دورية أكاديمية
Study on the mechanism of mitigating radiation damage by improving the hematopoietic system and intestinal barrier with Tenebrio molitor peptides.
| العنوان: | Study on the mechanism of mitigating radiation damage by improving the hematopoietic system and intestinal barrier with Tenebrio molitor peptides. |
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| المؤلفون: | Shang Y; School of Food and Health, Beijing Technology and Business University, Beijing 100048, PR China. jiaymtd@163.com., Cui P; School of Food and Health, Beijing Technology and Business University, Beijing 100048, PR China. jiaymtd@163.com., Chen Y; School of Food and Health, Beijing Technology and Business University, Beijing 100048, PR China. jiaymtd@163.com., Zhang Z; School of Food and Health, Beijing Technology and Business University, Beijing 100048, PR China. jiaymtd@163.com., Li S; School of Food and Health, Beijing Technology and Business University, Beijing 100048, PR China. jiaymtd@163.com., Chen Z; School of Food and Health, Beijing Technology and Business University, Beijing 100048, PR China. jiaymtd@163.com., Ma A; School of Food and Health, Beijing Technology and Business University, Beijing 100048, PR China. jiaymtd@163.com., Jia Y; School of Food and Health, Beijing Technology and Business University, Beijing 100048, PR China. jiaymtd@163.com. |
| المصدر: | Food & function [Food Funct] 2024 Jul 29; Vol. 15 (15), pp. 8116-8127. Date of Electronic Publication: 2024 Jul 29. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Royal Society of Chemistry Country of Publication: England NLM ID: 101549033 Publication Model: Electronic Cited Medium: Internet ISSN: 2042-650X (Electronic) Linking ISSN: 20426496 NLM ISO Abbreviation: Food Funct Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Cambridge : Royal Society of Chemistry |
| مواضيع طبية MeSH: | Tenebrio* , Peptides*/pharmacology , Intestinal Mucosa*/metabolism , Intestinal Mucosa*/radiation effects , Intestinal Mucosa*/drug effects, Animals ; Mice ; Male ; Hematopoietic System/drug effects ; Hematopoietic System/radiation effects ; Radiation-Protective Agents/pharmacology ; Zonula Occludens-1 Protein/metabolism ; Zonula Occludens-1 Protein/genetics ; Gamma Rays/adverse effects ; Occludin/metabolism ; Occludin/genetics ; Intestines/drug effects ; Intestines/radiation effects |
| مستخلص: | Research on plant and animal peptides has garnered significant attention, but there is a lack of studies on the functional properties of Tenebrio molitor peptides, particularly in relation to their potential mitigating effect on radiation damage and the underlying mechanisms. This study aims to explore the protective effects of Tenebrio molitor peptides against radiation-induced damage. Mice were divided into five groups: normal, radiation model, and low-, medium-, and high-dose Tenebrio molitor peptide (TMP) groups (0.15 g per kg BW, 0.30 g per kg BW, and 0.60 g per kg BW). Various parameters such as blood cell counts, bone marrow DNA content, immune organ indices, serum levels of D-lactic acid, diamine oxidase (DAO), endotoxin (LPS), and inflammatory factors were assessed at 3 and 15 days post gamma irradiation. Additionally, the intestinal tissue morphology was examined through H&E staining, RT-qPCR experiments were conducted to analyze the expression of inflammatory factors in the intestine, and immunohistochemistry was utilized to evaluate the expression of tight junction proteins ZO-1 and Occludin in the intestine. The findings revealed that high-dose TMP significantly enhanced the hematopoietic system function in mice post radiation exposure, leading to increased spleen index, thymus index, blood cell counts, and bone marrow DNA production ( p < 0.05). Moreover, TMP improved the intestinal barrier integrity and reduced the intestinal permeability. Mechanistic insights suggested that these peptides may safeguard intestinal barrier function by downregulating the gene expression of inflammatory factors TNF-α, IL-1β, and IL-6, while upregulating the expression of tight junction proteins ZO-1 and Occludin ( p < 0.05). Overall, supplementation with TMP mitigates radiation-induced intestinal damage by enhancing the hematopoietic system and the intestinal barrier, offering valuable insights for further investigations into the mechanisms underlying the protective effects of these peptides against ionizing radiation. |
| المشرفين على المادة: | 0 (Peptides) 0 (Radiation-Protective Agents) 0 (Zonula Occludens-1 Protein) 0 (Occludin) |
| تواريخ الأحداث: | Date Created: 20240716 Date Completed: 20240729 Latest Revision: 20240729 |
| رمز التحديث: | 20240729 |
| DOI: | 10.1039/d4fo01141d |
| PMID: | 39011610 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 2042-650X |
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| DOI: | 10.1039/d4fo01141d |