دورية أكاديمية
The drainome: longitudinal metagenomic characterisation of wastewater from hospital ward sinks to characterize the microbiome and resistome and assess the effects of decontamination interventions.
| العنوان: | The drainome: longitudinal metagenomic characterisation of wastewater from hospital ward sinks to characterize the microbiome and resistome and assess the effects of decontamination interventions. |
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| المؤلفون: | Snell LB; Department of Infectious Diseases, King's College, London, UK; Directorate of Infection, Guy's and St Thomas' NHS Foundation Trust, London, UK. Electronic address: luke.snell@kcl.ac.uk., Prossomariti D; Directorate of Infection, Guy's and St Thomas' NHS Foundation Trust, London, UK., Alcolea-Medina A; Department of Infectious Diseases, King's College, London, UK; Infection Sciences, Synnovis Analytics LLP, London, UK., Sasson M; Directorate of Infection, Guy's and St Thomas' NHS Foundation Trust, London, UK., Dibbens M; Directorate of Infection, Guy's and St Thomas' NHS Foundation Trust, London, UK., Al-Yaakoubi N; Directorate of Infection, Guy's and St Thomas' NHS Foundation Trust, London, UK., Humayun G; Directorate of Infection, Guy's and St Thomas' NHS Foundation Trust, London, UK., Charalampous T; Department of Infectious Diseases, King's College, London, UK., Alder C; Directorate of Infection, Guy's and St Thomas' NHS Foundation Trust, London, UK., Ward D; Directorate of Infection, Guy's and St Thomas' NHS Foundation Trust, London, UK., Maldonado-Barrueco A; Directorate of Infection, Guy's and St Thomas' NHS Foundation Trust, London, UK., Abadioru O; Directorate of Infection, Guy's and St Thomas' NHS Foundation Trust, London, UK., Batra R; Directorate of Infection, Guy's and St Thomas' NHS Foundation Trust, London, UK., Nebbia G; Department of Infectious Diseases, King's College, London, UK; Directorate of Infection, Guy's and St Thomas' NHS Foundation Trust, London, UK., Otter JA; Directorate of Infection, Guy's and St Thomas' NHS Foundation Trust, London, UK., Edgeworth JD; Department of Infectious Diseases, King's College, London, UK; Directorate of Infection, Guy's and St Thomas' NHS Foundation Trust, London, UK., Goldenberg SD; Department of Infectious Diseases, King's College, London, UK; Directorate of Infection, Guy's and St Thomas' NHS Foundation Trust, London, UK. |
| المصدر: | The Journal of hospital infection [J Hosp Infect] 2024 Jul 03. Date of Electronic Publication: 2024 Jul 03. |
| Publication Model: | Ahead of Print |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: W.B. Saunders For The Hospital Infection Society Country of Publication: England NLM ID: 8007166 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1532-2939 (Electronic) Linking ISSN: 01956701 NLM ISO Abbreviation: J Hosp Infect Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: London : W.B. Saunders For The Hospital Infection Society Original Publication: New York, Academic Press. |
| مستخلص: | Background: Hospital drains and water interfaces are implicated in nosocomial transmission of pathogens. Metagenomics can assess the microbial composition and presence of antimicrobial resistance genes in drains ('the drainome') but studies applying these methods longitudinally and to assess infection control interventions are lacking. Aim: Apply long-read metagenomics coupled with microbiological measurements to investigate the drainome and assess the effects of a peracetic acid-containing decontamination product. Methods: 12-week study in three phases: a baseline phase, an intervention phase of enhanced decontamination with peracetic acid, and a post-intervention phase. Five hospital sink drains on an intensive care unit were sampled twice weekly. Each sample had 1) measurement of total viable count (TVC), 2) metagenomic analyses including i) taxonomic classification of bacteria and fungi ii) antibiotic resistance gene detection iii) plasmid identification, and 3) immunochromatographic detection of antimicrobial residues. Findings: Overall TVCs remain unchanged in the intervention phase (+386 CFU/mL, SE 705, p=0.59). There was a small but significant increase in the microbial diversity in the intervention phase (-0.07 in Simpson's index, SE 0.03, p=0.007), which was not sustained post-intervention (-0.05, SE 0.03, p=0.08). The intervention was associated with increased relative abundance of the Pseudomonas genus (18.3% to 40.5% [+22.2%], SE 5.7%, p<0.001). Extended spectrum beta-lactamases were found in all samples, with NDM-carbapenemase found in 3 drains in 6 samples. Antimicrobial residues were detected in a large proportion of samples (31/115, 27%), suggesting use of sinks for non-handwashing activities. Conclusions: Metagenomics and other measurements can measure the composition of the drainome and assess the effectiveness of decontamination interventions. (Copyright © 2024 The Author(s). Published by Elsevier Ltd.. All rights reserved.) |
| فهرسة مساهمة: | Keywords: drain microbiome; drainome; wastewater surveillance |
| تواريخ الأحداث: | Date Created: 20240705 Latest Revision: 20240705 |
| رمز التحديث: | 20240706 |
| DOI: | 10.1016/j.jhin.2024.06.005 |
| PMID: | 38969209 |
| قاعدة البيانات: | MEDLINE |
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Full Text Finder | تدمد: | 1532-2939 |
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| DOI: | 10.1016/j.jhin.2024.06.005 |