دورية أكاديمية
أعرض في EDS

Fungal melanin suppresses airway epithelial chemokine secretion through blockade of calcium fluxing.

التفاصيل البيبلوغرافية
العنوان: Fungal melanin suppresses airway epithelial chemokine secretion through blockade of calcium fluxing.
المؤلفون: Reedy JL; Department of Medicine, Division of Infectious Diseases, Massachusetts General Hospital, Boston, MA, USA.; Harvard Medical School, Boston, MA, USA., Jensen KN; Department of Medicine, Division of Infectious Diseases, Massachusetts General Hospital, Boston, MA, USA.; Harvard Medical School, Boston, MA, USA., Crossen AJ; Department of Medicine, Division of Infectious Diseases, Massachusetts General Hospital, Boston, MA, USA., Basham KJ; Department of Medicine, Division of Infectious Diseases, Massachusetts General Hospital, Boston, MA, USA., Ward RA; Department of Medicine, Division of Infectious Diseases, Massachusetts General Hospital, Boston, MA, USA., Reardon CM; Department of Medicine, Division of Infectious Diseases, Massachusetts General Hospital, Boston, MA, USA., Brown Harding H; Department of Medicine, Division of Infectious Diseases, Massachusetts General Hospital, Boston, MA, USA.; Harvard Medical School, Boston, MA, USA., Hepworth OW; Department of Medicine, Division of Infectious Diseases, Massachusetts General Hospital, Boston, MA, USA.; Harvard Medical School, Boston, MA, USA., Simaku P; Department of Medicine, Division of Infectious Diseases, Massachusetts General Hospital, Boston, MA, USA., Kwaku GN; Department of Medicine, Division of Infectious Diseases, Massachusetts General Hospital, Boston, MA, USA., Tone K; Aberdeen Fungal Group, University of Aberdeen, Institute of Medical Sciences, Foresterhill, Aberdeen, United Kingdom.; Division of Respiratory Diseases, Department of Internal Medicine, The Jikei University School of Medicine, Tokyo, Japan., Willment JA; Aberdeen Fungal Group, University of Aberdeen, Institute of Medical Sciences, Foresterhill, Aberdeen, United Kingdom.; MRC Centre for Medical Mycology, University of Exeter, Exeter, United Kingdom., Reid DM; Aberdeen Fungal Group, University of Aberdeen, Institute of Medical Sciences, Foresterhill, Aberdeen, United Kingdom., Stappers MHT; Aberdeen Fungal Group, University of Aberdeen, Institute of Medical Sciences, Foresterhill, Aberdeen, United Kingdom.; MRC Centre for Medical Mycology, University of Exeter, Exeter, United Kingdom., Brown GD; Aberdeen Fungal Group, University of Aberdeen, Institute of Medical Sciences, Foresterhill, Aberdeen, United Kingdom.; MRC Centre for Medical Mycology, University of Exeter, Exeter, United Kingdom., Rajagopal J; Center for Regenerative Medicine, Massachusetts General Hospital, Boston, MA, USA.; Division of Pulmonary and Critical Care Medicine, Department of Medicine, Massachusetts General Hospital, Boston, MA, USA.; Harvard Stem Cell Institute, Cambridge, MA, USA.; Klarman Cell Observatory, Broad Institute of Massachusetts Institute of Technology and Harvard, Cambridge, MA, USA., Vyas JM; Department of Medicine, Division of Infectious Diseases, Massachusetts General Hospital, Boston, MA, USA. jvyas@mgh.harvard.edu.; Harvard Medical School, Boston, MA, USA. jvyas@mgh.harvard.edu.
المصدر: Nature communications [Nat Commun] 2024 Jul 10; Vol. 15 (1), pp. 5817. Date of Electronic Publication: 2024 Jul 10.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Nature Pub. Group Country of Publication: England NLM ID: 101528555 Publication Model: Electronic Cited Medium: Internet ISSN: 2041-1723 (Electronic) Linking ISSN: 20411723 NLM ISO Abbreviation: Nat Commun Subsets: MEDLINE
أسماء مطبوعة: Original Publication: [London] : Nature Pub. Group
مواضيع طبية MeSH: Melanins*/metabolism , Aspergillus fumigatus* , Interleukin-8*/metabolism , Calcium*/metabolism , Chemokine CXCL1*/metabolism, Humans ; Animals ; Respiratory Mucosa/metabolism ; Respiratory Mucosa/microbiology ; Mice ; Epithelial Cells/metabolism ; Epithelial Cells/microbiology ; Chemokines/metabolism ; Mice, Inbred C57BL
مستخلص: Respiratory infections caused by the human fungal pathogen Aspergillus fumigatus are a major cause of mortality for immunocompromised patients. Exposure to these pathogens occurs through inhalation, although the role of the respiratory epithelium in disease pathogenesis has not been fully defined. Employing a primary human airway epithelial model, we demonstrate that fungal melanins potently block the post-translational secretion of the chemokines CXCL1 and CXCL8 independent of transcription or the requirement of melanin to be phagocytosed, leading to a significant reduction in neutrophil recruitment to the apical airway both in vitro and in vivo. Aspergillus-derived melanin, a major constituent of the fungal cell wall, dampened airway epithelial chemokine secretion in response to fungi, bacteria, and exogenous cytokines. Furthermore, melanin muted pathogen-mediated calcium fluxing and hindered actin filamentation. Taken together, our results reveal a critical role for melanin interaction with airway epithelium in shaping the host response to fungal and bacterial pathogens.
(© 2024. The Author(s).)
References: J Immunol. 1998 Oct 15;161(8):4332-9. (PMID: 9780210)
J Fungi (Basel). 2022 Dec 27;9(1):. (PMID: 36675861)
Infect Immun. 1995 Aug;63(8):3131-6. (PMID: 7622240)
N Engl J Med. 2021 Oct 14;385(16):1496-1509. (PMID: 34644473)
Autophagy. 2016 May 3;12(5):888-9. (PMID: 27028978)
J Vis Exp. 2014 Jan 06;(83):e50823. (PMID: 24430378)
Clin Respir J. 2019 Apr;13(4):202-211. (PMID: 30661296)
Cell Microbiol. 2019 May;21(5):e12994. (PMID: 30552790)
mBio. 2023 Feb 28;14(1):e0318422. (PMID: 36598192)
PLoS One. 2010 Aug 12;5(8):e12091. (PMID: 20711426)
Med Microbiol Immunol. 1998 Oct;187(2):79-89. (PMID: 9832321)
Trends Microbiol. 2001 Aug;9(8):382-9. (PMID: 11514221)
J Immunol. 2017 Oct 15;199(8):2873-2884. (PMID: 28887431)
Eur J Clin Microbiol Infect Dis. 2007 Dec;26(12):879-86. (PMID: 17874329)
Mol Microbiol. 1997 Oct;26(1):175-83. (PMID: 9383199)
Cell Host Microbe. 2016 Jan 13;19(1):79-90. (PMID: 26749442)
J Immunol. 2004 Nov 1;173(9):5712-20. (PMID: 15494523)
Annu Rev Microbiol. 2017 Sep 8;71:99-116. (PMID: 28701066)
Genetics. 2016 May;203(1):335-52. (PMID: 27017621)
Infect Chemother. 2014 Sep;46(3):209-15. (PMID: 25298912)
Arch Pathol Lab Med. 2008 Apr;132(4):606-14. (PMID: 18384212)
Am J Respir Crit Care Med. 2020 Sep 1;202(5):708-716. (PMID: 32407157)
Immunobiology. 2010 Nov;215(11):915-20. (PMID: 19939494)
Nat Microbiol. 2018 Jul;3(7):791-803. (PMID: 29849062)
Proc Natl Acad Sci U S A. 2004 May 11;101(19):7421-6. (PMID: 15123795)
J Bacteriol. 1998 Jun;180(12):3031-8. (PMID: 9620950)
Cell Mol Immunol. 2023 Mar;20(3):217-251. (PMID: 36725964)
Infect Immun. 1982 Jun;36(3):1175-84. (PMID: 6807845)
Exp Cell Res. 2006 May 15;312(9):1496-506. (PMID: 16499908)
mSphere. 2021 Oct 27;6(5):e0081921. (PMID: 34643424)
J Leukoc Biol. 2020 Feb;107(2):285-297. (PMID: 31841231)
J Biol Chem. 1957 May;226(1):497-509. (PMID: 13428781)
J Cyst Fibros. 2021 Nov;20(6):1062-1071. (PMID: 33589340)
Curr Opin Microbiol. 2020 Dec;58:160-165. (PMID: 33217703)
Curr Top Microbiol Immunol. 2020;425:17-28. (PMID: 32385534)
Cell Stem Cell. 2016 Aug 4;19(2):217-231. (PMID: 27320041)
J Clin Microbiol. 1983 Dec;18(6):1419-21. (PMID: 6418759)
Infect Immun. 2000 May;68(5):2845-53. (PMID: 10768981)
J Antimicrob Chemother. 2010 Oct;65(10):2116-8. (PMID: 20729241)
Am J Respir Cell Mol Biol. 2005 Aug;33(2):138-44. (PMID: 15879161)
Front Microbiol. 2011 May 03;2:96. (PMID: 21747802)
Clin Infect Dis. 2001 Feb 1;32(3):358-66. (PMID: 11170942)
Nat Commun. 2020 May 8;11(1):2282. (PMID: 32385235)
Blood Adv. 2017 Mar 22;1(9):557-568. (PMID: 29296975)
Int J Med Microbiol. 2014 Jul;304(5-6):626-36. (PMID: 24836942)
Int J Biol Macromol. 2021 Dec 1;192:745-756. (PMID: 34634335)
Mol Immunol. 2021 Apr;132:142-149. (PMID: 33588245)
Infect Immun. 1996 Jul;64(7):2420-4. (PMID: 8698461)
Clin Infect Dis. 2022 Jan 29;74(2):309-318. (PMID: 33876235)
Curr Top Med Mycol. 1988;2:338-87. (PMID: 3288360)
mBio. 2023 Jun 27;14(3):e0019423. (PMID: 37067432)
Med Mycol. 2017 Jan 1;55(1):39-47. (PMID: 27838644)
Bio Protoc. 2018 Jun 5;8(11):. (PMID: 30009215)
Cell Rep. 2021 Apr 20;35(3):109011. (PMID: 33882306)
Infect Immun. 1997 Dec;65(12):5110-7. (PMID: 9393803)
Clin Infect Dis. 2020 Dec 3;71(9):2459-2468. (PMID: 32358954)
Int J Biochem Cell Biol. 1997 Nov;29(11):1235-9. (PMID: 9451820)
PLoS One. 2010 Jul 26;5(7):e11754. (PMID: 20668677)
Microbiology (Reading). 2007 Dec;153(Pt 12):3954-3962. (PMID: 18048910)
Am J Respir Cell Mol Biol. 2019 Sep;61(3):322-331. (PMID: 30848657)
J Immunol. 2012 Nov 15;189(10):4960-9. (PMID: 23045615)
Nature. 2018 Mar 15;555(7696):382-386. (PMID: 29489751)
Am J Respir Cell Mol Biol. 2014 Feb;50(2):292-300. (PMID: 24010916)
J Cell Biol. 2004 Jul 5;166(1):11-5. (PMID: 15240566)
Sci Rep. 2017 Aug 15;7(1):8182. (PMID: 28811631)
Arch Microbiol. 2006 Nov;186(5):345-55. (PMID: 16988817)
Eur J Immunol. 2000 Feb;30(2):697-704. (PMID: 10671229)
Biochemistry. 2005 Mar 15;44(10):3683-93. (PMID: 15751945)
Prostaglandins Other Lipid Mediat. 2014 Jan;108:1-8. (PMID: 24315875)
J Exp Med. 1996 Aug 1;184(2):377-86. (PMID: 8760791)
Microbiology (Reading). 2017 Nov;163(11):1540-1556. (PMID: 29043954)
Integr Biol (Camb). 2012 Feb;4(2):220-7. (PMID: 22200052)
Nature. 1958 Nov 22;182(4647):1427-8. (PMID: 13600352)
Arch Bronconeumol. 2022 Jun;58(6):463-465. (PMID: 34866748)
PLoS Pathog. 2017 Aug 3;13(8):e1006548. (PMID: 28771621)
J Immunol. 2015 Feb 1;194(3):1304-15. (PMID: 25548217)
Nature. 2018 Aug;560(7718):319-324. (PMID: 30069044)
Infect Immun. 2020 Jan 22;88(2):. (PMID: 31767773)
معلومات مُعتمدة: 217163 United Kingdom WT_ Wellcome Trust; 102705 United Kingdom WT_ Wellcome Trust; R01 AI136529 United States AI NIAID NIH HHS; R01 AI150181 United States AI NIAID NIH HHS; R21 AI152499 United States AI NIAID NIH HHS; R01AI150181 U.S. Department of Health & Human Services | NIH | Center for Information Technology (Center for Information Technology, National Institutes of Health); United Kingdom WT_ Wellcome Trust; R01AI136529 U.S. Department of Health & Human Services | National Institutes of Health (NIH); K08AI14755 U.S. Department of Health & Human Services | National Institutes of Health (NIH)
المشرفين على المادة: 0 (Melanins)
0 (Interleukin-8)
SY7Q814VUP (Calcium)
0 (Chemokine CXCL1)
0 (CXCL8 protein, human)
0 (CXCL1 protein, human)
0 (Chemokines)
تواريخ الأحداث: Date Created: 20240710 Date Completed: 20240710 Latest Revision: 20240714
رمز التحديث: 20240714
مُعرف محوري في PubMed: PMC11237042
DOI: 10.1038/s41467-024-50100-x
PMID: 38987270
قاعدة البيانات: MEDLINE
الوصف
تدمد:2041-1723
DOI:10.1038/s41467-024-50100-x