دورية أكاديمية
أعرض في EDS

Expression and Functional Characterization of Oryzacystatin II Protein from Oryza sativa L. Indica Rice and Its Potential Role as a Bioinsecticide Against Major Insects of Rice.

التفاصيل البيبلوغرافية
العنوان: Expression and Functional Characterization of Oryzacystatin II Protein from Oryza sativa L. Indica Rice and Its Potential Role as a Bioinsecticide Against Major Insects of Rice.
المؤلفون: Premachandran K; Centre for Climate Change Studies, International Research Centre, Sathyabama Institute of Science and Technology, Chennai Tamil Nadu 600119, India., Shyu DJH; Department of Biological Science and Technology, National Pingtung University of Science and Technology, Neipu, Pingtung 912301, Taiwan., Vattikuti JL; Department of Entomology, ICAR-Indian Institute of Rice Research (ICAR-IIRR), Rajendranagar, Hyderabad 500030, India., Yanamadala S; Department of Biotechnology, Sathyabama Institute of Science and Technology, Chennai Tamil Nadu 600119, India., Srinivasan TS; Centre for Climate Change Studies, International Research Centre, Sathyabama Institute of Science and Technology, Chennai Tamil Nadu 600119, India.; IRRI South Asia Regional Center, Delhi 110012, India.
المصدر: Journal of agricultural and food chemistry [J Agric Food Chem] 2024 Jul 24; Vol. 72 (29), pp. 16378-16389. Date of Electronic Publication: 2024 Jul 09.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: American Chemical Society Country of Publication: United States NLM ID: 0374755 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1520-5118 (Electronic) Linking ISSN: 00218561 NLM ISO Abbreviation: J Agric Food Chem Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Washington, American Chemical Society.
مواضيع طبية MeSH: Oryza*/genetics , Oryza*/chemistry , Oryza*/metabolism , Insecticides*/chemistry , Insecticides*/pharmacology , Plant Proteins*/genetics , Plant Proteins*/chemistry , Plant Proteins*/metabolism , Molecular Docking Simulation*, Animals ; Pest Control, Biological
مستخلص: Rice ( Oryza sativa L.) is a crucial staple food crop globally, facing significant challenges from various pests that affect crop productivity and quality. Conventional pesticide usage has limitations, necessitating the development of sustainable pest management strategies. This study focuses on the expression, purification, and functional characterization of Oryzacystatin II (OC-II), a protein derived from O. sativa L. Indica rice, with the intent to evaluate its potential as a bioinsecticide against rice pests. The OC-II gene was expressed and purified, and purification confirmed its molecular weight (∼12 kDa) and protein sequence through LC-MS/MS analysis and Western blotting. The IC 50 value of OC-II was calculated as 0.06 μM, and the inhibition was identified as a competitive inhibition. The protein exhibited efficient control of both pests at the nymph and adult stages, with lower probing marks observed on treated plants. The inhibition of cathepsin B enzyme activity in insects further confirmed the bioactivity of the OC-II protein. Molecular docking and molecular dynamics simulations provided insights into the interaction between the OC-II protein and cathepsin enzymes reported in BPH and WBPH. Further investigations can focus on optimizing production methods and exploring the specificity and efficacy of the OC-II protein against other crop pests to enhance its practical applications.
فهرسة مساهمة: Keywords: Brown planthopper; Oryza sativa L. indica rice; bioinsecticide; oryzacystatin II; white-backed planthopper
المشرفين على المادة: 0 (Insecticides)
0 (Plant Proteins)
تواريخ الأحداث: Date Created: 20240709 Date Completed: 20240724 Latest Revision: 20240724
رمز التحديث: 20240725
DOI: 10.1021/acs.jafc.4c02055
PMID: 38980661
قاعدة البيانات: MEDLINE
الوصف
تدمد:1520-5118
DOI:10.1021/acs.jafc.4c02055