دورية أكاديمية
Effects of photobiomodulation on colon cancer cell line HT29 according to mitochondria.
| العنوان: | Effects of photobiomodulation on colon cancer cell line HT29 according to mitochondria. |
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| المؤلفون: | Seo KJ; Department of Pathology, Uijongbu St. Mary's Hospital, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea., Yoon JH; Department of Pathology, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea., Chung BY; Department of Internal Medicine, Uijongbu St. Mary's Hospital, The Catholic University of Korea, Seoul, Republic of Korea., Lee HK; Department of Laboratory Medicine, Uijongbu St. Mary's Hospital, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea., Park WS; Department of Pathology, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea. Electronic address: wonsang@catholic.ac.kr., Chae HS; Department of Internal Medicine, Uijongbu St. Mary's Hospital, The Catholic University of Korea, Seoul, Republic of Korea. Electronic address: chs@catholic.ac.kr. |
| المصدر: | Journal of photochemistry and photobiology. B, Biology [J Photochem Photobiol B] 2024 Aug; Vol. 257, pp. 112966. Date of Electronic Publication: 2024 Jun 27. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Sequoia Country of Publication: Switzerland NLM ID: 8804966 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-2682 (Electronic) Linking ISSN: 10111344 NLM ISO Abbreviation: J Photochem Photobiol B Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Lausanne : Elsevier Sequoia, 1987- |
| مواضيع طبية MeSH: | Mitochondria*/metabolism , Mitochondria*/radiation effects , Cell Proliferation*/radiation effects , Mice, Nude* , Reactive Oxygen Species*/metabolism , Colonic Neoplasms*/pathology , Colonic Neoplasms*/radiotherapy , Colonic Neoplasms*/metabolism , Membrane Potential, Mitochondrial*/radiation effects, Humans ; HT29 Cells ; Animals ; Mice ; Low-Level Light Therapy ; Cell Movement/radiation effects ; Cyclin B1/metabolism ; Mitochondrial Dynamics/radiation effects ; Cyclin D1/metabolism ; Proto-Oncogene Proteins c-akt/metabolism |
| مستخلص: | Background/aim: Although photobiomodulation therapy (PBMt) is available to alleviate post-operative side effects of malignant diseases, its application is still controversial due to some potential of cancer recurrence and occurrence of a secondary malignancy. We investigated effect of PBMt on mitochondrial function in HT29 colon cancer cells. Methods: HT29 cell proliferation was determined with MTT assay after PBMt. Immunofluorescent staining was performed to determine mitochondrial biogenesis and reactive oxygen species (ROS). Mitochondrial membrane potential was measured with Mitotracker. Western blotting was executed to determine expression of fission, fusion, UCP2, and cyclin B1 and D1 proteins. In vivo study was performed by subcutaneously inoculating cancer cells into nude mice and immunohistochemistry was done to determine expression of FIS1, MFN2, UCP2, and p-AKT. Results: The proliferation and migration of HT29 cells reached maximum with PBMt (670 nm, light emitting diode, LED) at 2.0 J/cm 2 compared to control (P < 0.05) with more expression of cyclin B1 and cyclin D1 (P < 0.05). Immunofluorescent staining showed that ROS and mitochondrial membrane potential were enhanced after PBMt compared to control. ATP synthesis of mitochondria was also higher in the PBMt group than in the control (P < 0.05). Expression levels of fission and fusion proteins were significantly increased in the PBMt group than in the control (P < 0.05). Electron microscopy revealed that the percentage of mitochondria showing fission was not significantly different between the two groups. Oncometabolites including D-2-hydoxyglutamate in the supernatant of cell culture were higher in the PBMt group than in the control with increased UCP2 expression (P < 0.05). Both tumor size and weight of xenograft in nude mice model were bigger and heavier in the PBMt group than in the control (P < 0.05). Immunohistologically, mitochondrial biogenesis proteins UCP2 and p-AKT in xenograft of nude mice were expressed more in the PBMt group than in the control (P < 0.05). Conclusions: Treatment with PBM using red light LED may induce proliferation and progression of HT29 cancer cells by increasing mitochondrial activity and fission. Competing Interests: Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Hiun Suk Chae reports financial support was provided by National Research Foundation of Korea. Hiun Suk Chae reports a relationship with National Research Foundation of Korea that includes: funding grants. If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2024 Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Colon cancer; Mitochondrial dynamics; Oncometabolite; Photobiomodulation |
| المشرفين على المادة: | 0 (Reactive Oxygen Species) 0 (Cyclin B1) 136601-57-5 (Cyclin D1) EC 2.7.11.1 (Proto-Oncogene Proteins c-akt) |
| تواريخ الأحداث: | Date Created: 20240706 Date Completed: 20240714 Latest Revision: 20240714 |
| رمز التحديث: | 20240715 |
| DOI: | 10.1016/j.jphotobiol.2024.112966 |
| PMID: | 38970968 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1873-2682 |
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| DOI: | 10.1016/j.jphotobiol.2024.112966 |