Integrated translation and metabolism in a partially self-synthesizing biochemical network.

التفاصيل البيبلوغرافية
العنوان:	Integrated translation and metabolism in a partially self-synthesizing biochemical network.
المؤلفون:	Giaveri S; Department of Biochemistry and Synthetic Metabolism, Max Planck Institute for Terrestrial Microbiology, Marburg, Germany., Bohra N; Department of Biochemistry and Synthetic Metabolism, Max Planck Institute for Terrestrial Microbiology, Marburg, Germany.; Max Planck School Matter to Life, Heidelberg, Germany., Diehl C; Department of Biochemistry and Synthetic Metabolism, Max Planck Institute for Terrestrial Microbiology, Marburg, Germany., Yang HY; Department of Biochemistry and Synthetic Metabolism, Max Planck Institute for Terrestrial Microbiology, Marburg, Germany.; Max Planck School Matter to Life, Heidelberg, Germany., Ballinger M; Department of Biochemistry and Synthetic Metabolism, Max Planck Institute for Terrestrial Microbiology, Marburg, Germany., Paczia N; Core Facility for Metabolomics and Small Molecule Mass Spectrometry, Max Planck Institute for Terrestrial Microbiology, Marburg, Germany., Glatter T; Facility for Mass Spectrometry and Proteomics, Max Planck Institute for Terrestrial Microbiology, Marburg, Germany., Erb TJ; Department of Biochemistry and Synthetic Metabolism, Max Planck Institute for Terrestrial Microbiology, Marburg, Germany.; SYNMIKRO Center for Synthetic Microbiology, Marburg, Germany.
المصدر:	Science (New York, N.Y.) [Science] 2024 Jul 12; Vol. 385 (6705), pp. 174-178. Date of Electronic Publication: 2024 Jul 11.
نوع المنشور:	Journal Article
اللغة:	English
بيانات الدورية:	Publisher: American Association for the Advancement of Science Country of Publication: United States NLM ID: 0404511 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1095-9203 (Electronic) Linking ISSN: 00368075 NLM ISO Abbreviation: Science Subsets: MEDLINE
أسماء مطبوعة:	Publication: : Washington, DC : American Association for the Advancement of Science Original Publication: New York, N.Y. : [s.n.] 1880-
مواضيع طبية MeSH:	Carbon Dioxide/metabolism , Cell-Free System , Glycine/biosynthesis , Glycine/genetics , Metabolic Networks and Pathways* , Protein Biosynthesis*, Acyl Coenzyme A/metabolism ; Feedback, Physiological ; Gene Regulatory Networks
مستخلص:	One of the hallmarks of living organisms is their capacity for self-organization and regeneration, which requires a tight integration of metabolic and genetic networks. We sought to construct a linked metabolic and genetic network in vitro that shows such lifelike behavior outside of a cellular context and generates its own building blocks from nonliving matter. We integrated the metabolism of the crotonyl-CoA/ethyl-malonyl-CoA/hydroxybutyryl-CoA cycle with cell-free protein synthesis using recombinant elements. Our network produces the amino acid glycine from CO 2 and incorporates it into target proteins following DNA-encoded instructions. By orchestrating ~50 enzymes we established a basic cell-free operating system in which genetically encoded inputs into a metabolic network are programmed to activate feedback loops allowing for self-integration and (partial) self-regeneration of the complete system.
المشرفين على المادة:	0 (Acyl Coenzyme A) 142M471B3J (Carbon Dioxide) TE7660XO1C (Glycine)
تواريخ الأحداث:	Date Created: 20240711 Date Completed: 20240711 Latest Revision: 20240712
رمز التحديث:	20240713
DOI:	10.1126/science.adn3856
PMID:	38991083
قاعدة البيانات:	MEDLINE

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تدمد:	1095-9203
DOI:	10.1126/science.adn3856