دورية أكاديمية
CISD2 downregulation participates in the ferroptosis process of human ovarian SKOV-3 cells through modulating the wild type p53-mediated GLS2/SAT1/SLC7A11 and Gpx4/TRF signaling pathway.
| العنوان: | CISD2 downregulation participates in the ferroptosis process of human ovarian SKOV-3 cells through modulating the wild type p53-mediated GLS2/SAT1/SLC7A11 and Gpx4/TRF signaling pathway. |
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| المؤلفون: | Wang Y; School of Basic Medicine, Chongqing Medical University, Chongqing, China; School of Basic Medicine, North Sichuan Medical College, Nanchong, China., Yi Y; School of Basic Medicine, Chongqing Medical University, Chongqing, China. Electronic address: silver_19@163.com. |
| المصدر: | Tissue & cell [Tissue Cell] 2024 Aug; Vol. 89, pp. 102458. Date of Electronic Publication: 2024 Jul 02. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Churchill Livingstone Country of Publication: Scotland NLM ID: 0214745 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1532-3072 (Electronic) Linking ISSN: 00408166 NLM ISO Abbreviation: Tissue Cell Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Edinburgh : Churchill Livingstone Original Publication: Edinburgh, Oliver & Boyd. |
| مواضيع طبية MeSH: | Ferroptosis*/genetics , Tumor Suppressor Protein p53*/metabolism , Tumor Suppressor Protein p53*/genetics , Down-Regulation*/genetics , Ovarian Neoplasms*/pathology , Ovarian Neoplasms*/metabolism , Ovarian Neoplasms*/genetics , Signal Transduction* , Phospholipid Hydroperoxide Glutathione Peroxidase*/metabolism , Phospholipid Hydroperoxide Glutathione Peroxidase*/genetics, Humans ; Female ; Cell Line, Tumor ; Cell Movement/genetics ; Gene Expression Regulation, Neoplastic ; Amino Acid Transport System y+/metabolism ; Amino Acid Transport System y+/genetics |
| مستخلص: | CISD2 and ferroptosis participate in cancer development, but are rarely reported in ovarian cancer. This study aimed to clarify interaction between CISD2 and ferroptosis and evaluate related mechanisms. si-CISD2, wt-p53 and mut-p53 lentiviruses were transfected into SKOV-3 cells. CISD2 and p53 (wild/mutant p53) gene transcriptions were evaluated by RT-PCR. Cell viability, invasion ability, and migration capacity were determined. Expressions of ferroptosis-associated CISD2, p53, elastin, β-catenin and levels of Gpx4 and TRF were examined. CISD2 downregulation (si-CISD2) has a significant inhibitory effect on cell activity and exerts a synergistic effect with p53. si-CISD2 and Wt-p53 markedly inhibited SKOV-3 invasion and migration capacity, compared to the downregulation control (si-NC) and overexpression control (ov-NC) group (p < 0.001). p53 expression was increased significantly in si-CISD2 treated SKOV-3, compared to si-NC treated cells (p < 0.05). si-CISD2 markedly decreased elastin and β-catenin expression compared to the si-NC and ov-NC group (p < 0.001). si-CISD2 modulated ferroptosis-associated molecules (CDKN1A, GLS2, SAT1, SLC7A11), decreased Gpx4 and increased TRF levels in SKOV-3. si-CISD2 and Wt-p53 played an obvious synergistic role in regulating ferroptosis-associated molecules and Gpx4/TRF pathway molecules. In conclusion, CISD2 downregulation was involved in ferroptosis process of SKOV-3 cells. This effect of CISD2 was mediated by wild-type p53-associated GLS2/SAT1/SLC7A11 and Gpx4/TRF pathway. Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2024 Elsevier Ltd. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Apoptosis; CISD2; Ferroptosis; Gpx4; p53 |
| المشرفين على المادة: | 0 (Tumor Suppressor Protein p53) EC 1.11.1.12 (Phospholipid Hydroperoxide Glutathione Peroxidase) 0 (Amino Acid Transport System y+) 0 (SLC7A11 protein, human) 0 (TP53 protein, human) |
| تواريخ الأحداث: | Date Created: 20240711 Date Completed: 20240728 Latest Revision: 20240731 |
| رمز التحديث: | 20240731 |
| DOI: | 10.1016/j.tice.2024.102458 |
| PMID: | 38991271 |
| قاعدة البيانات: | MEDLINE |
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Full Text Finder | تدمد: | 1532-3072 |
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| DOI: | 10.1016/j.tice.2024.102458 |