دورية أكاديمية
[Recombinant Schistosoma japonicum cystatin alleviates acute liver injury in mice by inhibiting endoplasmic reticulum stress, inflammation and hepatocyte apoptosis].
| العنوان: | [Recombinant Schistosoma japonicum cystatin alleviates acute liver injury in mice by inhibiting endoplasmic reticulum stress, inflammation and hepatocyte apoptosis]. |
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| المؤلفون: | Lu L; Department of Pathology and Physiology, Shanxi Medical University, Taiyuan 030002, China., Yang X; Department of Human Parasitology, Anhui Provincial Key Laboratory of Infection and Immunology, Bengbu Medical University, Bengbu 233030, China., Zhang H; Department of Medical Laboratory, Hebi Vocational and Technical College, Hebi 458030, China., Liang Y; Department of Pathology and Physiology, Shanxi Medical University, Taiyuan 030002, China., Shi X; Department of Endoscopy, Taiyuan Third People's Hospital, Taiyuan 030012, China., Zhou X; Department of Pathology and Physiology, Shanxi Medical University, Taiyuan 030002, China. |
| المصدر: | Nan fang yi ke da xue xue bao = Journal of Southern Medical University [Nan Fang Yi Ke Da Xue Xue Bao] 2024 Jun 20; Vol. 44 (6), pp. 1126-1134. |
| نوع المنشور: | English Abstract; Journal Article |
| اللغة: | Chinese |
| بيانات الدورية: | Publisher: Nanfang yi ke da xue xue bao bian ji bu Country of Publication: China NLM ID: 101266132 Publication Model: Print Cited Medium: Print ISSN: 1673-4254 (Print) Linking ISSN: 16734254 NLM ISO Abbreviation: Nan Fang Yi Ke Da Xue Xue Bao Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Guangzhou : Nanfang yi ke da xue xue bao bian ji bu, 2005- |
| مواضيع طبية MeSH: | Schistosoma japonicum* , Endoplasmic Reticulum Chaperone BiP* , Endoplasmic Reticulum Stress*/drug effects , Apoptosis*/drug effects , Hepatocytes*/metabolism , Hepatocytes*/drug effects , Mice, Inbred C57BL* , Inflammation* , Cystatins*/pharmacology, Animals ; Mice ; Male ; Liver/pathology ; Liver/metabolism ; Lipopolysaccharides ; Tumor Necrosis Factor-alpha/metabolism ; Interleukin-6/metabolism ; Recombinant Proteins/pharmacology ; Proto-Oncogene Proteins c-bcl-2/metabolism ; bcl-2-Associated X Protein/metabolism ; Antigens, Differentiation, Myelomonocytic/metabolism ; Galactosamine ; Antigens, CD/metabolism ; Chemical and Drug Induced Liver Injury/drug therapy ; CD68 Molecule |
| مستخلص: | Objective: To investigate the protective effect of recombinant Schistosoma japonicum cystatin (rSj-Cys) against acute liver injury induced by lipopolysaccharide (LPS) and D-GalN in mice. Methods: Adult male C57BL/6J mice with or without LPS/D-GaIN-induced acute liver injury were given intraperitoneal injections of rSj-Cys or PBS 30 min after modeling ( n =18), and serum and liver tissues samples were collected from 8 mice in each group 6 h after modeling. The survival of the remaining 10 mice in each group within 24 h was observed. Serum levels of ALT, AST, TNF-α and IL-6 of the mice were measured, and liver pathologies was observed with HE staining. The hepatic expressions of macrophage marker CD68, Bax, Bcl-2 and endoplasmic reticulum stress (ERS)-related proteins were detected using immunohistochemistry or immunoblotting, and TUNEL staining was used to detect hepatocyte apoptosis. Results: The survival rates of PBS- and rSj-Cys-treated mouse models of acute liver injury were 30% and 80% at 12 h and were 10% and 60% at 24 h after modeling, respectively; no death occurred in the two control groups within 24 h. The mouse models showed significantly increased serum levels of AST, ALT, IL-6 and TNF-α and serious liver pathologies with increased hepatic expressions of CD68 and Bax, lowered expression of Bcl-2, increased hepatocyte apoptosis, and up-regulated expressions of ERS-related signaling pathway proteins GRP78, CHOP and NF-κB p-p65. Treatment of the mouse models significantly lowered the levels of AST, ALT, IL-6 and TNF-α, alleviated liver pathologies, reduced hepatic expressions of CD68, Bax, GRP78, CHOP and NF-κB p-p65, and enhanced the expression of Bcl-2. In the normal control mice, rSj-Cys injection did not produce any significant changes in these parameters compared with PBS. Conclusion: rSj-Cys alleviates LPS/D-GalN-induced acute liver injury in mice by suppressing ERS, attenuating inflammation and inhibiting hepatocyte apoptosis. |
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| فهرسة مساهمة: | Keywords: Schistosoma japonicum cystatin; acute liver injury; apoptosis; endoplasmic reticulum stress; inflammatory response |
| المشرفين على المادة: | 0 (Hspa5 protein, mouse) 0 (Endoplasmic Reticulum Chaperone BiP) 0 (Cystatins) 0 (Lipopolysaccharides) 0 (Tumor Necrosis Factor-alpha) 0 (Interleukin-6) 0 (Recombinant Proteins) 0 (Proto-Oncogene Proteins c-bcl-2) 0 (bcl-2-Associated X Protein) 0 (Antigens, Differentiation, Myelomonocytic) 0 (CD68 protein, mouse) 7535-00-4 (Galactosamine) 0 (Antigens, CD) 0 (CD68 Molecule) |
| تواريخ الأحداث: | Date Created: 20240708 Date Completed: 20240708 Latest Revision: 20240714 |
| رمز التحديث: | 20240714 |
| مُعرف محوري في PubMed: | PMC11237295 |
| DOI: | 10.12122/j.issn.1673-4254.2024.06.13 |
| PMID: | 38977342 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1673-4254 |
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| DOI: | 10.12122/j.issn.1673-4254.2024.06.13 |