دورية أكاديمية
أعرض في EDS

Comprehensive elucidation of the differential physiological kale response to cytokinins under in vitro conditions.

التفاصيل البيبلوغرافية
العنوان: Comprehensive elucidation of the differential physiological kale response to cytokinins under in vitro conditions.
المؤلفون: Kamińska M; Department of Plant Biochemistry, Faculty of Biology, University of Warsaw, Miecznikowa 1, Warsaw, 02-096, Poland. mkaminska@biol.uw.edu.pl., Styczynska A; Department of Plant Biochemistry, Faculty of Biology, University of Warsaw, Miecznikowa 1, Warsaw, 02-096, Poland., Szakiel A; Department of Plant Biochemistry, Faculty of Biology, University of Warsaw, Miecznikowa 1, Warsaw, 02-096, Poland., Pączkowski C; Department of Plant Biochemistry, Faculty of Biology, University of Warsaw, Miecznikowa 1, Warsaw, 02-096, Poland., Kućko A; Department of Plant Physiology, Institute of Biology, Warsaw University of Life Sciences- SGGW (WULS-SGGW), Nowoursynowska 159, Warsaw, 02-776, Poland.
المصدر: BMC plant biology [BMC Plant Biol] 2024 Jul 15; Vol. 24 (1), pp. 674. Date of Electronic Publication: 2024 Jul 15.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: BioMed Central Country of Publication: England NLM ID: 100967807 Publication Model: Electronic Cited Medium: Internet ISSN: 1471-2229 (Electronic) Linking ISSN: 14712229 NLM ISO Abbreviation: BMC Plant Biol Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London : BioMed Central, [2001-
مواضيع طبية MeSH: Cytokinins*/metabolism , Plant Growth Regulators*/metabolism , Plant Growth Regulators*/pharmacology , Kinetin*/pharmacology , Antioxidants*/metabolism, Brassica/drug effects ; Brassica/metabolism ; Brassica/physiology ; Brassica/growth & development ; Benzyl Compounds/pharmacology ; Purines ; Photosynthesis/drug effects ; Plant Shoots/drug effects ; Plant Shoots/metabolism ; Isopentenyladenosine/analogs & derivatives ; Isopentenyladenosine/metabolism ; Reactive Oxygen Species/metabolism
مستخلص: Background: Kale, a versatile cruciferous crop, valued for its pro-health benefits, stress resistance, and potential applications in forage and cosmetics, holds promise for further enhancement of its bioactive compounds through in vitro cultivation methods. Micropropagation techniques use cytokinins (CKs) which are characterized by various proliferative efficiency. Despite the extensive knowledge regarding CKs, there remains a gap in understanding their role in the physiological mechanisms. That is why, here we investigated the effects of three CKs - kinetin (Kin), 6-benzylaminopurine (BAP), and 2-isopentenyladenine (2iP) - on kale physiology, antioxidant status, steroidal metabolism, and membrane integrity under in vitro cultivation.
Results: Our study revealed that while BAP and 2iP stimulated shoot proliferation, they concurrently diminished pigment levels and photosynthetic efficiency. Heightened metabolic activity in response to all CKs was reflected by increased respiratory rate. Despite the differential burst of ROS, the antioxidant properties of kale were associated with the upregulation of guaiacol peroxidase and the scavenging properties of ascorbate rather than glutathione. Notably, CKs fostered the synthesis of sterols, particularly sitosterol, pivotal for cell proliferation and structure of membranes which are strongly disrupted under the action of BAP and 2iP possibly via pathway related to phospholipase D and lipoxygenase which were upregulated. Intriguingly, both CKs treatment spurred the accumulation of sitostenone, known for its ROS scavenging and therapeutic potential. The differential effects of CKs on brassicasterol levels and brassinosteroid (BRs) receptor suggest potential interactions between CKs and BRs.
Conclusion: Based on the presented results we conclude that the effect evoked by BAP and 2iP in vitro can improve the industrial significance of kale because this treatment makes possible to control proliferation and/or biosynthesis routes of valuable beneficial compounds. Our work offers significant insights into the nuanced effects of CKs on kale physiology and metabolism, illuminating potential avenues for their application in plant biotechnology and medicinal research.
(© 2024. The Author(s).)
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معلومات مُعتمدة: #501-D114-01-1140100 Uniwersytet Warszawski
فهرسة مساهمة: Keywords: Antioxidants; Cytokinins; In vitro plant culture; Kale; Micropropagation; Sterols
المشرفين على المادة: 0 (Cytokinins)
0 (Plant Growth Regulators)
P39Y9652YJ (Kinetin)
KXG6A989PS (benzylaminopurine)
0 (Antioxidants)
0 (Benzyl Compounds)
0 (Purines)
7724-76-7 (Isopentenyladenosine)
0 (Reactive Oxygen Species)
تواريخ الأحداث: Date Created: 20240714 Date Completed: 20240714 Latest Revision: 20240717
رمز التحديث: 20240717
مُعرف محوري في PubMed: PMC11247843
DOI: 10.1186/s12870-024-05396-8
PMID: 39004738
قاعدة البيانات: MEDLINE
الوصف
تدمد:1471-2229
DOI:10.1186/s12870-024-05396-8