دورية أكاديمية
Molecular and cellular mechanisms of teneurin signaling in synaptic partner matching.
| العنوان: | Molecular and cellular mechanisms of teneurin signaling in synaptic partner matching. |
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| المؤلفون: | Xu C; Department of Biology and Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA; Biology Graduate Program, Stanford University, Stanford, CA 94305, USA., Li Z; Department of Biology and Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA; Biology Graduate Program, Stanford University, Stanford, CA 94305, USA., Lyu C; Department of Biology and Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA., Hu Y; Department of Biology and Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA; Biology Graduate Program, Stanford University, Stanford, CA 94305, USA., McLaughlin CN; Department of Biology and Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA., Wong KKL; Department of Biology and Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA., Xie Q; Department of Biology and Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA; Neurosciences Graduate Program, Stanford University, Stanford, CA 94305, USA., Luginbuhl DJ; Department of Biology and Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA., Li H; Department of Biology and Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA., Udeshi ND; The Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA., Svinkina T; The Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA., Mani DR; The Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA., Han S; Departments of Genetics, Biology, and Chemistry, Chan Zuckerberg Biohub, Stanford University, Stanford, CA 94305, USA., Li T; Department of Biology and Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA., Li Y; Department of Biology and Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA; Biology Graduate Program, Stanford University, Stanford, CA 94305, USA., Guajardo R; Department of Biology and Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA., Ting AY; Departments of Genetics, Biology, and Chemistry, Chan Zuckerberg Biohub, Stanford University, Stanford, CA 94305, USA., Carr SA; The Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA., Li J; Department of Biology and Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA; Biology Graduate Program, Stanford University, Stanford, CA 94305, USA; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA. Electronic address: lij6@janelia.hhmi.org., Luo L; Department of Biology and Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA. Electronic address: lluo@stanford.edu. |
| المصدر: | Cell [Cell] 2024 Jul 03. Date of Electronic Publication: 2024 Jul 03. |
| Publication Model: | Ahead of Print |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Cell Press Country of Publication: United States NLM ID: 0413066 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1097-4172 (Electronic) Linking ISSN: 00928674 NLM ISO Abbreviation: Cell Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Cambridge, Ma : Cell Press Original Publication: Cambridge, MIT Press. |
| مستخلص: | In developing brains, axons exhibit remarkable precision in selecting synaptic partners among many non-partner cells. Evolutionarily conserved teneurins are transmembrane proteins that instruct synaptic partner matching. However, how intracellular signaling pathways execute teneurins' functions is unclear. Here, we use in situ proximity labeling to obtain the intracellular interactome of a teneurin (Ten-m) in the Drosophila brain. Genetic interaction studies using quantitative partner matching assays in both olfactory receptor neurons (ORNs) and projection neurons (PNs) reveal a common pathway: Ten-m binds to and negatively regulates a RhoGAP, thus activating the Rac1 small GTPases to promote synaptic partner matching. Developmental analyses with single-axon resolution identify the cellular mechanism of synaptic partner matching: Ten-m signaling promotes local F-actin levels and stabilizes ORN axon branches that contact partner PN dendrites. Combining spatial proteomics and high-resolution phenotypic analyses, this study advanced our understanding of both cellular and molecular mechanisms of synaptic partner matching. Competing Interests: Declaration of interests L.L. is a member of the advisory board for Cell. (Copyright © 2024 The Author(s). Published by Elsevier Inc. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Drosophila; Rho GTPases; RhoGAP; branch stabilization; homophilic attraction; olfactory circuit; olfactory receptor neurons; projection neurons; synaptic partner matching; teneurin |
| تواريخ الأحداث: | Date Created: 20240712 Latest Revision: 20240712 |
| رمز التحديث: | 20240713 |
| DOI: | 10.1016/j.cell.2024.06.022 |
| PMID: | 38996528 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1097-4172 |
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| DOI: | 10.1016/j.cell.2024.06.022 |