دورية أكاديمية
Essential function of transmembrane transcription factor MYRF in promoting transcription of miRNA lin-4 during C. elegans development.
العنوان: | Essential function of transmembrane transcription factor MYRF in promoting transcription of miRNA lin-4 during C. elegans development. |
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المؤلفون: | Xu Z; School of Life Science and Technology, ShanghaiTech University, Shanghai, China., Wang Z; School of Life Science and Technology, ShanghaiTech University, Shanghai, China., Wang L; School of Life Science and Technology, ShanghaiTech University, Shanghai, China., Qi YB; School of Life Science and Technology, ShanghaiTech University, Shanghai, China. |
المصدر: | ELife [Elife] 2024 Jul 04; Vol. 12. Date of Electronic Publication: 2024 Jul 04. |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: eLife Sciences Publications, Ltd Country of Publication: England NLM ID: 101579614 Publication Model: Electronic Cited Medium: Internet ISSN: 2050-084X (Electronic) Linking ISSN: 2050084X NLM ISO Abbreviation: Elife Subsets: MEDLINE |
أسماء مطبوعة: | Original Publication: Cambridge, UK : eLife Sciences Publications, Ltd., 2012- |
مواضيع طبية MeSH: | Caenorhabditis elegans*/genetics , Caenorhabditis elegans*/metabolism , Caenorhabditis elegans*/growth & development , Caenorhabditis elegans Proteins*/metabolism , Caenorhabditis elegans Proteins*/genetics , Gene Expression Regulation, Developmental* , MicroRNAs*/metabolism , MicroRNAs*/genetics , Transcription Factors*/metabolism , Transcription Factors*/genetics, Animals ; Promoter Regions, Genetic ; Transcription, Genetic ; Membrane Proteins/metabolism ; Membrane Proteins/genetics ; Cell Nucleus/metabolism |
مستخلص: | Precise developmental timing control is essential for organism formation and function, but its mechanisms are unclear. In C. elegans , the microRNA lin-4 critically regulates developmental timing by post-transcriptionally downregulating the larval-stage-fate controller LIN-14. However, the mechanisms triggering the activation of lin-4 expression toward the end of the first larval stage remain unknown. We demonstrate that the transmembrane transcription factor MYRF-1 is necessary for lin-4 activation. MYRF-1 is initially localized on the cell membrane, and its increased cleavage and nuclear accumulation coincide with lin-4 expression timing. MYRF-1 regulates lin-4 expression cell-autonomously and hyperactive MYRF-1 can prematurely drive lin-4 expression in embryos and young first-stage larvae. The tandem lin-4 promoter DNA recruits MYRF-1 GFP to form visible loci in the nucleus, suggesting that MYRF-1 directly binds to the lin-4 promoter. Our findings identify a crucial link in understanding developmental timing regulation and establish MYRF-1 as a key regulator of lin-4 expression. Competing Interests: ZX, ZW, LW, YQ No competing interests declared (© 2023, Xu et al.) |
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معلومات مُعتمدة: | P40 OD010440 United States OD NIH HHS; 31900397 National Science Foundation of China |
فهرسة مساهمة: | Keywords: C. elegans; DD synaptic rewiring; MYRF-2; PAN-1; dauer; developmental biology; intramolecular chaperone; self-cleavage |
سلسلة جزيئية: | Dryad 10.5061/dryad.tqjq2bw71 GEO GSE262766 |
المشرفين على المادة: | 0 (Caenorhabditis elegans Proteins) 0 (MicroRNAs) 0 (Transcription Factors) 0 (lin-4 microRNA, C elegans) 0 (Membrane Proteins) |
تواريخ الأحداث: | Date Created: 20240704 Date Completed: 20240704 Latest Revision: 20240729 |
رمز التحديث: | 20240729 |
مُعرف محوري في PubMed: | PMC11223767 |
DOI: | 10.7554/eLife.89903 |
PMID: | 38963411 |
قاعدة البيانات: | MEDLINE |
تدمد: | 2050-084X |
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DOI: | 10.7554/eLife.89903 |