دورية أكاديمية
Potent synergy and sustained bactericidal activity of polymyxins combined with Gram-positive only class of antibiotics versus four Gram-negative bacteria.
| العنوان: | Potent synergy and sustained bactericidal activity of polymyxins combined with Gram-positive only class of antibiotics versus four Gram-negative bacteria. |
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| المؤلفون: | Wang Y; School of Pharmaceutical Sciences, Southern Medical University, Guangzhou, 510515, China.; Department of Pharmacy, General Hospital of Southern Theatre Command, Guangzhou, 510010, China., Feng J; School of Pharmaceutical Sciences, Southern Medical University, Guangzhou, 510515, China.; Department of Pharmacy, General Hospital of Southern Theatre Command, Guangzhou, 510010, China., Yu J; Graduate School, Guangzhou University of Chinese Medicine, Guangzhou, 510006, China., Wen L; School of Pharmaceutical Sciences, Dali University, Dali, 671003, China., Chen L; Department of Laboratory Medicine, General Hospital of Southern Theatre Command, Guangzhou, 510010, China., An H; Department of Pharmacy, General Hospital of Southern Theatre Command, Guangzhou, 510010, China., Xiao W; Department of Clinical Pharmacy, General Hospital of Southern Theatre Command, Guangzhou, 510010, China., Zhang B; Department of Healthcare, General Hospital of Southern Theatre Command, Guangzhou, 510010, China., Feng H; Department of Healthcare, General Hospital of Southern Theatre Command, Guangzhou, 510010, China., Zhou M; Department of Blood Transfusion Medicine, General Hospital of Southern Theatre Command, Guangzhou, 510010, China., Jiang Z; School of Pharmaceutical Sciences, Southern Medical University, Guangzhou, 510515, China. jandsphy@163.com.; Department of Pharmacy, General Hospital of Southern Theatre Command, Guangzhou, 510010, China. jandsphy@163.com.; Graduate School, Guangzhou University of Chinese Medicine, Guangzhou, 510006, China. jandsphy@163.com. |
| المصدر: | Annals of clinical microbiology and antimicrobials [Ann Clin Microbiol Antimicrob] 2024 Jul 04; Vol. 23 (1), pp. 60. Date of Electronic Publication: 2024 Jul 04. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: BioMed Central Country of Publication: England NLM ID: 101152152 Publication Model: Electronic Cited Medium: Internet ISSN: 1476-0711 (Electronic) Linking ISSN: 14760711 NLM ISO Abbreviation: Ann Clin Microbiol Antimicrob Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: London : BioMed Central, [2002]- |
| مواضيع طبية MeSH: | Drug Synergism* , Anti-Bacterial Agents*/pharmacology , Microbial Sensitivity Tests* , Gram-Negative Bacteria*/drug effects , Polymyxins*/pharmacology , Polymyxin B*/pharmacology , Colistin*/pharmacology, Humans ; Gram-Negative Bacterial Infections/drug therapy ; Gram-Negative Bacterial Infections/microbiology ; Pseudomonas aeruginosa/drug effects |
| مستخلص: | Background: Gram-negative bacteria (GNB) are becoming increasingly resistant to a wide variety of antibiotics. There are currently limited treatments for GNB, and the combination of antibiotics with complementary mechanisms has been reported to be a feasible strategy for treating GNB infection. The inability to cross the GNB outer membrane (OM) is an important reason that a broad spectrum of Gram-positive only class of antibiotics (GPOAs) is lacking. Polymyxins may help GPOAs to permeate by disrupting OM of GNB. Objective: To identify what kind of GPOAs can be aided to broaden their anti-GNB spectrum by polymyxins, we systematically investigated the synergy of eight GPOAs in combination with colistin (COL) and polymyxin B (PMB) against GNB in vitro. Methods: The synergistic effect of COL or PMB and GPOAs combinations against GNB reference strains and clinical isolates were determined by checkerboard tests. The killing kinetics of the combinations were assessed using time-kill assays. Results: In the checkerboard tests, polymyxins-GPOAs combinations exert synergistic effects characterized by species and strain specificity. The synergistic interactions on P. aeruginosa strains are significantly lower than those on strains of A. baumannii, K. pneumoniae and E. coli. Among all the combinations, COL has shown the best synergistic effect in combination with dalbavancin (DAL) or oritavancin (ORI) versus almost all of the strains tested, with FICIs from 0.16 to 0.50 and 0.13 to < 0.28, respectively. In addition, the time-kill assays demonstrated that COL/DAL and COL/ORI had sustained bactericidal activity. Conclusions: Our results indicated that polymyxins could help GPOAs to permeate the OM of specific GNB, thus showed synergistic effects and bactericidal effects in the in vitro assays. In vivo combination studies should be further conducted to validate the results of this study. (© 2024. The Author(s).) |
| References: | ACS Infect Dis. 2022 Sep 9;8(9):1731-1757. (PMID: 35946799) J Antimicrob Chemother. 2011 May;66(5):1047-51. (PMID: 21393131) Antimicrob Agents Chemother. 2012 Apr;56(4):2084-90. (PMID: 22290971) Rev Soc Bras Med Trop. 2014 Jul;47(4):451-6. (PMID: 25229285) Antimicrob Agents Chemother. 2016 Jun 20;60(7):4342-5. (PMID: 27067327) J Biosci. 2021;46:. (PMID: 34475315) Expert Rev Anti Infect Ther. 2023 Apr;21(4):387-429. (PMID: 36820511) Microbiol Spectr. 2023 Feb 21;:e0427522. (PMID: 36802038) J Glob Antimicrob Resist. 2018 Mar;12:124-136. (PMID: 28962863) Nature. 2017 May 18;545(7654):299-304. (PMID: 28489819) J Bacteriol. 2008 Sep;190(17):5981-8. (PMID: 18621901) Antimicrob Agents Chemother. 2008 May;52(5):1597-603. (PMID: 18299406) Antimicrob Agents Chemother. 2010 Dec;54(12):5316-22. (PMID: 20876375) Front Pharmacol. 2022 Apr 26;13:887941. (PMID: 35559242) Antimicrob Agents Chemother. 2012 Sep;56(9):4856-61. (PMID: 22751540) Microbiol Spectr. 2021 Oct 31;9(2):e0018121. (PMID: 34668731) Int J Antimicrob Agents. 2014 Apr;43(4):370-4. (PMID: 24560919) ACS Infect Dis. 2021 Dec 10;7(12):3314-3335. (PMID: 34766746) Microbiol Res. 2024 Jun;283:127679. (PMID: 38508087) Antimicrob Agents Chemother. 2023 Feb 16;67(2):e0137722. (PMID: 36715507) Antimicrob Agents Chemother. 2018 Sep 24;62(10):. (PMID: 30061285) |
| معلومات مُعتمدة: | 2023A1515030153 Natural Science Foundation of Guangdong Province Youth Enhancement Project; 2022NZA001 Key Basic Project of General Hospital of Southern Theatre Command; 2024A03J0644 Science and Technology Program of Guangzhou |
| فهرسة مساهمة: | Keywords: Gram-negative bacteria; Oritavancin; Polymyxins; Synergy; Time-kill assay |
| المشرفين على المادة: | 0 (Anti-Bacterial Agents) 0 (Polymyxins) J2VZ07J96K (Polymyxin B) Z67X93HJG1 (Colistin) |
| تواريخ الأحداث: | Date Created: 20240704 Date Completed: 20240705 Latest Revision: 20240707 |
| رمز التحديث: | 20240707 |
| مُعرف محوري في PubMed: | PMC11225234 |
| DOI: | 10.1186/s12941-024-00720-4 |
| PMID: | 38965559 |
| قاعدة البيانات: | MEDLINE |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 1476-0711 |
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| DOI: | 10.1186/s12941-024-00720-4 |