دورية أكاديمية
أعرض في EDS

Evaluation of chimeric recombinant antigens for the serodiagnosis of Trypanosoma cruzi in dogs: a promising tool for Chagas disease surveillance.

التفاصيل البيبلوغرافية
العنوان: Evaluation of chimeric recombinant antigens for the serodiagnosis of Trypanosoma cruzi in dogs: a promising tool for Chagas disease surveillance.
المؤلفون: Fontes ND; Advanced Public Health Laboratory, Gonçalo Moniz Institute, Oswaldo Cruz Foundation, Salvador, Brazil., Habib FL; Advanced Public Health Laboratory, Gonçalo Moniz Institute, Oswaldo Cruz Foundation, Salvador, Brazil., Leony LM; Advanced Public Health Laboratory, Gonçalo Moniz Institute, Oswaldo Cruz Foundation, Salvador, Brazil.; Interdisciplinary Research Group in Biotechnology and Epidemiology of Infectious Diseases (GRUPIBE), Gonçalo Moniz Institute, Oswaldo Cruz Foundation (FIOCRUZ-BA), Salvador, Brazil., Freitas NEM; Advanced Public Health Laboratory, Gonçalo Moniz Institute, Oswaldo Cruz Foundation, Salvador, Brazil.; Interdisciplinary Research Group in Biotechnology and Epidemiology of Infectious Diseases (GRUPIBE), Gonçalo Moniz Institute, Oswaldo Cruz Foundation (FIOCRUZ-BA), Salvador, Brazil., Silva ÂAO; Advanced Public Health Laboratory, Gonçalo Moniz Institute, Oswaldo Cruz Foundation, Salvador, Brazil.; Interdisciplinary Research Group in Biotechnology and Epidemiology of Infectious Diseases (GRUPIBE), Gonçalo Moniz Institute, Oswaldo Cruz Foundation (FIOCRUZ-BA), Salvador, Brazil., Dantas-Torres F; Laboratory of Immunoparasitology, Department of Immunology, Aggeu Magalhães Institute, Oswaldo Cruz Foundation, Recife, Brazil., da Silva Sales KG; Laboratory of Immunoparasitology, Department of Immunology, Aggeu Magalhães Institute, Oswaldo Cruz Foundation, Recife, Brazil., da Câmara ACJ; Department of Clinical and Toxicological Analysis, Health Sciences Center, Federal University of Rio Grande do Norte, Natal, Brazil.; Postgraduate Program in Pharmaceutical Sciences, Federal University of Rio Grande do Norte, Natal, Brazil., de Araújo-Neto VT; Postgraduate Program in Pharmaceutical Sciences, Federal University of Rio Grande do Norte, Natal, Brazil., Amorim LDAF; Department of Statistics, Institute of Mathematics and Statistics, Federal University of Bahia, Salvador, Brazil., Celedon PAF; Molecular Biology of Trypanosomatids Laboratory, Carlos Chagas Institute, Oswaldo Cruz Foundation, Curitiba, Brazil., Zanchin NIT; Laboratory of Structural Biology & Protein Engineering, Carlos Chagas Institute, Oswaldo Cruz Foundation, Curitiba, Brazil.; Integrated Translational Program in Chagas disease from Fiocruz - Fio-Chagas, Rio de Janeiro, Brazil., Santos FLN; Advanced Public Health Laboratory, Gonçalo Moniz Institute, Oswaldo Cruz Foundation, Salvador, Brazil. fred.santos@fiocruz.br.; Interdisciplinary Research Group in Biotechnology and Epidemiology of Infectious Diseases (GRUPIBE), Gonçalo Moniz Institute, Oswaldo Cruz Foundation (FIOCRUZ-BA), Salvador, Brazil. fred.santos@fiocruz.br.; Integrated Translational Program in Chagas disease from Fiocruz - Fio-Chagas, Rio de Janeiro, Brazil. fred.santos@fiocruz.br.
المصدر: Parasites & vectors [Parasit Vectors] 2024 Jul 15; Vol. 17 (1), pp. 305. Date of Electronic Publication: 2024 Jul 15.
نوع المنشور: Journal Article; Evaluation Study
اللغة: English
بيانات الدورية: Publisher: BioMed Central Country of Publication: England NLM ID: 101462774 Publication Model: Electronic Cited Medium: Internet ISSN: 1756-3305 (Electronic) Linking ISSN: 17563305 NLM ISO Abbreviation: Parasit Vectors Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London : BioMed Central
مواضيع طبية MeSH: Chagas Disease*/diagnosis , Chagas Disease*/veterinary , Chagas Disease*/parasitology , Trypanosoma cruzi*/immunology , Trypanosoma cruzi*/genetics , Dog Diseases*/diagnosis , Dog Diseases*/parasitology , Antigens, Protozoan*/immunology , Antigens, Protozoan*/genetics , Serologic Tests*/methods , Serologic Tests*/veterinary , Enzyme-Linked Immunosorbent Assay*/methods , Enzyme-Linked Immunosorbent Assay*/veterinary , Sensitivity and Specificity* , Antibodies, Protozoan*/blood, Animals ; Dogs ; Recombinant Proteins/immunology ; Recombinant Proteins/genetics
مستخلص: Background: Chagas disease (CD), a neglected parasitic disease caused by Trypanosoma cruzi, poses a significant health threat in Latin America and has emerged globally because of human migration. Trypanosoma cruzi infects humans and over 100 other mammalian species, including dogs, which are important sentinels for assessing the risk of human infection. Nonetheless, the serodiagnosis of T. cruzi in dogs is still impaired by the absence of commercial tests. In this study, we investigated the diagnostic accuracy of four chimeric recombinant T. cruzi IBMP antigens (IBMP-8.1, IBMP-8.2, IBMP-8.3, and IBMP-8.4) for detecting anti-T. cruzi antibodies in dogs, using latent class analysis (LCA).
Methods: We examined 663 canine serum samples, employing indirect ELISA with the chimeric antigens. LCA was utilized to establish a latent variable as a gold standard for T. cruzi infection, revealing distinct response patterns for each antigen.
Results: The IBMP (Portuguese acronym for the Molecular Biology Institute of Paraná) antigens achieved area under the ROC curve (AUC) values ranging from 90.9% to 97.3%. The highest sensitivity was attributed to IBMP-8.2 (89.8%), while IBMP-8.1, IBMP-8.3, and IBMP-8.4 achieved 73.5%, 79.6%, and 85.7%, respectively. The highest specificity was observed for IBMP-8.4 (98.6%), followed by IBMP-8.2, IBMP-8.3, and IBMP-8.1 with specificities of 98.3%, 94.4%, and 92.7%, respectively. Predictive values varied according to prevalence, indicating higher effectiveness in endemic settings.
Conclusions: Our findings underscore the remarkable diagnostic performance of IBMP-8.2 and IBMP-8.4 for the serodiagnosis of Trypanosoma cruzi in dogs, representing a promising tool for the diagnosis of CD in dogs. These chimeric recombinant antigens may not only enhance CD surveillance strategies but also hold broader implications for public health, contributing to the global fight against this neglected tropical disease.
(© 2024. The Author(s).)
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معلومات مُعتمدة: BOL0298/2020 Foundation for Research Support of the State of Bahia (FAPESB); BOL0543/2020 Foundation for Research Support of the State of Bahia (FAPESB); 128257/2021-0 National Council for Scientific and Technological Development (CNPq); 304167/2019-3 National Council for Scientific and Technological Development (CNPq); 88887.805768/2023-00 Coordination of Superior Level Staff Improvement (CAPES); 88887.637758/2021-00 Coordination of Superior Level Staff Improvement (CAPES); Finance Code 001 Coordination of Superior Level Staff Improvement (CAPES); VPPCB-008-FIO-18-2-20 Inova Fiocruz/VPPCB; 306448/2023-8 Conselho Nacional de Desenvolvimento Científico e Tecnológico
فهرسة مساهمة: Keywords: Trypanosoma cruzi; Canine serodiagnosis; Chagas disease; Diagnostic performance; Latent class analysis; Recombinant chimeric antigens
المشرفين على المادة: 0 (Antigens, Protozoan)
0 (Antibodies, Protozoan)
0 (Recombinant Proteins)
تواريخ الأحداث: Date Created: 20240715 Date Completed: 20240716 Latest Revision: 20240718
رمز التحديث: 20240718
مُعرف محوري في PubMed: PMC11251128
DOI: 10.1186/s13071-024-06376-5
PMID: 39010122
قاعدة البيانات: MEDLINE
الوصف
تدمد:1756-3305
DOI:10.1186/s13071-024-06376-5