دورية أكاديمية
Jasmonate-insensitive mutant jar1b prevents petal elongation and flower opening coupling with parthenocarpic fruit development in Cucurbita pepo.
| العنوان: | Jasmonate-insensitive mutant jar1b prevents petal elongation and flower opening coupling with parthenocarpic fruit development in Cucurbita pepo. |
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| المؤلفون: | Gautam K; Department of Biology and Geology. Agri-food Campus of International Excellence (CeiA3) and Research Center CIAIMBITAL, University of Almería, 04120, Almería, Spain., Segura M; Department of Biology and Geology. Agri-food Campus of International Excellence (CeiA3) and Research Center CIAIMBITAL, University of Almería, 04120, Almería, Spain., Alonso S; Department of Biology and Geology. Agri-food Campus of International Excellence (CeiA3) and Research Center CIAIMBITAL, University of Almería, 04120, Almería, Spain., Pasadas R; Department of Biology and Geology. Agri-food Campus of International Excellence (CeiA3) and Research Center CIAIMBITAL, University of Almería, 04120, Almería, Spain., García-Mina JM; Universidad de Navarra, Facultad de Ciencias, Departamento de Biología Ambiental, Grupo Química y Biología Agrícola, Irunlarrea 1, 31008, Pamplona, Spain., Zamarreño AM; Universidad de Navarra, Facultad de Ciencias, Departamento de Biología Ambiental, Grupo Química y Biología Agrícola, Irunlarrea 1, 31008, Pamplona, Spain., Martínez C; Department of Biology and Geology. Agri-food Campus of International Excellence (CeiA3) and Research Center CIAIMBITAL, University of Almería, 04120, Almería, Spain. Electronic address: cmartinez@ual.es., Jamilena M; Department of Biology and Geology. Agri-food Campus of International Excellence (CeiA3) and Research Center CIAIMBITAL, University of Almería, 04120, Almería, Spain. Electronic address: mjamille@ual.es. |
| المصدر: | Plant physiology and biochemistry : PPB [Plant Physiol Biochem] 2024 Sep; Vol. 214, pp. 108923. Date of Electronic Publication: 2024 Jul 07. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Science Country of Publication: France NLM ID: 9882449 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-2690 (Electronic) Linking ISSN: 09819428 NLM ISO Abbreviation: Plant Physiol Biochem Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Amsterdam : Elsevier Science Original Publication: Paris : Gauthier-Villars, c1987- |
| مواضيع طبية MeSH: | Cyclopentanes*/pharmacology , Cyclopentanes*/metabolism , Oxylipins*/pharmacology , Oxylipins*/metabolism , Flowers*/genetics , Flowers*/growth & development , Flowers*/drug effects , Cucurbita*/genetics , Cucurbita*/growth & development , Fruit*/genetics , Fruit*/growth & development , Fruit*/metabolism , Plant Proteins*/genetics , Plant Proteins*/metabolism, Isoleucine/pharmacology ; Isoleucine/metabolism ; Isoleucine/analogs & derivatives ; Mutation ; Gene Expression Regulation, Plant/drug effects ; Acetates/pharmacology ; Plant Roots/growth & development ; Plant Roots/genetics ; Plant Roots/metabolism ; Plant Roots/drug effects ; Plant Growth Regulators/metabolism ; Plant Growth Regulators/pharmacology |
| مستخلص: | Jasmonates are growth regulators that play a key role in flower development, fruit ripening, root growth, and plant defence. The study explores the coordination of floral organ maturation to ensure proper flower opening for pollination and fertilization. A new mutant (jar1b) was discovered, lacking petal elongation and flower opening but showing normal pistil and stamen development, leading to parthenocarpic fruit development. The mutation also enhanced the elongation of roots while reducing the formation of root hairs. BSA sequencing showed that jar1b is a missense mutation in the gene CpJAR1B, which encodes the enzyme that catalyzes the conjugation between JA and the amino acid isoleucine. The loss of function mutation in CpJAR1B produced a deficiency in biologically active (+) -7-iso-jasmonoyl-L-isoleucine (JA-Ile), which was not complemented by the paralogous gene CpJAR1A or any other redundant gene. Exogenous application of methyl jasmonate (MeJA) demonstrated that jar1b is partially insensitive to JA in both flowers and roots. Further experimentation involving the combination of JA-Ile deficient and ethylene-deficient, and ET insensitive mutations in double mutants revealed that CpJAR1B mediated ET action in female petal maturation and flower opening, but JA and ET have independent additive effects as negative regulators of the set and development of squash fruits. CpJAR1B also regulated the aperture of male flowers in an ethylene-independent manner. The root phenotype of jar1b and effects of external MeJA treatments indicated that CpJAR1B has a dual role in root development, inhibiting the elongation of primary and secondary roots, but promoting the formation of root hairs. Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2024 The Authors. Published by Elsevier Masson SAS.. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Anthesis; BSA-sequencing; Flower opening; Jasmonate; Parthenocarpy; Petal elongation |
| المشرفين على المادة: | 0 (Cyclopentanes) 0 (Oxylipins) 6RI5N05OWW (jasmonic acid) 0 (Plant Proteins) 04Y7590D77 (Isoleucine) 900N171A0F (methyl jasmonate) 0 (Acetates) 0 (Plant Growth Regulators) 0 (jasmonoyl-isoleucine) |
| تواريخ الأحداث: | Date Created: 20240713 Date Completed: 20240804 Latest Revision: 20240806 |
| رمز التحديث: | 20240807 |
| DOI: | 10.1016/j.plaphy.2024.108923 |
| PMID: | 39002308 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1873-2690 |
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| DOI: | 10.1016/j.plaphy.2024.108923 |