دورية أكاديمية
Characterization of predominant genotype (QX) of avian infectious bronchitis virus isolated from layer chickens in Bangladesh.
| العنوان: | Characterization of predominant genotype (QX) of avian infectious bronchitis virus isolated from layer chickens in Bangladesh. |
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| المؤلفون: | Parvin MK; Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh., Sadekuzzaman M; Department of Livestock Services, Central Disease Investigation Laboratory, Dhaka 1000, Bangladesh., Haque ME; Department of Biochemistry and Molecular Biology, Sheikh Hasina University of Science and Technology, Bhairab 2350, Bangladesh., Haque MA; Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh., Kamal MM; Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh., Hayat S; Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh., Rahman MT; Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh., Siddique MP; Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh., Nahar SS; Livestock Research Institute, Mohakhali, Dhaka 1213, Bangladesh., Khasruzzaman AKM; Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh., Hossain MT; Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh., Islam MA; Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh. Electronic address: alimul.vmh@bau.edu.bd. |
| المصدر: | Poultry science [Poult Sci] 2024 Sep; Vol. 103 (9), pp. 103992. Date of Electronic Publication: 2024 Jun 24. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: England NLM ID: 0401150 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1525-3171 (Electronic) Linking ISSN: 00325791 NLM ISO Abbreviation: Poult Sci Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2020- : [Cambridge, UK] : Elsevier Original Publication: Champaign Il : Poultry Science Association |
| مواضيع طبية MeSH: | Infectious bronchitis virus*/genetics , Infectious bronchitis virus*/isolation & purification , Chickens* , Poultry Diseases*/virology , Poultry Diseases*/epidemiology , Coronavirus Infections*/veterinary , Coronavirus Infections*/virology , Coronavirus Infections*/epidemiology , Genotype*, Animals ; Bangladesh/epidemiology ; Phylogeny ; Female |
| مستخلص: | Avian infectious bronchitis (AIB) is a highly transmissible infection that affects the poultry industry globally. This study aims to isolate and characterize emerging strains of infectious bronchitis virus (IBV) from field samples of layer chickens in Bangladesh. A total of 108 samples (trachea, lung, and kidney) were taken from dead and sick layer chickens from 18 farms in 4 areas detecting outbreaks in Bangladesh. The samples were processed and inoculated in embryonated chicken eggs (ECEs) and finally screened by the trypsin-induced hemagglutination (THA) test. Using various techniques such as hemagglutination inhibition (HI), agar gel immuno-diffusion (AGID), virus neutralization test (VNT), reverse transcription-polymerase chain reaction (RT-PCR), and nucleotide sequencing, we were able to identify and confirm the isolated IBV viruses. The study also determined the hemagglutination (HA) pattern of isolated virus using avian and mammalian red blood cells. The pathogenicity of the isolated IBV was determined using embryonated chicken eggs and day-old chicks. The study found that 8 samples were positive for IBV using ECEs, and 4 were positive by the THA test. These isolates were confirmed using HI, AGID, and VN tests. S1 gene-based RT-PCR confirmed all four isolates as IBV, with the recent isolates belonging to the genotype-QX and being similar to IBV isolates from Thailand, Saudi Arabia, and India. The HA pattern of the recent isolates showed that the isolated IBV was virulent. The pathogenicity test also revealed that the four isolates were highly pathogenic. The study indicated that the prevalent genotype (QX) of the IBV strain is present in the layer chicken population of Bangladesh. Competing Interests: DISCLOSURES The authors declare no conflicts of interest. (Copyright © 2024. Published by Elsevier Inc.) |
| فهرسة مساهمة: | Keywords: ECEs; IBV; RT-PCR; nucleotide sequencing; pathogenicity |
| تواريخ الأحداث: | Date Created: 20240712 Date Completed: 20240824 Latest Revision: 20240824 |
| رمز التحديث: | 20240826 |
| مُعرف محوري في PubMed: | PMC11283218 |
| DOI: | 10.1016/j.psj.2024.103992 |
| PMID: | 38996739 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1525-3171 |
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| DOI: | 10.1016/j.psj.2024.103992 |