دورية أكاديمية
Basal release of 6-cyanodopamine from rat isolated vas deferens and its role on the tissue contractility.
| العنوان: | Basal release of 6-cyanodopamine from rat isolated vas deferens and its role on the tissue contractility. |
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| المؤلفون: | Pozzo CFSD; Department of Pharmacology, Faculty of Medical Sciences, State University of Campinas (UNICAMP), Campinas, Brazil., Junior JEM; Department of Surgery, Faculty of Medicine of Jundiaí, Jundiaí, Brazil., Britto-Júnior J; Department of Pharmacology, Faculty of Medical Sciences, State University of Campinas (UNICAMP), Campinas, Brazil. josebrittojr@dac.unicamp.br., Badin JFA; Department of Pharmacology, Faculty of Medical Sciences, State University of Campinas (UNICAMP), Campinas, Brazil., de Souza VB; Department of Pharmacology, Faculty of Medical Sciences, State University of Campinas (UNICAMP), Campinas, Brazil., Schenka AA; Department of Pharmacology, Faculty of Medical Sciences, State University of Campinas (UNICAMP), Campinas, Brazil., Peterson LW; Department of Chemistry, Rhodes College, Memphis, Tennessee, USA., Fregonesi A; Department of Surgery, Faculty of Medicine of Jundiaí, Jundiaí, Brazil.; Division of Urology, Department of Surgery, Faculty of Medical Sciences, State University of Campinas (UNICAMP), Campinas, Brazil., Antunes E; Department of Pharmacology, Faculty of Medical Sciences, State University of Campinas (UNICAMP), Campinas, Brazil., De Nucci G; Department of Pharmacology, Faculty of Medical Sciences, State University of Campinas (UNICAMP), Campinas, Brazil.; Department of Pharmacology, Institute of Biomedical Sciences, University of São Paulo (USP), São Paulo, Brazil. |
| المصدر: | Pflugers Archiv : European journal of physiology [Pflugers Arch] 2024 Aug; Vol. 476 (8), pp. 1263-1277. Date of Electronic Publication: 2024 Jul 04. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Springer Country of Publication: Germany NLM ID: 0154720 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1432-2013 (Electronic) Linking ISSN: 00316768 NLM ISO Abbreviation: Pflugers Arch Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Berlin, New York, Springer. |
| مواضيع طبية MeSH: | Vas Deferens*/drug effects , Vas Deferens*/metabolism , Vas Deferens*/physiology , Muscle Contraction*/drug effects , Dopamine*/metabolism , Dopamine*/pharmacology, Male ; Animals ; Rats ; Rats, Wistar ; Norepinephrine/pharmacology ; Norepinephrine/metabolism ; Muscle, Smooth/drug effects ; Muscle, Smooth/metabolism ; Muscle, Smooth/physiology ; Electric Stimulation ; Epinephrine/pharmacology ; Tyrosine 3-Monooxygenase/metabolism |
| مستخلص: | 6-Cyanodopamine is a novel catecholamine released from rabbit isolated heart. However, it is not known whether this catecholamine presents any biological activity. Here, it was evaluated whether 6-cyanodopamine (6-CYD) is released from rat vas deferens and its effect on this tissue contractility. Basal release of 6-CYD, 6-nitrodopamine (6-ND), 6-bromodopamine, 6-nitrodopa, and 6-nitroadrenaline from vas deferens were quantified by LC-MS/MS. Electric-field stimulation (EFS) and concentration-response curves to noradrenaline, adrenaline, and dopamine of the rat isolated epididymal vas deferens (RIEVD) were performed in the absence and presence of 6-CYD and /or 6-ND. Expression of tyrosine hydroxylase was assessed by immunohistochemistry. The rat isolated vas deferens released significant amounts of both 6-CYD and 6-ND. The voltage-gated sodium channel blocker tetrodotoxin had no effect on the release of 6-CYD, but it virtually abolished 6-ND release. 6-CYD alone exhibited a negligible RIEVD contractile activity; however, at 10 nM, 6-CYD significantly potentiated the noradrenaline- and EFS-induced RIEVD contractions, whereas at 10 and 100 nM, it also significantly potentiated the adrenaline- and dopamine-induced contractions. The potentiation of noradrenaline- and adrenaline-induced contractions by 6-CYD was unaffected by tetrodotoxin. Co-incubation of 6-CYD (100 pM) with 6-ND (10 pM) caused a significant leftward shift and increased the maximal contractile responses to noradrenaline, even in the presence of tetrodotoxin. Immunohistochemistry revealed the presence of tyrosine hydroxylase in both epithelial cell cytoplasm of the mucosae and nerve fibers of RIEVD. The identification of epithelium-derived 6-CYD and its remarkable synergism with catecholamines indicate that epithelial cells may regulate vas deferens smooth muscle contractility. (© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.) |
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| فهرسة مساهمة: | Keywords: S100; ejaculation; high performance liquid chromatography; smooth muscle tonus; synergism; tandem mass spectrometry |
| المشرفين على المادة: | VTD58H1Z2X (Dopamine) X4W3ENH1CV (Norepinephrine) YKH834O4BH (Epinephrine) EC 1.14.16.2 (Tyrosine 3-Monooxygenase) |
| تواريخ الأحداث: | Date Created: 20240704 Date Completed: 20240721 Latest Revision: 20240721 |
| رمز التحديث: | 20240722 |
| DOI: | 10.1007/s00424-024-02985-2 |
| PMID: | 38963545 |
| قاعدة البيانات: | MEDLINE |
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Full Text Finder | تدمد: | 1432-2013 |
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| DOI: | 10.1007/s00424-024-02985-2 |