التفاصيل البيبلوغرافية
| العنوان: |
Basement Membrane Matrix Encapsulated Cell Aggregation for Investigating Murine Spleen Tissue Formation. |
| المؤلفون: |
Tourle K; Clem Jones Centre for Regenerative Medicine, Faculty of Health Sciences and Medicine, Bond University; ktourle@bond.edu.au., Rucinski A; Clem Jones Centre for Regenerative Medicine, Faculty of Health Sciences and Medicine, Bond University., Grainger A; Clem Jones Centre for Regenerative Medicine, Faculty of Health Sciences and Medicine, Bond University., Limnios IJ; Clem Jones Centre for Regenerative Medicine, Faculty of Health Sciences and Medicine, Bond University., Gonzalez Ruiz M; Clem Jones Centre for Regenerative Medicine, Faculty of Health Sciences and Medicine, Bond University., Tan JKH; Clem Jones Centre for Regenerative Medicine, Faculty of Health Sciences and Medicine, Bond University. |
| المصدر: |
Journal of visualized experiments : JoVE [J Vis Exp] 2024 Jun 28 (208). Date of Electronic Publication: 2024 Jun 28. |
| نوع المنشور: |
Journal Article; Video-Audio Media |
| اللغة: |
English |
| بيانات الدورية: |
Publisher: MYJoVE Corporation Country of Publication: United States NLM ID: 101313252 Publication Model: Electronic Cited Medium: Internet ISSN: 1940-087X (Electronic) Linking ISSN: 1940087X NLM ISO Abbreviation: J Vis Exp Subsets: MEDLINE |
| أسماء مطبوعة: |
Original Publication: [Boston, Mass. : MYJoVE Corporation, 2006]- |
| مواضيع طبية MeSH: |
Spleen*/cytology , Basement Membrane*/cytology, Animals ; Mice ; Cell Aggregation/physiology |
| مستخلص: |
The spleen is an immune organ that plays a key role in blood-borne immune responses. The anatomical or functional loss of this tissue increases susceptibility to severe blood infections and sepsis. Auto-transplantation of spleen slices has been used clinically to replace lost tissue and restore immune function. However, the mechanism driving robust and immunologically functional spleen tissue regeneration has not been fully elucidated. Here, we aim to develop a method for aggregating and encapsulating spleen cells within a semi-solid matrix in order to investigate the cellular requirements for spleen tissue formation. Basement membrane matrix encapsulated cell constructs are amenable to both in vitro tissue culture of three-dimensional organoids as well as transplantation under the kidney capsule to directly assess in vivo tissue formation. By manipulating the input cells for aggregation and encapsulation, we demonstrate that graft-derived PDGFRβ + MAdCAM-1 - neonatal stromal cells are required for spleen tissue regeneration under animal transplantation models. |
| تواريخ الأحداث: |
Date Created: 20240715 Date Completed: 20240715 Latest Revision: 20240715 |
| رمز التحديث: |
20240715 |
| DOI: |
10.3791/66682 |
| PMID: |
39007618 |
| قاعدة البيانات: |
MEDLINE |
