دورية أكاديمية
أعرض في EDS

A high-accuracy measurement procedure for salbutamol, ractopamine, and clenbuterol in pork by isotope dilution-liquid chromatography/tandem mass spectrometry.

التفاصيل البيبلوغرافية
العنوان: A high-accuracy measurement procedure for salbutamol, ractopamine, and clenbuterol in pork by isotope dilution-liquid chromatography/tandem mass spectrometry.
المؤلفون: Zhou X; Division of Chemical Metrology and Analytical Science, National Institute of Metrology, Beijing 100029, China; Key Laboratory of Chemical Metrology and Applications on Nutrition and Health for State Market Regulation, Beijing, 100029, China. Electronic address: zhouxia@nim.ac.cn., Tian T; Division of Chemical Metrology and Analytical Science, National Institute of Metrology, Beijing 100029, China; Shanghai University of Engineering Science, Shanghai, 201620, China., Li X; Division of Chemical Metrology and Analytical Science, National Institute of Metrology, Beijing 100029, China; Key Laboratory of Chemical Metrology and Applications on Nutrition and Health for State Market Regulation, Beijing, 100029, China. Electronic address: lixq@nim.ac.cn., Liu S; Shanghai University of Engineering Science, Shanghai, 201620, China., Zhang Q; Division of Chemical Metrology and Analytical Science, National Institute of Metrology, Beijing 100029, China; Key Laboratory of Chemical Metrology and Applications on Nutrition and Health for State Market Regulation, Beijing, 100029, China. Electronic address: zhangqh@nim.ac.cn.
المصدر: Food chemistry [Food Chem] 2024 Nov 30; Vol. 459, pp. 140357. Date of Electronic Publication: 2024 Jul 05.
نوع المنشور: Journal Article; Evaluation Study
اللغة: English
بيانات الدورية: Publisher: Elsevier Applied Science Publishers Country of Publication: England NLM ID: 7702639 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-7072 (Electronic) Linking ISSN: 03088146 NLM ISO Abbreviation: Food Chem Subsets: MEDLINE
أسماء مطبوعة: Publication: Barking : Elsevier Applied Science Publishers
Original Publication: Barking, Eng., Applied Science Publishers.
مواضيع طبية MeSH: Tandem Mass Spectrometry*/methods , Phenethylamines*/analysis , Phenethylamines*/chemistry , Clenbuterol*/analysis , Albuterol*/analysis , Albuterol*/chemistry, Animals ; Swine ; Chromatography, Liquid/methods ; Deuterium/chemistry ; Deuterium/analysis ; Indicator Dilution Techniques ; Chromatography, High Pressure Liquid/methods ; Food Contamination/analysis
مستخلص: In-depth research into the precise evaluation of enzymatic digestion efficiency and the selection of a suitable deuterium-labelled internal standard remains a gap in the accurate determination of β 2 -agonists in animal-derived food by isotope dilution-liquid chromatography/tandem mass spectrometry (ID-LC-MS/MS). In this study, the enzymatic digestion conditions were optimized by monitoring the presence of β 2 -agonist conjugates in positive samples, which proved to be reliable for ensuring complete enzymatic digestion. Comparative analysis of deuterium-labelled internal standards for salbutamol (SAL), ractopamine (RAC), and clenbuterol (CLB) revealed that CLB-D 6 and SAL-D 9 were less effective in compensating for matrix effects due to hydrogen‑deuterium exchange during MS fragment formation. Consequently, SAL-D 3 , RAC-D 3 and CLB-D 9 were chosen for the implementation of ID-LC-MS/MS. The developed method demonstrates high accuracy and precision, with the average recoveries ranging from 93.8% to 107.3% with RSD <6.1%, which can provide higher-order measurement results for β 2 -agonists in pork.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier Ltd. All rights reserved.)
فهرسة مساهمة: Keywords: Deuterium labelled internal standard; Enzymatic digestion; Isotope dilution mass spectrometry; β(2)-agonist conjugates; β(2)-agonists
المشرفين على المادة: 0 (Phenethylamines)
XTZ6AXU7KN (Clenbuterol)
57370OZ3P1 (ractopamine)
QF8SVZ843E (Albuterol)
AR09D82C7G (Deuterium)
تواريخ الأحداث: Date Created: 20240714 Date Completed: 20240822 Latest Revision: 20240822
رمز التحديث: 20240822
DOI: 10.1016/j.foodchem.2024.140357
PMID: 39003851
قاعدة البيانات: MEDLINE
الوصف
تدمد:1873-7072
DOI:10.1016/j.foodchem.2024.140357