دورية أكاديمية
Enrichment culture evaluation and characterization of Streptococcus agalactiae among pregnant women in Japan.
| العنوان: | Enrichment culture evaluation and characterization of Streptococcus agalactiae among pregnant women in Japan. |
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| المؤلفون: | Nakano S; Antimicrobial Resistance Research Center, National Institute of Infectious Diseases, Tokyo, Japan., Koide S; Antimicrobial Resistance Research Center, National Institute of Infectious Diseases, Tokyo, Japan., Hosaka Y; Antimicrobial Resistance Research Center, National Institute of Infectious Diseases, Tokyo, Japan., Hasegawa Y; Department of Obstetrics and Gynecology, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan., Ishida-Kuroki K; Antimicrobial Resistance Research Center, National Institute of Infectious Diseases, Tokyo, Japan., Kawakami S; Antimicrobial Resistance Research Center, National Institute of Infectious Diseases, Tokyo, Japan., Hayashi W; Antimicrobial Resistance Research Center, National Institute of Infectious Diseases, Tokyo, Japan., Yu L; Antimicrobial Resistance Research Center, National Institute of Infectious Diseases, Tokyo, Japan., Kayama S; Antimicrobial Resistance Research Center, National Institute of Infectious Diseases, Tokyo, Japan., Miyashita N; Department of Obstetrics and Gynecology, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan., Nagata K; Department of Obstetrics and Gynecology, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan., Miura S; Department of Obstetrics and Gynecology, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan., Sugawara Y; Antimicrobial Resistance Research Center, National Institute of Infectious Diseases, Tokyo, Japan., Miyazaki H; Division of Infection Control and Prevention, Kokura Memorial Hospital, Kitakyushu, Japan., Miura K; Department of Obstetrics and Gynecology, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan., Sugai M; Antimicrobial Resistance Research Center, National Institute of Infectious Diseases, Tokyo, Japan. |
| المصدر: | Journal of medical microbiology [J Med Microbiol] 2024 Jul; Vol. 73 (7). |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Microbiology Society Country of Publication: England NLM ID: 0224131 Publication Model: Print Cited Medium: Internet ISSN: 1473-5644 (Electronic) Linking ISSN: 00222615 NLM ISO Abbreviation: J Med Microbiol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2015- : London : Microbiology Society Original Publication: 1968-1995: Edinburgh, Livingstone. |
| مواضيع طبية MeSH: | Streptococcus agalactiae*/genetics , Streptococcus agalactiae*/drug effects , Streptococcus agalactiae*/isolation & purification , Streptococcus agalactiae*/classification , Streptococcal Infections*/microbiology , Streptococcal Infections*/epidemiology , Anti-Bacterial Agents*/pharmacology , Vagina*/microbiology, Humans ; Female ; Pregnancy ; Japan/epidemiology ; Culture Media/chemistry ; Pregnancy Complications, Infectious/microbiology ; Pregnancy Complications, Infectious/epidemiology ; Rectum/microbiology ; Microbial Sensitivity Tests ; Whole Genome Sequencing ; Adult ; Clindamycin/pharmacology ; Genome, Bacterial |
| مستخلص: | Introduction. Maternal screening tests and prophylactic antibiotics are important to prevent neonatal and infant group B streptococcal (GBS) infections. Hypothesis/Gap Statement. The performance of enrichment broth media for GBS screening that are available in Japan is unclear. Whole-genome data of GBS isolates from pregnant women in Japan is lacking. Aim. The aim of this study was to compare the protocol performance of six enrichment broths and two subculture agar plates, which were all available in Japan, for GBS detection. In addition, we showed whole-genome data of GBS isolates from pregnant women in Japan. Methodology. We collected 133 vaginal-rectal swabs from pregnant women visiting clinics and hospitals in Nagasaki Prefecture, Japan, and compared the protocol performance of 6 enrichment broths and 2 subculture agar plates. All GBS isolates collected in this study were subjected to whole-genome sequencing analysis. Results. We obtained 133 vaginal-rectal swabs from pregnant women at 35-37 weeks of gestation from 8 private clinics and 2 local municipal hospitals within Nagasaki Prefecture, Japan. The detection rate of the protocol involving the six enrichment broths and subsequent subcultures varied between 95.5 and 100 %, depending on the specific choice of enrichment broth. The GBS carriage rate among pregnant women in this region was 18.8 %. All 25 isolates derived from the swabs were susceptible to penicillin, whereas 48 and 36 % of the isolates demonstrated resistance to erythromycin and clindamycin, respectively. The distribution of serotypes was highly diverse, encompassing seven distinct serotypes among the isolates, with the predominant serotype being serotype V ( n = 8). Serotype V isolates displayed a tendency towards increased resistance to erythromycin and clindamycin, with all resistant isolates containing the ermB gene. Conclusion. There was no difference in performance among the culture protocols evaluated in this study. GBS strains isolated from pregnant women appeared to have greater genomic diversity than GBS strains detected in neonates/infants with invasive GBS infections. To confirm this result, further studies with larger sample sizes are needed. |
| فهرسة مساهمة: | Keywords: GBS; Streptococcus agalactiae; performance; pregnant women; serotype; whole-genome sequencing |
| المشرفين على المادة: | 0 (Anti-Bacterial Agents) 0 (Culture Media) 3U02EL437C (Clindamycin) |
| تواريخ الأحداث: | Date Created: 20240710 Date Completed: 20240710 Latest Revision: 20240710 |
| رمز التحديث: | 20240710 |
| DOI: | 10.1099/jmm.0.001849 |
| PMID: | 38985141 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1473-5644 |
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| DOI: | 10.1099/jmm.0.001849 |