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Glucose transporters and sodium glucose co-transporters cooperatively import glucose into energy-demanding organs in carcinogenic liver fluke Clonorchis sinensis.

التفاصيل البيبلوغرافية
العنوان: Glucose transporters and sodium glucose co-transporters cooperatively import glucose into energy-demanding organs in carcinogenic liver fluke Clonorchis sinensis.
المؤلفون: Dai F; Department of Parasitology, School of Biology and Basic Medical Sciences, MOE Key Laboratory of Geriatric Diseases and Immunology, Suzhou Key Laboratory of Pathogen Bioscience and Anti-infective Medicine, Suzhou Medical College, Soochow University, Suzhou, China.; Department of Medical Sciences, Chung-Ang University College of Medicine, Seoul, Republic of Korea., Lee SO; Department of Medical Sciences, Chung-Ang University College of Medicine, Seoul, Republic of Korea.; Department of Medical Zoology and Medical Research Center for Bioreaction to Reactive Oxygen Species and Biomedical Science Institute, School of Medicine, Graduate School, Kyung Hee University, Seoul, Republic of Korea., Song JH; Department of Pharmacology, Chung-Ang University College of Medicine, Seoul, Republic of Korea., Yoo WG; Department of Medical Sciences, Chung-Ang University College of Medicine, Seoul, Republic of Korea.; Laboratory of Veterinary Parasitology, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul, Republic of Korea., Shin EH; Department of Tropical Medicine and Parasitology, Seoul National University College of Medicine, and Institute of Endemic Diseases, Seoul, Republic of Korea.; Seoul National University Bundang Hospital, Seongnam, Republic of Korea., Bai X; Department of Medical Sciences, Chung-Ang University College of Medicine, Seoul, Republic of Korea.; Medical Research Center, Binzhou Medical University Hospital, Binzhou, China., Hong SJ; Department of Medical Sciences, Chung-Ang University College of Medicine, Seoul, Republic of Korea.; Center for Infectious Vectors and Diseases, Incheon National University, Incheon, Republic of Korea.
المصدر: PLoS neglected tropical diseases [PLoS Negl Trop Dis] 2024 Jul 05; Vol. 18 (7), pp. e0012315. Date of Electronic Publication: 2024 Jul 05 (Print Publication: 2024).
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Public Library of Science Country of Publication: United States NLM ID: 101291488 Publication Model: eCollection Cited Medium: Internet ISSN: 1935-2735 (Electronic) Linking ISSN: 19352727 NLM ISO Abbreviation: PLoS Negl Trop Dis Subsets: MEDLINE
أسماء مطبوعة: Original Publication: San Francisco, CA : Public Library of Science
مواضيع طبية MeSH: Clonorchis sinensis*/metabolism , Clonorchis sinensis*/genetics , Glucose*/metabolism , Sodium-Glucose Transport Proteins*/metabolism , Sodium-Glucose Transport Proteins*/genetics , Glucose Transport Proteins, Facilitative*/metabolism , Glucose Transport Proteins, Facilitative*/genetics, Animals ; Clonorchiasis/parasitology ; Biological Transport
مستخلص: Background: The liver fluke Clonorchis sinensis imports large amounts of glucose to generate energy and metabolic intermediates through glycolysis. We hypothesized that C. sinensis absorbs glucose through glucose transporters and identified four subtypes of glucose transporter (CsGTP) and one sodium glucose co-transporter (CsSGLT) in C. sinensis.
Methodology/principal Findings: Expressed sequence tags encoding CsGTPs were retrieved from the C. sinensis transcriptome database, and their full-length cDNA sequences were obtained by rapid amplification of cDNA ends (RACE). The tissue distribution of glucose transporters in C. sinensis adults was determined using immunohistochemical staining. Developmental expression was measured using RT-qPCR. The transport and distribution of glucose into living C. sinensis were monitored using confocal microscopy. Membrane topology and key functional residues of CsGTPs were homologous to their counterparts in animals and humans. CsGTP1, 2, and 4 were transcribed 2.4-5.5 times higher in the adults than metacercariae, while CsGTP3 was transcribed 2.1 times higher in the metacercariae than adults. CsSGLT transcription was 163.6 times higher in adults than in metacercariae. In adults, CsSGLT was most abundant in the tegument; CsGTP3 and CsSGLT were localized in the vitelline gland, uterine wall, eggs, mesenchymal tissue, and testes; CsGTP4 was found in sperm and mesenchymal tissue; and CsGTP1 was mainly in the sperm and testes. In C. sinensis adults, exogenous glucose is imported in a short time and is present mainly in the middle and posterior body, in which the somatic and reproductive organs are located. Of the exogenous glucose, 53.6% was imported through CsSGLT and 46.4% through CsGTPs. Exogenous glucose import was effectively inhibited by cytochalasin B and phlorizin.
Conclusions/significance: We propose that CsSGLT cooperates with CsGTPs to import exogenous glucose from the environmental bile, transport glucose across mesenchymal tissue cells, and finally supply energy-demanding organs in C. sinensis adults. Studies on glucose transporters may pave the way for the development of new anthelmintic drugs.
Competing Interests: The authors have declared that no competing interests exist.
(Copyright: © 2024 Dai et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
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المشرفين على المادة: IY9XDZ35W2 (Glucose)
0 (Sodium-Glucose Transport Proteins)
0 (Glucose Transport Proteins, Facilitative)
تواريخ الأحداث: Date Created: 20240705 Date Completed: 20240717 Latest Revision: 20240719
رمز التحديث: 20240719
مُعرف محوري في PubMed: PMC11253919
DOI: 10.1371/journal.pntd.0012315
PMID: 38968307
قاعدة البيانات: MEDLINE
الوصف
تدمد:1935-2735
DOI:10.1371/journal.pntd.0012315