دورية أكاديمية
أعرض في EDS

Anti-Staphy Peptides Rationally Designed from Cry10Aa Bacterial Protein.

التفاصيل البيبلوغرافية
العنوان: Anti-Staphy Peptides Rationally Designed from Cry10Aa Bacterial Protein.
المؤلفون: Rios TB; S-Inova Biotech, Programa de Pós-Graduação em Biotecnologia Universidade Católica Dom Bosco, Av. Tamandaré, 6000-Jardim Seminario, Campo Grande, MS 79117-900, Brazil.; Centro de Análises Proteômicas e Bioquímicas, Programa de Pós-Graduação em Ciências Genômicas e Biotecnologia Universidade Católica de Brasília, St. de Grandes Áreas Norte 916-Asa Norte, Brasília, DF 70790-160, Brazil., Maximiano MR; S-Inova Biotech, Programa de Pós-Graduação em Biotecnologia Universidade Católica Dom Bosco, Av. Tamandaré, 6000-Jardim Seminario, Campo Grande, MS 79117-900, Brazil.; Centro de Análises Proteômicas e Bioquímicas, Programa de Pós-Graduação em Ciências Genômicas e Biotecnologia Universidade Católica de Brasília, St. de Grandes Áreas Norte 916-Asa Norte, Brasília, DF 70790-160, Brazil., Fernandes FC; Centro de Análises Proteômicas e Bioquímicas, Programa de Pós-Graduação em Ciências Genômicas e Biotecnologia Universidade Católica de Brasília, St. de Grandes Áreas Norte 916-Asa Norte, Brasília, DF 70790-160, Brazil., Amorim GC; Centro de Análises Proteômicas e Bioquímicas, Programa de Pós-Graduação em Ciências Genômicas e Biotecnologia Universidade Católica de Brasília, St. de Grandes Áreas Norte 916-Asa Norte, Brasília, DF 70790-160, Brazil.; Embrapa Recursos Genéticos e Biotecnologia, Parque Estação Biológica, PqEB, Av. W5 Norte-Asa Norte, Brasília, DF 70770-917, Brazil., Porto WF; Porto Reports, Brasília, DF 70770-917, Brazil., Buccini DF; S-Inova Biotech, Programa de Pós-Graduação em Biotecnologia Universidade Católica Dom Bosco, Av. Tamandaré, 6000-Jardim Seminario, Campo Grande, MS 79117-900, Brazil., Nieto Marín V; S-Inova Biotech, Programa de Pós-Graduação em Biotecnologia Universidade Católica Dom Bosco, Av. Tamandaré, 6000-Jardim Seminario, Campo Grande, MS 79117-900, Brazil., Feitosa GC; Centro de Análises Proteômicas e Bioquímicas, Programa de Pós-Graduação em Ciências Genômicas e Biotecnologia Universidade Católica de Brasília, St. de Grandes Áreas Norte 916-Asa Norte, Brasília, DF 70790-160, Brazil.; Pós-Graduação em Patologia Molecular, Universidade de Brasília, Campus Darcy Ribeiro, Brasília, DF 70910-900, Brazil., Freitas CDP; Laboratório de RMN, Instituto de Química, Universidade Federal de Goiás, Goiânia, GO 74690-900, Brazil., Barra JB; Laboratório de RMN, Instituto de Química, Universidade Federal de Goiás, Goiânia, GO 74690-900, Brazil., Alonso A; Instituto de Física, Universidade Federal de Goiás, Goiânia, GO 74690-900, Brazil., Grossi de Sá MF; Centro de Análises Proteômicas e Bioquímicas, Programa de Pós-Graduação em Ciências Genômicas e Biotecnologia Universidade Católica de Brasília, St. de Grandes Áreas Norte 916-Asa Norte, Brasília, DF 70790-160, Brazil.; Embrapa Recursos Genéticos e Biotecnologia, Parque Estação Biológica, PqEB, Av. W5 Norte-Asa Norte, Brasília, DF 70770-917, Brazil., Lião LM; Laboratório de RMN, Instituto de Química, Universidade Federal de Goiás, Goiânia, GO 74690-900, Brazil., Franco OL; S-Inova Biotech, Programa de Pós-Graduação em Biotecnologia Universidade Católica Dom Bosco, Av. Tamandaré, 6000-Jardim Seminario, Campo Grande, MS 79117-900, Brazil.; Centro de Análises Proteômicas e Bioquímicas, Programa de Pós-Graduação em Ciências Genômicas e Biotecnologia Universidade Católica de Brasília, St. de Grandes Áreas Norte 916-Asa Norte, Brasília, DF 70790-160, Brazil.
المصدر: ACS omega [ACS Omega] 2024 Jun 19; Vol. 9 (27), pp. 29159-29174. Date of Electronic Publication: 2024 Jun 19 (Print Publication: 2024).
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: American Chemical Society Country of Publication: United States NLM ID: 101691658 Publication Model: eCollection Cited Medium: Internet ISSN: 2470-1343 (Electronic) Linking ISSN: 24701343 NLM ISO Abbreviation: ACS Omega Subsets: PubMed not MEDLINE
أسماء مطبوعة: Original Publication: Washington, D.C. : American Chemical Society, [2016]-
مستخلص: Bacterial infections pose a significant threat to human health, constituting a major challenge for healthcare systems. Antibiotic resistance is particularly concerning in the context of treating staphylococcal infections. In addressing this challenge, antimicrobial peptides (AMPs), characterized by their hydrophobic and cationic properties, unique mechanism of action, and remarkable bactericidal and immunomodulatory capabilities, emerge as promising alternatives to conventional antibiotics for tackling bacterial multidrug resistance. This study focuses on the Cry10Aa protein as a template for generating AMPs due to its membrane-penetrating ability. Leveraging the Joker algorithm, six peptide variants were derived from α-helix 3 of Cry10Aa, known for its interaction with lipid bilayers. In vitro, antimicrobial assays determined the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) required for inhibiting the growth of Staphylococcus aureus , Escherichia coli , Acinetobacter baummanii , Enterobacter cloacae , Enterococcus facallis , Klebsiella pneumonia , and Pseudomonas aeruginosa . Time-kill kinetics were performed using the parental peptide AMPCry10Aa, as well as AMPCry10Aa_1 and AMPCry10Aa_5, against E. coli ATCC, S. aureus 111 and S. aureus ATCC strains showing that AMPCry10Aa_1 and AMPCry10Aa_5 peptides can completely reduce the initial bacterial load with less than 2 h of incubation. AMPCry10Aa_1 and AMPCry 10Aa_5 present stability in human serum and activity maintenance up to 37 °C. Cytotoxicity assays, conducted using the MTT method, revealed that all of the tested peptides exhibited cell viability >50% (IC50). The study also encompassed evaluations of the structure and physical-chemical properties. The three-dimensional structures of AMPCry10Aa and AMPCry10Aa_5 were determined through nuclear magnetic resonance (NMR) spectroscopy, indicating the adoption of α-helical segments. Electron paramagnetic resonance (EPR) spectroscopy elucidated the mechanism of action, demonstrating that AMPCry10Aa_5 enters the outer membranes of E. coli and S. aureus , causing substantial increases in lipid fluidity, while AMPCry10Aa slightly increases lipid fluidity in E. coli . In conclusion, the results obtained underscore the potential of Cry10Aa as a source for developing antimicrobial peptides as alternatives to conventional antibiotics, offering a promising avenue in the battle against antibiotic resistance.
Competing Interests: The authors declare no competing financial interest.
(© 2024 The Authors. Published by American Chemical Society.)
References: Curr Med Chem. 2014;21(20):2299-321. (PMID: 24533812)
Int J Mol Sci. 2022 Dec 02;23(23):. (PMID: 36499519)
Crit Rev Clin Lab Sci. 2019 Sep;56(6):351-373. (PMID: 31397205)
Structure. 2020 Sep 1;28(9):1004-1013.e4. (PMID: 32470317)
Nat Protoc. 2008;3(2):163-75. (PMID: 18274517)
Proteins. 2005 Jun 1;59(4):687-96. (PMID: 15815974)
Chem Biol Interact. 2020 Apr 1;320:109023. (PMID: 32097615)
Bioinformatics. 2008 Sep 15;24(18):2101-2. (PMID: 18662927)
J Lab Physicians. 2010 Jul;2(2):78-81. (PMID: 21346901)
Methods Enzymol. 2001;339:71-90. (PMID: 11462826)
Am J Transl Res. 2019 Jul 15;11(7):3919-3931. (PMID: 31396309)
Toxins (Basel). 2014 Aug 13;6(8):2393-423. (PMID: 25123558)
Eur J Pharm Sci. 2020 Aug 1;151:105407. (PMID: 32504805)
Phytomedicine. 2016 Dec 15;23(14):1814-1820. (PMID: 27912884)
J Comput Chem. 2004 Oct;25(13):1605-12. (PMID: 15264254)
Clin Infect Dis. 2003 Jan 15;36(Suppl 1):S11-23. (PMID: 12516026)
Microb Pathog. 2016 Jun;95:32-42. (PMID: 26911646)
PLoS One. 2012;7(9):e45848. (PMID: 23029273)
J Magn Reson. 2010 Feb;202(2):223-33. (PMID: 20015671)
Methods Enzymol. 2022;663:67-98. (PMID: 35168798)
Nature. 2006 Oct 19;443(7113):867-9. (PMID: 17051220)
Front Immunol. 2012 Jul 31;3:221. (PMID: 23060873)
Nucleic Acids Res. 2004 Jul 1;32(Web Server issue):W665-7. (PMID: 15215472)
Acta Crystallogr D Biol Crystallogr. 1998 Sep 1;54(Pt 5):905-21. (PMID: 9757107)
Infect Dis (Lond). 2019 Sep;51(9):694-700. (PMID: 31355687)
PLoS One. 2012;7(12):e51444. (PMID: 23240023)
J Mol Graph Model. 2009 Jun-Jul;27(8):944-50. (PMID: 19285892)
Lett Appl Microbiol. 2023 Jan 23;76(1):. (PMID: 36688746)
Can J Microbiol. 2003 Jan;49(1):37-44. (PMID: 12674346)
Infect Dis (Lond). 2022 Jul;54(7):497-507. (PMID: 35277120)
Biochim Biophys Acta Gen Subj. 2018 Sep;1862(9):2043-2052. (PMID: 29928920)
Vet Dermatol. 2017 Feb;28(1):82-e19. (PMID: 27581211)
Biochim Biophys Acta Biomembr. 2021 Jan 1;1863(1):183473. (PMID: 32937102)
Angew Chem Int Ed Engl. 2008;47(31):5765-7. (PMID: 18576439)
Nat Rev Microbiol. 2015 Jan;13(1):42-51. (PMID: 25435309)
Biochim Biophys Acta. 2015 Nov;1848(11 Pt B):3047-54. (PMID: 25701232)
Biochim Biophys Acta. 2006 Sep;1758(9):1436-49. (PMID: 16678118)
J Org Chem. 2021 Nov 5;86(21):15242-15246. (PMID: 34641669)
Curr Top Microbiol Immunol. 2017;409:263-296. (PMID: 26659121)
Nature. 2021 Aug;596(7873):583-589. (PMID: 34265844)
Toxins (Basel). 2014 Dec 11;6(12):3296-325. (PMID: 25514092)
Proc Natl Acad Sci U S A. 1984 Jan;81(1):140-4. (PMID: 6582470)
J Biol Chem. 1990 Dec 25;265(36):22059-62. (PMID: 2266107)
Nucleic Acids Res. 2022 Jan 7;50(D1):D439-D444. (PMID: 34791371)
FEMS Microbiol Rev. 2016 Jan;40(1):133-59. (PMID: 25862689)
J Mater Chem B. 2022 May 18;10(19):3587-3600. (PMID: 35262120)
Protein Sci. 2018 Jan;27(1):112-128. (PMID: 28836357)
Biochim Biophys Acta. 2004 Nov 3;1666(1-2):40-50. (PMID: 15519307)
Biochim Biophys Acta. 2016 Nov;1858(11):2699-2708. (PMID: 27423268)
FEMS Microbiol Lett. 2001 Feb 5;195(1):1-8. (PMID: 11166987)
J Biol Chem. 2002 Sep 13;277(37):33913-21. (PMID: 12110678)
Proteins. 2003 Feb 15;50(3):496-506. (PMID: 12557191)
Nature. 1991 Oct 31;353(6347):815-21. (PMID: 1658659)
Biochemistry. 2008 Mar 25;47(12):3777-88. (PMID: 18307313)
Curr Pharm Des. 2010 May;16(15):1656-65. (PMID: 20222857)
Biochimie. 2019 Feb;157:10-21. (PMID: 30389515)
Nucleic Acids Res. 2009 Jul;37(Web Server issue):W510-4. (PMID: 19429685)
Methods Mol Biol. 2017;1548:35-49. (PMID: 28013495)
Acta Crystallogr D Biol Crystallogr. 2015 Jan 1;71(Pt 1):154-61. (PMID: 25615869)
Proc Natl Acad Sci U S A. 2001 Aug 28;98(18):10037-41. (PMID: 11517324)
Eur J Pharm Biopharm. 2015 May;92:65-73. (PMID: 25701808)
Nucleic Acids Res. 2007 Jul;35(Web Server issue):W407-10. (PMID: 17517781)
Med Res Rev. 2019 May;39(3):831-859. (PMID: 30353555)
J Biomol NMR. 1992 Nov;2(6):661-5. (PMID: 1490109)
Pharmacogn Rev. 2015 Jan-Jun;9(17):29-34. (PMID: 26009690)
Antimicrob Agents Chemother. 2007 Apr;51(4):1398-406. (PMID: 17158938)
EMBO J. 2022 Mar 1;41(5):e110737. (PMID: 35143047)
Biochim Biophys Acta Gen Subj. 2020 Sep;1864(9):129633. (PMID: 32416198)
Proteins. 1993 Dec;17(4):355-62. (PMID: 8108378)
Peptides. 2012 Dec;38(2):446-56. (PMID: 23022589)
Acc Chem Res. 2016 Jun 21;49(6):1130-8. (PMID: 27187572)
Int J Mol Sci. 2022 May 13;23(10):. (PMID: 35628272)
Annu Rev Microbiol. 2011;65:129-47. (PMID: 21639791)
Int J Antimicrob Agents. 2019 Oct;54(4):410-422. (PMID: 31404620)
Mol Membr Biol. 2002 Jan-Mar;19(1):1-10. (PMID: 11989818)
Nucleic Acids Res. 2005 Jul 1;33(Web Server issue):W89-93. (PMID: 15980588)
Pharmaceuticals (Basel). 2013 Nov 28;6(12):1543-75. (PMID: 24287494)
Biochim Biophys Acta. 2014 Jul;1838(7):1777-84. (PMID: 24657394)
Proteins. 2014 Sep;82(9):1960-70. (PMID: 24638929)
Free Radic Res. 2019 Jan;53(1):8-17. (PMID: 30403895)
Proc Natl Acad Sci U S A. 2012 May 22;109(21):8121-6. (PMID: 22566663)
Plant Physiol. 1995 Aug;108(4):1353-8. (PMID: 7659744)
Clin Infect Dis. 1999 May;28(5):1008-11. (PMID: 10452626)
Protein Sci. 2018 Jan;27(1):129-134. (PMID: 28875543)
J Chem Inf Model. 2010 Dec 27;50(12):2213-20. (PMID: 21090591)
Anaerobe. 2007 Feb;13(1):6-13. (PMID: 17126041)
Proc Natl Acad Sci U S A. 2004 Jan 27;101(4):959-63. (PMID: 14732697)
Biochim Biophys Acta Biomembr. 2018 Nov;1860(11):2404-2415. (PMID: 29902419)
Structure. 2008 Sep 10;16(9):1305-12. (PMID: 18786394)
Bioinformatics. 2011 Feb 1;27(3):343-50. (PMID: 21134891)
PLoS One. 2017 Jun 2;12(6):e0178943. (PMID: 28575099)
Antibiotics (Basel). 2022 Sep 21;11(10):. (PMID: 36289944)
Front Microbiol. 2020 Oct 16;11:582779. (PMID: 33178164)
Bioinformatics. 2007 Feb 1;23(3):381-2. (PMID: 17121777)
J Clin Microbiol. 2006 Feb;44(2):595-7. (PMID: 16455920)
J Mol Biol. 1988 Jun 5;201(3):601-19. (PMID: 3418712)
ACS Omega. 2018 May 31;3(5):5390-5398. (PMID: 30221230)
Amino Acids. 2012 Dec;43(6):2279-83. (PMID: 22555649)
تواريخ الأحداث: Date Created: 20240715 Latest Revision: 20240716
رمز التحديث: 20240716
مُعرف محوري في PubMed: PMC11238290
DOI: 10.1021/acsomega.3c07455
PMID: 39005792
قاعدة البيانات: MEDLINE
الوصف
تدمد:2470-1343
DOI:10.1021/acsomega.3c07455