دورية أكاديمية

Characteristic changes in blood routine and peripheral blood lymphocyte subpopulations in recipients of different types of rejection.

التفاصيل البيبلوغرافية
العنوان: Characteristic changes in blood routine and peripheral blood lymphocyte subpopulations in recipients of different types of rejection.
عنوان ترانسليتريتد: 不同类型排斥反应受者血常规及外周血淋巴细胞亚群特征性变化.
المؤلفون: Luo S; Department of Kidney Transplantation, Second Xiangya Hospital, Central South University, Changsha 410011. 853030336@qq.com.; Clinical Research Center for Organ Transplantation in Hunan Province, Second Xiangya Hospital, Central South University, Changsha 410011. 853030336@qq.com., Nie M; Department of Kidney Transplantation, Second Xiangya Hospital, Central South University, Changsha 410011.; Clinical Research Center for Organ Transplantation in Hunan Province, Second Xiangya Hospital, Central South University, Changsha 410011., Song L; Department of Kidney Transplantation, Second Xiangya Hospital, Central South University, Changsha 410011.; Clinical Research Center for Organ Transplantation in Hunan Province, Second Xiangya Hospital, Central South University, Changsha 410011., Xie Y; Department of Kidney Transplantation, Second Xiangya Hospital, Central South University, Changsha 410011.; Clinical Research Center for Organ Transplantation in Hunan Province, Second Xiangya Hospital, Central South University, Changsha 410011., Zhong M; Department of Kidney Transplantation, Second Xiangya Hospital, Central South University, Changsha 410011.; Clinical Research Center for Organ Transplantation in Hunan Province, Second Xiangya Hospital, Central South University, Changsha 410011., Tan S; Department of Kidney Transplantation, Second Xiangya Hospital, Central South University, Changsha 410011.; Clinical Research Center for Organ Transplantation in Hunan Province, Second Xiangya Hospital, Central South University, Changsha 410011.; Department of Urology, Institute of Urology Transplantation, Second Hospital, University of South China, Hengyang Hunan 421001, China., An R; Department of Kidney Transplantation, Second Xiangya Hospital, Central South University, Changsha 410011.; Clinical Research Center for Organ Transplantation in Hunan Province, Second Xiangya Hospital, Central South University, Changsha 410011., Li P; Department of Kidney Transplantation, Second Xiangya Hospital, Central South University, Changsha 410011.; Clinical Research Center for Organ Transplantation in Hunan Province, Second Xiangya Hospital, Central South University, Changsha 410011., Tan L; Department of Kidney Transplantation, Second Xiangya Hospital, Central South University, Changsha 410011. tan1802@csu.edu.cn.; Clinical Research Center for Organ Transplantation in Hunan Province, Second Xiangya Hospital, Central South University, Changsha 410011. tan1802@csu.edu.cn., Xie X; Department of Kidney Transplantation, Second Xiangya Hospital, Central South University, Changsha 410011. xiexubiao@csu.edu.cn.; Clinical Research Center for Organ Transplantation in Hunan Province, Second Xiangya Hospital, Central South University, Changsha 410011. xiexubiao@csu.edu.cn.
المصدر: Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences [Zhong Nan Da Xue Xue Bao Yi Xue Ban] 2024 Mar 28; Vol. 49 (3), pp. 417-425.
نوع المنشور: Journal Article
اللغة: English; Chinese
بيانات الدورية: Publisher: Zhong nan da xue xue bao (yi xue ban)" bian ji bu Country of Publication: China NLM ID: 101230586 Publication Model: Print Cited Medium: Print ISSN: 1672-7347 (Print) Linking ISSN: 16727347 NLM ISO Abbreviation: Zhong Nan Da Xue Xue Bao Yi Xue Ban Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Changsha Shi : "Zhong nan da xue xue bao (yi xue ban)" bian ji bu, 2004-
مواضيع طبية MeSH: Graft Rejection*/blood , Graft Rejection*/diagnosis , Graft Rejection*/immunology , Kidney Transplantation*, Humans ; Retrospective Studies ; Female ; Male ; Lymphocyte Subsets/immunology ; Adult ; Middle Aged ; T-Lymphocytes/immunology
مستخلص: Objectives: Rejection remains the most important factor limiting the survival of transplanted kidneys. Although a pathological biopsy of the transplanted kidney is the gold standard for diagnosing rejection, its limitations prevent it from being used as a routine monitoring method. Recently, peripheral blood lymphocyte subpopulation testing has become an important means of assessing the body's immune system, however, its application value and strategy in the field of kidney transplantation need further exploration. Additionally, the development and utilization of routine test parameters are also important methods for exploring diagnostic strategies and predictive models for kidney transplant diseases. This study aims to explore the correlation between peripheral blood lymphocyte subpopulations and T cell-mediated rejection (TCMR) and antibody-mediated rejection (ABMR), as well as their diagnostic value, in conjunction with routine blood tests.
Methods: A total of 154 kidney transplant recipients, who met the inclusion and exclusion criteria and were treated at the Second Xiangya Hospital of Central South University from January to December, 2021, were selected as the study subjects. They were assigned into a stable group, a TCMR group, and an ABMR group, based on the occurrence and type of rejection. The basic and clinical data of these recipients were retrospectively analyzed and compared among the 3 groups. The transplant kidney function, routine blood tests, and peripheral blood lymphocyte subpopulation data of the TCMR group and the ABMR group before rejection treatment were compared with those of the stable group.
Results: The stable, TCMR group, and ABMR group showed no statistically significant differences in immunosuppressive maintenance regimens or sources of transplanted kidneys (all P >0.05). However, the post-transplant duration was significantly longer in the ABMR group compared with the stable group ( P <0.001) and the TCMR group ( P <0.05). Regarding kidney function, serum creatinine levels in the ABMR group were higher than in the stable group and the TCMR group (both P <0.01), with the TCMR group also showing higher levels than the stable group ( P <0.01). Both TCMR and ABMR groups had significantly higher blood urea nitrogen levels than the stable group ( P <0.01), with no statistically significant difference between TCMR and ABMR groups ( P >0.05). The estimated glomerular filtration rate (eGFR) was lower in both TCMR and ABMR groups compared with the stable group (both P <0.01). In routine blood tests, the ABMR group had lower hemoglobin, red blood cell count, and platelet count than the stable group (all P <0.05). The TCMR group had higher neutrophil percentage ( P <0.05) and count ( P <0.05) than the stable group, and the ABMR group had a higher neutrophil percentage than the stable group ( P <0.05). The eosinophil percentage and count in the TCMR group were lower than in the stable and ABMR groups (all P <0.05). Both TCMR and ABMR groups had lower basophil percentage and count, as well as lower lymphocyte percentage and count, compared with the stable group (all P <0.05). There were no significant differences in monocyte percentage and count among the 3 groups (all P >0.05). In lymphocyte subpopulations, the TCMR and ABMR groups had lower counts of CD45 + cells and T cells compared with the stable group (all P <0.05). The TCMR group also had lower counts of CD4 + T cells, NK cells, and B cells than the stable group (all P <0.05). There were no significant differences in the T cell percentage, CD4 + T cell percentage, CD8 + T cell percentage and their counts, CD4 + /CD8 + T cell ratio, NK cell percentage, and B cell percentage among the stable, TCMR, and ABMR groups (all P >0.05).
Conclusions: The occurrence of rejection leads to impaired transplant kidney function, accompanied by characteristic changes in some parameters of routine blood tests and peripheral blood lymphocyte subpopulations in kidney transplant recipients. The different characteristics of changes in some parameters of routine blood tests and peripheral blood lymphocyte subpopulations during TCMR and ABMR may help predict and diagnose rejection and differentiate between TCMR and ABMR.
References: Clin Exp Nephrol. 2019 Aug;23(8):1066-1075. (PMID: 31020441)
Clin Rheumatol. 2018 Feb;37(2):459-465. (PMID: 28988406)
Nephrol Dial Transplant. 2023 Feb 28;38(3):764-777. (PMID: 36073758)
J Am Soc Nephrol. 2021 Jun 1;32(6):1513-1526. (PMID: 33883251)
Nephrol Dial Transplant. 2019 May 1;34(5):750-756. (PMID: 31009949)
Am J Transplant. 2015 Mar;15(3):806-14. (PMID: 25656947)
Clin Exp Immunol. 2019 Jun;196(3):403-414. (PMID: 30712266)
Am J Transplant. 2017 Jan;17(1):28-41. (PMID: 27862883)
Front Immunol. 2022 Mar 31;13:852079. (PMID: 35432350)
Trends Immunol. 2023 Aug;44(8):577-584. (PMID: 37402600)
Transplant Rev (Orlando). 2019 Apr;33(2):87-98. (PMID: 30551846)
Biomed Res Int. 2021 Feb 09;2021:6627909. (PMID: 33628795)
Transplant Direct. 2019 Aug 08;5(9):e481. (PMID: 31579809)
Ann Transplant. 2018 Jul 10;23:467-474. (PMID: 29987271)
J Am Soc Nephrol. 2015 Jul;26(7):1711-20. (PMID: 25377077)
Am J Transplant. 2018 Feb;18(2):293-307. (PMID: 29243394)
J Am Soc Nephrol. 2015 Apr;26(4):855-63. (PMID: 25145937)
Clin Chim Acta. 2021 Dec;523:247-259. (PMID: 34626603)
Am J Transplant. 2020 Jun;20 Suppl 4:23-32. (PMID: 32538534)
Transplantation. 1986 Jun;41(6):709-12. (PMID: 3520986)
J Nephrol. 2017 Apr;30(2):187-200. (PMID: 27245689)
Transplantation. 2013 Oct 27;96(8):717-25. (PMID: 23896555)
J Am Soc Nephrol. 2020 Oct;31(10):2457-2474. (PMID: 32723838)
Diagnostics (Basel). 2021 Apr 01;11(4):. (PMID: 33916199)
PLoS One. 2020 Jun 12;15(6):e0234323. (PMID: 32530943)
Ann Intern Med. 2003 Jul 15;139(2):137-47. (PMID: 12859163)
EBioMedicine. 2021 Nov;73:103645. (PMID: 34688031)
Trends Mol Med. 2022 Mar;28(3):237-250. (PMID: 35093288)
Int J Mol Sci. 2023 Mar 09;24(6):. (PMID: 36982359)
معلومات مُعتمدة: 2021JJ40864 and 2024JJ2088 the Natural Science Foundation of Hunan Province
فهرسة مساهمة: Keywords: T cell-mediated rejection; antibody-mediated rejection; blood routine; kidney transplantation; lymphocyte subpopulations
Local Abstract: [Publisher, Chinese] 目的 : 排斥反应一直是限制移植肾存活的最重要因素。移植肾病理活检是排斥反应诊断的金标准,但因其局限性并不能作为常规监测手段。近来,外周血淋巴细胞亚群检测已成为评估机体免疫系统的重要手段,然而其在肾移植领域的应用价值和策略有待探究;此外,常规检验参数的新开发利用也是探索肾移植疾病诊断策略和预测模型的重要手段。本研究旨在结合血常规,探究外周血淋巴细胞亚群与T细胞介导的排斥反应(T cell-mediated rejection,TCMR)和抗体介导的排斥反应(antibody-mediated rejection,ABMR)的相关性及其协助诊断价值。 方法 : 回顾性分析2021年1至12月就诊于中南大学湘雅二医院且符合纳入标准的154例肾移植受者的基本资料以及临床资料。根据排斥反应是否发生及其类型分为稳定组、TCMR组和ABMR组。比较3组的基本资料,并将TCMR组和ABMR组排斥反应治疗前的移植肾功能、血常规和外周血淋巴细胞亚群数据与稳定组进行比较。 结果 : 稳定组、TCMR组和ABMR组在免疫抑制维持方案、移植肾来源等方面差异均无统计学意义(均 P >0.05),但ABMR组的肾移植术后时间显著长于稳定组( P <0.001)和TCMR组( P <0.05)。在移植肾功能方面,ABMR组的血肌酐值均高于稳定组和TCMR组(均 P <0.01),并且TCMR组也高于稳定组( P <0.01);TCMR组和ABMR组的尿素氮均显著高于稳定组(均 P< 0.01),而TCMR组和ABMR组之间差异无统计学意义( P >0.05);TCMR组和ABMR组的估算肾小球滤过率(estimated glomerular filtration rate,eGFR)均低于稳定组(均 P< 0.01)。在血常规方面:ABMR组的血红蛋白、红细胞数和血小板数均低于稳定组(均 P< 0.05);TCMR组的中性粒细胞比例和数量均高于稳定组(均 P <0.05),ABMR组的中性粒细胞比例高于稳定组( P< 0.05);TCMR组的嗜酸性粒细胞比例和数量均低于稳定组和ABMR组(均 P <0.05);TCMR组和ABMR组的嗜碱性粒细胞比例和数量、淋巴细胞比例和数量均低于稳定组(均 P <0.05);稳定组、TCMR组和ABMR组的单核细胞比例和数量差异均无统计学意义(均 P> 0.05)。在淋巴细胞亚群方面:TCMR组和ABMR组的CD45 + 细胞和T细胞数均低于稳定组(均 P< 0.05);TCMR组的CD4 + T细胞数、NK细胞数和B细胞数均低于稳定组(均 P <0.05);稳定组、TCMR组和ABMR组在T细胞比例、CD4 + T细胞比例、CD8 + T细胞比例和数量、CD4 + /CD8 + T细胞比值、NK细胞比例、B细胞比例方面差异均无统计学意义(均 P> 0.05)。 结论 : 排斥反应的发生导致移植肾功能受损,同时会伴有肾移植受者血常规、外周血淋巴细胞亚群部分指标的特征性变化。TCMR和ABMR发生时血常规、外周血淋巴细胞亚群部分指标改变特点的不同可能有助于预测和诊断排斥反应以及二者的鉴别。.
تواريخ الأحداث: Date Created: 20240706 Date Completed: 20240706 Latest Revision: 20240708
رمز التحديث: 20240708
مُعرف محوري في PubMed: PMC11208394
DOI: 10.11817/j.issn.1672-7347.2024.230543
PMID: 38970516
قاعدة البيانات: MEDLINE
الوصف
تدمد:1672-7347
DOI:10.11817/j.issn.1672-7347.2024.230543