دورية أكاديمية
أعرض في EDS

A cyclic di-GMP-binding adaptor protein interacts with a N5-glutamine methyltransferase to regulate the pathogenesis in Xanthomonas citri subsp. citri.

التفاصيل البيبلوغرافية
العنوان: A cyclic di-GMP-binding adaptor protein interacts with a N5-glutamine methyltransferase to regulate the pathogenesis in Xanthomonas citri subsp. citri.
المؤلفون: Shi Y; Integrative Microbiology Research Centre, Guangdong Province Key Laboratory of Microbial Signals and Disease Control, South China Agricultural University, Guangzhou, China.; Guangdong Provincial Key Laboratory of Utilization and Conservation of Food and Medicinal Resources in Northern Region, Shaoguan University, Shaoguan, China., Cheng T; Integrative Microbiology Research Centre, Guangdong Province Key Laboratory of Microbial Signals and Disease Control, South China Agricultural University, Guangzhou, China., Cheang QW; School of Biological Sciences, Nanyang Technological University, Singapore, Singapore., Zhao X; Integrative Microbiology Research Centre, Guangdong Province Key Laboratory of Microbial Signals and Disease Control, South China Agricultural University, Guangzhou, China., Xu Z; Integrative Microbiology Research Centre, Guangdong Province Key Laboratory of Microbial Signals and Disease Control, South China Agricultural University, Guangzhou, China., Liang ZX; School of Biological Sciences, Nanyang Technological University, Singapore, Singapore., Xu L; Integrative Microbiology Research Centre, Guangdong Province Key Laboratory of Microbial Signals and Disease Control, South China Agricultural University, Guangzhou, China., Wang J; Integrative Microbiology Research Centre, Guangdong Province Key Laboratory of Microbial Signals and Disease Control, South China Agricultural University, Guangzhou, China.
المصدر: Molecular plant pathology [Mol Plant Pathol] 2024 Jul; Vol. 25 (7), pp. e13496.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Blackwell Science in collaboration with the British Society of Plant Pathology Country of Publication: England NLM ID: 100954969 Publication Model: Print Cited Medium: Internet ISSN: 1364-3703 (Electronic) Linking ISSN: 13643703 NLM ISO Abbreviation: Mol Plant Pathol Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Oxford, UK : Blackwell Science in collaboration with the British Society of Plant Pathology, c2000-
مواضيع طبية MeSH: Xanthomonas*/pathogenicity , Xanthomonas*/metabolism , Xanthomonas*/genetics , Cyclic GMP*/metabolism , Cyclic GMP*/analogs & derivatives , Bacterial Proteins*/metabolism , Bacterial Proteins*/genetics , Methyltransferases*/metabolism , Methyltransferases*/genetics, Virulence ; Plant Diseases/microbiology ; Protein Binding
مستخلص: The second messenger cyclic diguanylate monophosphate (c-di-GMP) regulates a wide range of bacterial behaviours through diverse mechanisms and binding receptors. Single-domain PilZ proteins, the most widespread and abundant known c-di-GMP receptors in bacteria, act as trans-acting adaptor proteins that enable c-di-GMP to control signalling pathways with high specificity. This study identifies a single-domain PilZ protein, XAC3402 (renamed N5MapZ), from the phytopathogen Xanthomonas citri subsp. citri (Xcc), which modulates Xcc virulence by directly interacting with the methyltransferase HemK. Through yeast two-hybrid, co-immunoprecipitation and immunofluorescent staining, we demonstrated that N5MapZ and HemK interact directly under both in vitro and in vivo conditions, with the strength of the protein-protein interaction decreasing at high c-di-GMP concentrations. This finding distinguishes N5MapZ from other characterized single-domain PilZ proteins, as it was previously known that c-di-GMP enhances the interaction between those single-domain PilZs and their protein partners. This observation is further supported by the fact that the c-di-GMP binding-defective mutant N5MapZ R10A can interact with HemK to inhibit the methylation of the class 1 translation termination release factor PrfA. Additionally, we found that HemK plays an important role in Xcc pathogenesis, as the deletion of hemK leads to extensive phenotypic changes, including reduced virulence in citrus plants, decreased motility, production of extracellular enzymes and stress tolerance. Gene expression analysis has revealed that c-di-GMP and the HemK-mediated pathway regulate the expression of multiple virulence effector proteins, uncovering a novel regulatory mechanism through which c-di-GMP regulates Xcc virulence by mediating PrfA methylation via the single-domain PilZ adaptor protein N5MapZ.
(© 2024 The Author(s). Molecular Plant Pathology published by British Society for Plant Pathology and John Wiley & Sons Ltd.)
References: BMC Microbiol. 2012 Mar 09;12:31. (PMID: 22404966)
Mol Plant Pathol. 2012 Oct;13(8):865-76. (PMID: 22435635)
Mol Microbiol. 2006 May;60(4):1026-43. (PMID: 16677312)
Biochem Soc Trans. 2019 Feb 28;47(1):381-388. (PMID: 30710060)
J Biol Chem. 2016 Jul 29;291(31):16112-23. (PMID: 27231351)
Microbiol Res. 2015 Jan;170:177-83. (PMID: 25126992)
Trends Microbiol. 2021 Feb;29(2):182-183. (PMID: 32616307)
PLoS Pathog. 2014 Feb 27;10(2):e1003945. (PMID: 24586158)
Annu Rev Genet. 2022 Nov 30;56:187-205. (PMID: 36055649)
Appl Microbiol Biotechnol. 2010 Jun;87(2):467-77. (PMID: 20449739)
J Bacteriol. 2012 Nov;194(21):5922-31. (PMID: 22942245)
Mol Plant Microbe Interact. 2014 Jun;27(6):578-89. (PMID: 24548063)
J Biol Chem. 2024 Mar;300(3):105741. (PMID: 38340793)
J Mol Biol. 2009 Nov 6;393(4):848-66. (PMID: 19646999)
FEMS Microbiol Rev. 2020 Jan 1;44(1):1-32. (PMID: 31578554)
Mol Microbiol. 2012 Dec;86(6):1424-40. (PMID: 23163901)
Mol Plant Pathol. 2012 Dec;13(9):1047-59. (PMID: 22788999)
Environ Microbiol. 2019 Dec;21(12):4504-4520. (PMID: 31301270)
J Biol Chem. 2006 Oct 13;281(41):30310-4. (PMID: 16920715)
Mol Plant Pathol. 2022 Aug;23(8):1187-1199. (PMID: 35460168)
Nucleic Acids Res. 2009 Jan;37(Database issue):D211-5. (PMID: 18940856)
Mol Plant Microbe Interact. 2015 Feb;28(2):143-53. (PMID: 25338144)
mBio. 2022 Aug 30;13(4):e0167122. (PMID: 35916402)
Proc Natl Acad Sci U S A. 2013 Oct 29;110(44):17856-61. (PMID: 24127606)
Mol Microbiol. 2009 Mar;71(6):1464-76. (PMID: 19220743)
Appl Environ Microbiol. 2015 Jul;81(13):4358-67. (PMID: 25911481)
Mol Cell. 2020 Feb 6;77(3):443-445. (PMID: 32032510)
J Mol Biol. 2013 Jun 26;425(12):2174-97. (PMID: 23507310)
Annu Rev Microbiol. 2019 Sep 8;73:387-406. (PMID: 31500536)
Cell Host Microbe. 2020 Apr 8;27(4):601-613.e7. (PMID: 32272078)
Cell. 2010 Apr 2;141(1):107-16. (PMID: 20303158)
J Biol Chem. 2005 Jan 28;280(4):2439-45. (PMID: 15509572)
mBio. 2020 Dec 1;11(6):. (PMID: 33262261)
Nat Rev Microbiol. 2017 May;15(5):271-284. (PMID: 28163311)
Mol Plant Microbe Interact. 2011 Jun;24(6):649-61. (PMID: 21261465)
Mol Plant Microbe Interact. 2020 Oct;33(10):1196-1208. (PMID: 32720873)
Proc Natl Acad Sci U S A. 2022 Oct 11;119(41):e2209838119. (PMID: 36191190)
Mol Microbiol. 2010 Jun 1;76(5):1295-305. (PMID: 20444091)
Mol Plant Microbe Interact. 2012 Jan;25(1):69-84. (PMID: 21899385)
Mol Cell. 2010 Apr 9;38(1):128-39. (PMID: 20346719)
Proc Natl Acad Sci U S A. 2017 Dec 5;114(49):13006-13011. (PMID: 29158389)
J Bacteriol. 2013 Feb;195(3):417-28. (PMID: 23161026)
Methods. 2001 Dec;25(4):402-8. (PMID: 11846609)
Sci Signal. 2016 Oct 18;9(450):ra102. (PMID: 27811183)
J Bacteriol. 2011 Apr;193(7):1590-9. (PMID: 21257774)
Int J Mol Sci. 2022 Apr 01;23(7):. (PMID: 35409293)
Nat Rev Microbiol. 2020 Aug;18(8):415-427. (PMID: 32346148)
Mol Plant Pathol. 2008 Nov;9(6):819-24. (PMID: 19019010)
FEMS Microbiol Rev. 2010 Mar;34(2):107-33. (PMID: 19925633)
Mol Biol (Mosk). 2022 May-Jun;56(3):439-450. (PMID: 35621100)
Biotechnol Lett. 2009 Feb;31(2):265-70. (PMID: 18854951)
PLoS Pathog. 2019 Aug 13;15(8):e1007952. (PMID: 31408509)
PLoS Pathog. 2014 Oct 16;10(10):e1004429. (PMID: 25329577)
Genet Mol Biol. 2010 Jan;33(1):146-53. (PMID: 21637619)
Mol Plant Pathol. 2022 Jun;23(6):870-884. (PMID: 35254732)
Gene. 1994 Jul 22;145(1):69-73. (PMID: 8045426)
معلومات مُعتمدة: 2021A1515010761 The Natural Science Foundation of Guangdong Province, China; 2022A1515010869 The Natural Science Foundation of Guangdong Province, China; 202201010058 The Basic and Applied basic Research Project of Guangzhou basic Research Program, China
فهرسة مساهمة: Keywords: HemK; PrfA; Xanthomonas citri subsp. citri; cyclic di‐GMP; exoenzyme; motility; stress tolerance; virulence
المشرفين على المادة: H2D2X058MU (Cyclic GMP)
61093-23-0 (bis(3',5')-cyclic diguanylic acid)
0 (Bacterial Proteins)
EC 2.1.1.- (Methyltransferases)
SCR Organism: Xanthomonas citri
تواريخ الأحداث: Date Created: 20240716 Date Completed: 20240716 Latest Revision: 20240718
رمز التحديث: 20240718
مُعرف محوري في PubMed: PMC11250160
DOI: 10.1111/mpp.13496
PMID: 39011828
قاعدة البيانات: MEDLINE
الوصف
تدمد:1364-3703
DOI:10.1111/mpp.13496