دورية أكاديمية
The inhibitory effect of swine TAB1 on the replication of pseudorabies virus.
| العنوان: | The inhibitory effect of swine TAB1 on the replication of pseudorabies virus. |
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| المؤلفون: | Xia X; Shandong Key Laboratory of Animal Disease Control and Breeding/Key Laboratory of Livestock and Poultry Multi-omics of MARA, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Jinan, China; College of Life Science, Shandong Normal University, Jinan, China., Wu X; Shandong Key Laboratory of Animal Disease Control and Breeding/Key Laboratory of Livestock and Poultry Multi-omics of MARA, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Jinan, China., Jiang D; Shandong Key Laboratory of Animal Disease Control and Breeding/Key Laboratory of Livestock and Poultry Multi-omics of MARA, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Jinan, China., Hu Y; Shandong Key Laboratory of Animal Disease Control and Breeding/Key Laboratory of Livestock and Poultry Multi-omics of MARA, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Jinan, China., Cong X; Shandong Key Laboratory of Animal Disease Control and Breeding/Key Laboratory of Livestock and Poultry Multi-omics of MARA, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Jinan, China., Li J; Shandong Key Laboratory of Animal Disease Control and Breeding/Key Laboratory of Livestock and Poultry Multi-omics of MARA, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Jinan, China., Dai M; College of Life Science, Shandong Normal University, Jinan, China., Du Y; Shandong Key Laboratory of Animal Disease Control and Breeding/Key Laboratory of Livestock and Poultry Multi-omics of MARA, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Jinan, China; College of Life Science, Shandong Normal University, Jinan, China. Electronic address: duyijun0916@163.com., Qi J; Shandong Key Laboratory of Animal Disease Control and Breeding/Key Laboratory of Livestock and Poultry Multi-omics of MARA, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Jinan, China; College of Life Science, Shandong Normal University, Jinan, China. Electronic address: qj-happy2008@163.com. |
| المصدر: | Veterinary microbiology [Vet Microbiol] 2024 Sep; Vol. 296, pp. 110172. Date of Electronic Publication: 2024 Jul 02. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Scientific Pub. Co Country of Publication: Netherlands NLM ID: 7705469 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-2542 (Electronic) Linking ISSN: 03781135 NLM ISO Abbreviation: Vet Microbiol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Amsterdam, Elsevier Scientific Pub. Co. |
| مواضيع طبية MeSH: | Herpesvirus 1, Suid*/genetics , Herpesvirus 1, Suid*/physiology , Virus Replication* , Pseudorabies*/virology, Animals ; Swine ; Swine Diseases/virology ; Adaptor Proteins, Signal Transducing/genetics ; Adaptor Proteins, Signal Transducing/metabolism ; Cell Line ; CRISPR-Cas Systems ; Interferon-beta/genetics ; Interferon-beta/metabolism |
| مستخلص: | TAK1-binding protein 1 (TAB1) assembles with TAK1 through its C-terminal domain, leading to the self-phosphorylation and activation of TAK1, which plays an important role in the activation of NF-κB and MAPK signaling pathway. Pseudorabies virus (PRV) is the pathogen of Pseudorabies (PR), which belongs to the Alphaherpesvirus subfamily and causes serious economic losses to the global pig industry. However, the impact of swine TAB1 (sTAB1) on PRV infection has not been reported. In this study, evidence from virus DNA copies, virus titer and western blotting confirmed that sTAB1 could inhibit PRV replication and knockout of sTAB1 by CRISPR-Cas9 gene editing system could promote PRV replication. Further mechanistic studies by real-time PCR and luciferase reporter gene assay demonstrated that sTAB1 could enhance the production of inflammatory factors and chemokines, IFN-β transcription level and IFN-β promoter activity after PRV infection. In summary, we clarify the underlying mechanism of sTAB1 in inhibiting PRV replication for the first time, which provides a new idea for preventing PRV infection and lays a foundation for PRV vaccine development. Competing Interests: Declaration of Competing Interest The authors declare that there are no conflicts of interest. (Copyright © 2024 Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Chemokines; IFN-β; Inflammatory factors; Pseudorabies virus; STAB1 |
| المشرفين على المادة: | 0 (Adaptor Proteins, Signal Transducing) 77238-31-4 (Interferon-beta) |
| تواريخ الأحداث: | Date Created: 20240706 Date Completed: 20240806 Latest Revision: 20240806 |
| رمز التحديث: | 20240807 |
| DOI: | 10.1016/j.vetmic.2024.110172 |
| PMID: | 38971118 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1873-2542 |
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| DOI: | 10.1016/j.vetmic.2024.110172 |