دورية أكاديمية
Ex Vivo Evaluation of the Function of Hematopoietic Stem Cells in Toxicology of Metals.
| العنوان: | Ex Vivo Evaluation of the Function of Hematopoietic Stem Cells in Toxicology of Metals. |
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| المؤلفون: | Wu J; Experimental Center for Research, School of Public Health, Fudan University, Shanghai, China., Liu T; Experimental Center for Research, School of Public Health, Fudan University, Shanghai, China., Tang M; Experimental Center for Research, School of Public Health, Fudan University, Shanghai, China., Liu Y; Experimental Center for Research, School of Public Health, Fudan University, Shanghai, China., Wang W; Experimental Center for Research, School of Public Health, Fudan University, Shanghai, China., Wang C; Experimental Center for Research, School of Public Health, Fudan University, Shanghai, China., Ju Y; Experimental Center for Research, School of Public Health, Fudan University, Shanghai, China., Zhao Y; Experimental Center for Research, School of Public Health, Fudan University, Shanghai, China., Zhang Y; Experimental Center for Research, School of Public Health, Fudan University, Shanghai, China. |
| المصدر: | Current protocols [Curr Protoc] 2024 Jul; Vol. 4 (7), pp. e1038. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: John Wiley & Sons Country of Publication: United States NLM ID: 101773894 Publication Model: Print Cited Medium: Internet ISSN: 2691-1299 (Electronic) Linking ISSN: 26911299 NLM ISO Abbreviation: Curr Protoc Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Hoboken, NJ : John Wiley & Sons, [2021]- |
| مواضيع طبية MeSH: | Hematopoietic Stem Cells*/drug effects , Hematopoietic Stem Cells*/metabolism , Hematopoietic Stem Cells*/cytology, Animals ; Metals/toxicity ; Mice ; Humans ; Hematopoietic Stem Cell Transplantation ; Flow Cytometry/methods |
| مستخلص: | A variety of metals, e.g., lead (Pb), cadmium (Cd), and lithium (Li), are in the environment and are toxic to humans. Hematopoietic stem cells (HSCs) reside at the apex of hematopoiesis and are capable of generating all kinds of blood cells and self-renew to maintain the HSC pool. HSCs are sensitive to environmental stimuli. Metals may influence the function of HSCs by directly acting on HSCs or indirectly by affecting the surrounding microenvironment for HSCs in the bone marrow (BM) or niche, including cellular and extracellular components. Investigating the impact of direct and/or indirect actions of metals on HSCs contributes to the understanding of immunological and hematopoietic toxicology of metals. Treatment of HSCs with metals ex vivo, and the ensuing HSC transplantation assays, are useful for evaluating the impacts of the direct actions of metals on the function of HSCs. Investigating the mechanisms involved, given the rarity of HSCs, methods that require large numbers of cells are not suitable for signal screening; however, flow cytometry is a useful tool for signal screening HSCs. After targeting signaling pathways, interventions ex vivo and HSCs transplantation are required to confirm the roles of the signaling pathways in regulating the function of HSCs exposed to metals. Here, we describe protocols to evaluate the mechanisms of direct and indirect action of metals on HSCs. © 2024 Wiley Periodicals LLC. Basic Protocol 1: Identify the impact of a metal on the competence of HSCs Basic Protocol 2: Identify the impact of a metal on the lineage bias of HSC differentiation Basic Protocol 3: Screen the potential signaling molecules in HSCs during metal exposure Alternate Protocol 1: Ex vivo treatment with a metal on purified HSCs Alternate Protocol 2: Ex vivo intervention of the signaling pathway regulating the function of HSCs during metal exposure. (© 2024 Wiley Periodicals LLC.) |
| التعليقات: | Erratum in: Curr Protoc. 2024 Jul;4(7):e1115. doi: 10.1002/cpz1.1115. (PMID: 39040032) |
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| معلومات مُعتمدة: | 82173546 National Natural Science Foundation of China; GWVI-11.1-39 Shanghai 3-year Public Health Action Plan |
| فهرسة مساهمة: | Keywords: competence; ex vivo intervention; hematopoietic stem cells; lineage bias; metal; signal screening |
| المشرفين على المادة: | 0 (Metals) |
| تواريخ الأحداث: | Date Created: 20240705 Date Completed: 20240705 Latest Revision: 20240723 |
| رمز التحديث: | 20240723 |
| DOI: | 10.1002/cpz1.1038 |
| PMID: | 38967962 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 2691-1299 |
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| DOI: | 10.1002/cpz1.1038 |