دورية أكاديمية
The nuclear matrix protein HNRNPU restricts hepatitis B virus transcription by promoting OAS3-based activation of host innate immunity.
العنوان: | The nuclear matrix protein HNRNPU restricts hepatitis B virus transcription by promoting OAS3-based activation of host innate immunity. |
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المؤلفون: | Zeng Q; The Key Laboratory of Molecular Biology of Infectious Diseases designated by the Chinese Ministry of Education, Chongqing Medical University, Chongqing, China., Ren Y; The Key Laboratory of Molecular Biology of Infectious Diseases designated by the Chinese Ministry of Education, Chongqing Medical University, Chongqing, China., Wang Y; The Key Laboratory of Molecular Biology of Infectious Diseases designated by the Chinese Ministry of Education, Chongqing Medical University, Chongqing, China., Yang J; The Key Laboratory of Molecular Biology of Infectious Diseases designated by the Chinese Ministry of Education, Chongqing Medical University, Chongqing, China., Qin Y; The Key Laboratory of Molecular Biology of Infectious Diseases designated by the Chinese Ministry of Education, Chongqing Medical University, Chongqing, China., Yang L; The Key Laboratory of Molecular Biology of Infectious Diseases designated by the Chinese Ministry of Education, Chongqing Medical University, Chongqing, China., Zheng X; The Key Laboratory of Molecular Biology of Infectious Diseases designated by the Chinese Ministry of Education, Chongqing Medical University, Chongqing, China., Huang A; The Key Laboratory of Molecular Biology of Infectious Diseases designated by the Chinese Ministry of Education, Chongqing Medical University, Chongqing, China., Fan H; The Key Laboratory of Molecular Biology of Infectious Diseases designated by the Chinese Ministry of Education, Chongqing Medical University, Chongqing, China. |
المصدر: | Journal of medical virology [J Med Virol] 2024 Jul; Vol. 96 (7), pp. e29805. |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: Wiley-Liss Country of Publication: United States NLM ID: 7705876 Publication Model: Print Cited Medium: Internet ISSN: 1096-9071 (Electronic) Linking ISSN: 01466615 NLM ISO Abbreviation: J Med Virol Subsets: MEDLINE |
أسماء مطبوعة: | Publication: New York Ny : Wiley-Liss Original Publication: New York, Liss. |
مواضيع طبية MeSH: | Immunity, Innate* , Hepatitis B virus*/immunology , Hepatitis B virus*/genetics , 2',5'-Oligoadenylate Synthetase*/genetics , 2',5'-Oligoadenylate Synthetase*/metabolism , Transcription, Genetic*, Humans ; Host-Pathogen Interactions/immunology ; Host-Pathogen Interactions/genetics ; Hep G2 Cells ; Hepatitis B/immunology ; Hepatitis B/virology ; Hepatitis B/genetics ; Viral Regulatory and Accessory Proteins/genetics ; Viral Regulatory and Accessory Proteins/metabolism ; Viral Regulatory and Accessory Proteins/immunology ; Trans-Activators |
مستخلص: | Heterogeneous nuclear protein U (HNRNPU) plays a pivotal role in innate immunity by facilitating chromatin opening to activate immune genes during host defense against viral infection. However, the mechanism by which HNRNPU is involved in Hepatitis B virus (HBV) transcription regulation through mediating antiviral immunity remains unknown. Our study revealed a significant decrease in HNRNPU levels during HBV transcription, which depends on HBx-DDB1-mediated degradation. Overexpression of HNRNPU suppressed HBV transcription, while its knockdown effectively promoted viral transcription, indicating HNRNPU as a novel host restriction factor for HBV transcription. Mechanistically, HNRNPU inhibits HBV transcription by activating innate immunity through primarily the positive regulation of the interferon-stimulating factor 2'-5'-oligoadenylate synthetase 3, which mediates an ribonuclease L-dependent mechanism to enhance innate immune responses. This study offers new insights into the host immune regulation of HBV transcription and proposes potential targets for therapeutic intervention against HBV infection. (© 2024 The Author(s). Journal of Medical Virology published by Wiley Periodicals LLC.) |
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معلومات مُعتمدة: | 2022YFA1303600 National Key Research and Development Program of China; D20028 111 Project; Chongqing Medical University |
فهرسة مساهمة: | Keywords: HBV transcription; HNRNPU; OAS3; RNase L; innate immunity |
المشرفين على المادة: | EC 2.7.7.84 (2',5'-Oligoadenylate Synthetase) EC 2.7.7.84 (OAS3 protein, human) 0 (Viral Regulatory and Accessory Proteins) 0 (hepatitis B virus X protein) 0 (Trans-Activators) |
تواريخ الأحداث: | Date Created: 20240716 Date Completed: 20240716 Latest Revision: 20240814 |
رمز التحديث: | 20240814 |
DOI: | 10.1002/jmv.29805 |
PMID: | 39011773 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1096-9071 |
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DOI: | 10.1002/jmv.29805 |