دورية أكاديمية
Unveiling the role of circRBBP7 in myoblast proliferation and differentiation: A novel regulator of muscle development.
العنوان: | Unveiling the role of circRBBP7 in myoblast proliferation and differentiation: A novel regulator of muscle development. |
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المؤلفون: | Yang Y; State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, College of Animal Science and Technology, Guangxi University, Nanning, China.; The Animal Husbandry Research Institute of Guangxi Zhuang Autonomous Region, Guangxi Agricultural Vocational University, Nanning, China., Huang K; State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, College of Animal Science and Technology, Guangxi University, Nanning, China.; Guangdong Provincial Key Laboratory of Animal Molecular Design and Precise Breeding, School of Life Science and Engineering, Foshan University, Foshan, China., Jiang H; State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, College of Animal Science and Technology, Guangxi University, Nanning, China., Wang S; State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, College of Animal Science and Technology, Guangxi University, Nanning, China., Xu X; State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, College of Animal Science and Technology, Guangxi University, Nanning, China., Liu Y; Guangxi Zhuang Autonomous Region Center for Analysis and Test Research, Nanning, China., Liu Q; Guangdong Provincial Key Laboratory of Animal Molecular Design and Precise Breeding, School of Life Science and Engineering, Foshan University, Foshan, China., Wei M; The Animal Husbandry Research Institute of Guangxi Zhuang Autonomous Region, Guangxi Agricultural Vocational University, Nanning, China., Li Z; State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, College of Animal Science and Technology, Guangxi University, Nanning, China. |
المصدر: | FASEB journal : official publication of the Federation of American Societies for Experimental Biology [FASEB J] 2024 Jul 31; Vol. 38 (14), pp. e23808. |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: Federation of American Societies for Experimental Biology Country of Publication: United States NLM ID: 8804484 Publication Model: Print Cited Medium: Internet ISSN: 1530-6860 (Electronic) Linking ISSN: 08926638 NLM ISO Abbreviation: FASEB J Subsets: MEDLINE |
أسماء مطبوعة: | Publication: 2020- : [Bethesda, Md.] : Hoboken, NJ : Federation of American Societies for Experimental Biology ; Wiley Original Publication: [Bethesda, Md.] : The Federation, [c1987- |
مواضيع طبية MeSH: | Cell Differentiation* , Cell Proliferation* , Muscle Development*/physiology , Myoblasts*/metabolism , Myoblasts*/cytology , RNA, Circular*/genetics , RNA, Circular*/metabolism, Animals ; Male ; Mice ; Cell Line ; Mice, Inbred C57BL ; Muscle, Skeletal/metabolism ; Muscle, Skeletal/cytology ; Proto-Oncogene Proteins c-mdm2/metabolism ; Proto-Oncogene Proteins c-mdm2/genetics ; Signal Transduction ; Tumor Suppressor Protein p53/metabolism ; Tumor Suppressor Protein p53/genetics |
مستخلص: | Muscle development is a multistep process regulated by diverse gene networks, and circRNAs are considered novel regulators mediating myogenesis. Here, we systematically analyzed the role and underlying regulatory mechanisms of circRBBP7 in myoblast proliferation and differentiation. Results showed that circRBBP7 has a typical circular structure and encodes a 13 -kDa protein. By performing circRBBP7 overexpression and RNA interference, we found that the function of circRBBP7 was positively correlated with the proliferation and differentiation of myoblasts. Using RNA sequencing, we identified 1633 and 532 differentially expressed genes (DEGs) during myoblast proliferation or differentiation, respectively. The DEGs were found mainly enriched in cell cycle- and skeletal muscle development-related pathways, such as the MDM2/p53 and PI3K-Akt signaling pathways. Further co-IP and IF co-localization analysis revealed that VEGFR-1 is a target of circRBBP7 in myoblasts. qRT-PCR and WB analysis further confirmed the positive correlation between VEGFR-1 and circRBBP7. Moreover, we found that in vivo transfection of circRBBP7 into injured muscle tissues significantly promoted the regeneration and repair of myofibers in mice. Therefore, we speculate that circRBBP7 may affect the activity of MDM2 by targeting VEGFR-1, altering the expression of muscle development-related genes by mediating p53 degradation, and ultimately promoting myoblast development and muscle regeneration. This study provides essential evidence that circRBBP7 can serve as a potential target for myogenesis regulation and a reference for the application of circRBBP7 in cattle genetic breeding and muscle injury treatment. (© 2024 Federation of American Societies for Experimental Biology.) |
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معلومات مُعتمدة: | U20A2051 MOST | National Natural Science Foundation of China (NSFC); 2023GXNSFAA026350 Natural Science Foundation of Guangxi Zhuang Autonomous Region (Guangxi Natural Science Foundation); 20210006 Bama County for Talents in Science and Technology; 20210007 Bama County for Talents in Science and Technology; XKJ2333 Natural Science Foundation of Guangxi Agricultural Vocational University |
فهرسة مساهمة: | Keywords: VEGFR‐1; circRBBP7; muscle repair; myogenesis; proliferation and differentiation |
المشرفين على المادة: | EC 2.3.2.27 (Mdm2 protein, mouse) EC 2.3.2.27 (Proto-Oncogene Proteins c-mdm2) 0 (RNA, Circular) 0 (Tumor Suppressor Protein p53) 0 (Rbbp7 protein, mouse) |
تواريخ الأحداث: | Date Created: 20240712 Date Completed: 20240712 Latest Revision: 20240718 |
رمز التحديث: | 20240718 |
DOI: | 10.1096/fj.202302599RR |
PMID: | 38994637 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1530-6860 |
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DOI: | 10.1096/fj.202302599RR |