Pipette-tip solid-phase extraction coupled with matrix-assisted laser desorption/ionization mass spectrometry enables rapid and high-throughput analysis of antidepressants in rat serum.

التفاصيل البيبلوغرافية
العنوان:	Pipette-tip solid-phase extraction coupled with matrix-assisted laser desorption/ionization mass spectrometry enables rapid and high-throughput analysis of antidepressants in rat serum.
المؤلفون:	Sun Z; Department of Pharmacy, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, China., Wang F; Department of Pharmacy, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, China., Li W; Henan Key Laboratory of Nanomedicine for Targeting Diagnosis and Treatment, School of Pharmaceutical Sciences, Zhengzhou University, Zhengzhou, 450001, China., Ren R; Department of Pharmacy, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, China., Zhou P; Department of Pharmacy, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, China., Jia Q; Department of Pharmacy, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, China., Zhao L; Shenzhen Bao'an District Center for Disease Control and Prevention, Shenzhen, 518101, China., Chen D; Henan Key Laboratory of Nanomedicine for Targeting Diagnosis and Treatment, School of Pharmaceutical Sciences, Zhengzhou University, Zhengzhou, 450001, China. dichen@zzu.edu.cn., Zuo L; Department of Pharmacy, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, China. zuolihua2013@126.com.
المصدر:	Analytical and bioanalytical chemistry [Anal Bioanal Chem] 2024 Sep; Vol. 416 (22), pp. 5013-5023. Date of Electronic Publication: 2024 Jul 13.
نوع المنشور:	Journal Article
اللغة:	English
بيانات الدورية:	Publisher: Springer-Verlag Country of Publication: Germany NLM ID: 101134327 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1618-2650 (Electronic) Linking ISSN: 16182642 NLM ISO Abbreviation: Anal Bioanal Chem Subsets: MEDLINE
أسماء مطبوعة:	Original Publication: Heidelberg : Springer-Verlag, 2002-
مواضيع طبية MeSH:	Solid Phase Extraction/methods , Antidepressive Agents/blood , Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization*/methods, Animals ; Rats ; Limit of Detection ; High-Throughput Screening Assays/methods ; Rats, Sprague-Dawley ; Drug Monitoring/methods ; Male
مستخلص:	Therapeutic drug monitoring is essential for ensuring the efficacy and safety of medications. This study introduces a streamlined approach that combines pipette-tip solid-phase extraction (PT-SPE) with matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS), facilitating rapid and high-throughput monitoring of drug concentrations. As a demonstration, this method was applied to the extraction and quantification of antidepressants in serum. Utilizing Zip-Tip C18, the method enabled the extraction of antidepressants from complex biological matrices in less than 2 min, with the subsequent MALDI-MS analysis yielding results in just 1 min. Optimal extraction recoveries were achieved using a sampling solution at pH 9.0 and a 10 μL ethanol desorption solution containing 0.1% phosphoric acid. For MALDI analysis, 2,5-dihydroxybenzoic acid was identified as the most effective matrix for producing the highest signal intensity. The quantification strategy exhibited robust linearities (R ² ≥ 0.997) and satisfactory limits of quantification, ranging from 0.05 to 0.5 μg/mL for a suite of antidepressants. The application for monitoring dynamic concentration changes of antidepressants in rat serum emphasized the method's efficacy. This strategy offers the advantages of high throughput, minimal sample usage, environmental sustainability, and simplicity, providing ideas and a reference basis for the subsequent development of methods for therapeutic drug monitoring. (© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH, DE part of Springer Nature.)
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معلومات مُعتمدة:	No.82003921 National Natural Science Foundation of China; No.82374018 National Natural Science Foundation of China; 2021YFC2401105 National Key R&D Program of China; 242102311184 Henan Provincial Science and Technology Research Project; 2021M702937 China Postdoctoral Science Foundation; 2023M733256 China Postdoctoral Science Foundation
فهرسة مساهمة:	Keywords: Antidepressants; Matrix-assisted laser desorption/ionization mass spectrometry; Pipette-tip solid-phase extraction; Therapeutic drug monitoring
المشرفين على المادة:	0 (Antidepressive Agents)
تواريخ الأحداث:	Date Created: 20240712 Date Completed: 20240817 Latest Revision: 20240817
رمز التحديث:	20240818
DOI:	10.1007/s00216-024-05439-x
PMID:	38997460
قاعدة البيانات:	MEDLINE

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تدمد:	1618-2650
DOI:	10.1007/s00216-024-05439-x