 NLM ISO Abbreviation: Front Immunol Subsets: MEDLINE |
أسماء مطبوعة: | Original Publication: [Lausanne : Frontiers Research Foundation] |
مواضيع طبية MeSH: | Plasmodium vivax*/immunology , Plasmodium vivax*/genetics , Antigens, Protozoan*/immunology , Antigens, Protozoan*/genetics , Malaria, Vivax*/immunology , Malaria, Vivax*/prevention & control , Antibodies, Protozoan*/immunology , Mice, Inbred BALB C* , Malaria Vaccines*/immunology , Protozoan Proteins*/immunology , Protozoan Proteins*/genetics, Animals ; Mice ; Female ; Epitopes, B-Lymphocyte/immunology ; Epitopes, B-Lymphocyte/genetics ; Recombinant Fusion Proteins/immunology ; Recombinant Fusion Proteins/genetics ; Disease Models, Animal ; Adjuvants, Immunologic ; Immunogenicity, Vaccine ; Antigens, Surface |
مستخلص: | In the Americas, P. vivax is the predominant causative species of malaria, a debilitating and economically significant disease. Due to the complexity of the malaria parasite life cycle, a vaccine formulation with multiple antigens expressed in various parasite stages may represent an effective approach. Based on this, we previously designed and constructed a chimeric recombinant protein, PvRMC-1, composed by PvCyRPA, PvCelTOS, and Pvs25 epitopes. This chimeric protein was strongly recognized by naturally acquired antibodies from exposed population in the Brazilian Amazon. However, there was no investigation about the induced immune response of PvRMC-1. Therefore, in this work, we evaluated the immunogenicity of this chimeric antigen formulated in three distinct adjuvants: Stimune, AddaVax or Aluminum hydroxide (Al(OH)3) in BALB/c mice. Our results suggested that the chimeric protein PvRMC-1 were capable to generate humoral and cellular responses across all three formulations. Antibodies recognized full-length PvRMC-1 and linear B-cell epitopes from PvCyRPA, PvCelTOS, and Pvs25 individually. Moreover, mice's splenocytes were activated, producing IFN-γ in response to PvCelTOS and PvCyRPA peptide epitopes, affirming T-cell epitopes in the antigen. While aluminum hydroxide showed notable cellular response, Stimune and Addavax induced a more comprehensive immune response, encompassing both cellular and humoral components. Thus, our findings indicate that PvRMC-1 would be a promising multistage vaccine candidate that could advance to further preclinical studies. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2024 Matos, Soares, Rodrigues-da-Silva, Rodolphi, Albrecht, Donassolo, Lopez-Camacho, Ano Bom, Neves, Conte, Pratt-Riccio, Daniel-Ribeiro, Totino and Lima-Junior.) |
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فهرسة مساهمة: | Keywords: BALB/c; immunization; immunogenicity; in silico simulation; multistage chimeric protein; plasmodium vivax; vaccine |
المشرفين على المادة: | 0 (Antigens, Protozoan) 0 (Antibodies, Protozoan) 0 (Malaria Vaccines) 0 (Protozoan Proteins) 0 (Epitopes, B-Lymphocyte) 0 (Recombinant Fusion Proteins) 0 (Pvs25 protein, P vivax) 0 (Adjuvants, Immunologic) 0 (Antigens, Surface) |
تواريخ الأحداث: | Date Created: 20240704 Date Completed: 20240704 Latest Revision: 20240726 |
رمز التحديث: | 20240726 |
مُعرف محوري في PubMed: | PMC11219565 |
DOI: | 10.3389/fimmu.2024.1392043 |
PMID: | 38962015 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1664-3224 |
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DOI: | 10.3389/fimmu.2024.1392043 |