دورية أكاديمية

Deciphering the interplay of gut microbiota and metabolomics in retinal vein occlusion.

التفاصيل البيبلوغرافية
العنوان: Deciphering the interplay of gut microbiota and metabolomics in retinal vein occlusion.
المؤلفون: Ai J; Department of Ophthalmology, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang Province, China., Cao Y; Hepatobiliary and Pancreatic Interventional Treatment Center, Division of Hepatobiliary and Pancreatic Surgery, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang Province, China.; Institute of Translational Medicine, Zhejiang University School of Medicine, Hangzhou, Zhejiang Province, China., Zhang C; Hepatobiliary and Pancreatic Interventional Treatment Center, Division of Hepatobiliary and Pancreatic Surgery, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang Province, China., Sun J-H; Hepatobiliary and Pancreatic Interventional Treatment Center, Division of Hepatobiliary and Pancreatic Surgery, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang Province, China., Dong F; Department of Ophthalmology, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang Province, China., Jing L; Hepatobiliary and Pancreatic Interventional Treatment Center, Division of Hepatobiliary and Pancreatic Surgery, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang Province, China., Wang J; Department of Ophthalmology, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang Province, China., Cui H; Department of Ophthalmology, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang Province, China.
المصدر: Microbiology spectrum [Microbiol Spectr] 2024 Aug 06; Vol. 12 (8), pp. e0005224. Date of Electronic Publication: 2024 Jul 09.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: ASM Press Country of Publication: United States NLM ID: 101634614 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2165-0497 (Electronic) Linking ISSN: 21650497 NLM ISO Abbreviation: Microbiol Spectr Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Washington, DC : ASM Press, 2013-
مواضيع طبية MeSH: Gastrointestinal Microbiome* , Retinal Vein Occlusion*/metabolism , Retinal Vein Occlusion*/microbiology , Feces*/microbiology , Bacteria*/classification , Bacteria*/metabolism , Bacteria*/genetics , Bacteria*/isolation & purification , Metabolomics* , RNA, Ribosomal, 16S*/genetics, Humans ; Male ; Middle Aged ; Female ; Aged ; Adult ; Chromatography, Liquid
مستخلص: This study aims to explore the link between retinal vein occlusion (RVO), a blinding ocular condition, and alterations in gut microbiota composition, to offer insights into the pathogenesis of RVO. Fecal samples from 25 RVO patients and 11 non-RVO individuals were analyzed using 16S rRNA sequencing and liquid chromatography-mass spectrometry (LC-MS). Significant differences in the abundance of gut microbial species were noted between RVO and non-RVO groups. At the phylum level, the RVO group showed an elevation in the ratio of Firmicutes to Bacteroidetes. At the genus level, the RVO group showed higher abundance in Escherichia_Shigella ( P < 0.05) and less abundance in Parabacteroides ( P < 0.01) than the non-RVO group. Functional predictions indicated reduced folate synthesis, biotin metabolism, and oxidative phosphorylation, with an increase in butyric acid metabolism in the RVO group. LC-MS analysis showed significant differences in purine metabolism, ABC transporters, and naphthalene degradation pathways, especially purine metabolism. Pearson correlation analysis revealed significant associations between bacterial genera and fecal metabolites. Enrichment analysis highlighted connections between specific metabolites and bacterial genera. The findings showed that the dysregulation of gut microbiota was observed in RVO patients, suggesting the gut microbiota as a potential therapeutic target. Modulating the gut microbiota could be a novel strategy for managing RVO and improving patient outcomes. Furthermore, the study findings suggest the involvement of gut microbial dysbiosis in RVO development, underscoring the significance of understanding its pathogenesis for effective treatment development.
Importance: Retinal vein occlusion (RVO) is a blinding ocular condition, and understanding its pathogenesis is crucial for developing effective treatments. This study demonstrates significant differences in gut microbiota composition between RVO patients and non-RVO individuals, implicating the involvement of gut microbial dysbiosis in RVO development. Functional predictions and metabolic profiling provide insights into the underlying mechanisms, highlighting potential pathways for therapeutic intervention. These findings suggest that modulating the gut microbiota might be a promising strategy for managing RVO and improving patient outcomes.
Competing Interests: The authors declare no conflict of interest.
معلومات مُعتمدة: 82102159 MOST | National Natural Science Foundation of China (NSFC); 81971713 MOST | National Natural Science Foundation of China (NSFC); 82102160 MOST | National Natural Science Foundation of China (NSFC)
فهرسة مساهمة: Keywords: 16S rRNA sequencing; gut microbiota; metabolomics; retinal vein occlusion
المشرفين على المادة: 0 (RNA, Ribosomal, 16S)
تواريخ الأحداث: Date Created: 20240709 Date Completed: 20240808 Latest Revision: 20240808
رمز التحديث: 20240808
مُعرف محوري في PubMed: PMC11302663
DOI: 10.1128/spectrum.00052-24
PMID: 38980030
قاعدة البيانات: MEDLINE
الوصف
تدمد:2165-0497
DOI:10.1128/spectrum.00052-24