دورية أكاديمية
Identification of circulating miRNA biomarkers in leptospirosis for early detection: A promising diagnostic approach.
| العنوان: | Identification of circulating miRNA biomarkers in leptospirosis for early detection: A promising diagnostic approach. |
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| المؤلفون: | Pinto GV; Nitte (Deemed to Be University), Department of Infectious Diseases and Microbial Genomics, Nitte University Centre for Science Education and Research, Deralakatte, Mangalore, 575018, Karnataka, India., Rai P; Nitte (Deemed to Be University), Department of Infectious Diseases and Microbial Genomics, Nitte University Centre for Science Education and Research, Deralakatte, Mangalore, 575018, Karnataka, India., Kabekkodu SP; Nitte (Deemed to Be University), Department of General Medicine, K.S. Hegde Medical Academy, Deralakatte, Mangalore, 575018, Karnataka, India., Karunasagar I; Nitte (Deemed to Be University), Department of Infectious Diseases and Microbial Genomics, Nitte University Centre for Science Education and Research, Deralakatte, Mangalore, 575018, Karnataka, India., Kumar BK; Nitte (Deemed to Be University), Department of Infectious Diseases and Microbial Genomics, Nitte University Centre for Science Education and Research, Deralakatte, Mangalore, 575018, Karnataka, India. Electronic address: krishnakumarb@nitte.edu.in. |
| المصدر: | Microbial pathogenesis [Microb Pathog] 2024 Aug; Vol. 193, pp. 106781. Date of Electronic Publication: 2024 Jul 03. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Academic Press Country of Publication: England NLM ID: 8606191 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1096-1208 (Electronic) Linking ISSN: 08824010 NLM ISO Abbreviation: Microb Pathog Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: London ; Orlando : Academic Press, c1986- |
| مواضيع طبية MeSH: | Leptospirosis*/diagnosis , Leptospirosis*/blood , Biomarkers*/blood , Early Diagnosis* , Circulating MicroRNA*/blood , Circulating MicroRNA*/genetics, Humans ; MicroRNAs/blood ; Real-Time Polymerase Chain Reaction ; Adult ; Male ; Gene Expression Profiling ; Leptospira/genetics ; Leptospira/isolation & purification ; Leptospira/immunology ; Female |
| مستخلص: | Leptospirosis is a zoonotic disease of global significance, contributing to morbidity and mortality worldwide. It is endemic to tropical regions, with outbreaks during monsoons. The disease manifestations are similar to that of other febrile illness such as dengue, malaria hence often misdiagnosed and underreported. The zoonoses if undetected, progresses to cause severe life-threatening complications also known as Weil's disease. Routine diagnostic tests are based on the detection of antibodies in patient serum and are not accurate during the initial phase of the infection. Therefore, it is necessary to detect novel biomarkers that can be used in early detection of leptospirosis. Circulating miRNAs are known to be promising biomarkers for various diseases including cancer, tuberculosis, influenza; hence in this study the potential of miRNAs as biomarkers for leptospirosis was evaluated. A total of 30 leptospirosis cases were screened for the differential expression of 10 miRNA by RT-qPCR assay. The differential expression was calculated by relative quantification using healthy individuals as controls. Among the 10 miRNA,3 miRNA, miR-28-5p, miR-302c-3p and miR-302a-3p were reported to exhibit a significant trend of upregulation. Further their role in immune pathways and biological processes was investigated by KEGG analysis and Gene Ontology. The 3 miRNAs were observed to target various immune response pathways, thus confirming their role in host immune response. Based on the results obtained in this study, miR-28-5p, miR-302c-3p and miR-302a-3p can be considered as potential biomarkers for the detection of leptospirosis. Competing Interests: Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Gillaine Vail Pinto, Praveen Rai, Shama Prakash Kabekkodu, Indrani Karunasagar, Ballamoole Krishna Kumar has patent pending to Intellectual Property India. If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2024 Elsevier Ltd. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Biomarker; Diagnostics; Leptospirosis; RT-qPCR; Zoonotic disease; miRNA |
| المشرفين على المادة: | 0 (Biomarkers) 0 (Circulating MicroRNA) 0 (MicroRNAs) |
| تواريخ الأحداث: | Date Created: 20240705 Date Completed: 20240722 Latest Revision: 20240722 |
| رمز التحديث: | 20240723 |
| DOI: | 10.1016/j.micpath.2024.106781 |
| PMID: | 38969187 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1096-1208 |
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| DOI: | 10.1016/j.micpath.2024.106781 |