التفاصيل البيبلوغرافية
| العنوان: |
Physical cell-cell contact elicits specific transcriptomic responses in wine yeast species. |
| المؤلفون: |
Luyt NA; Department of Viticulture and Oenology, South African Grape and Wine Research Institute, Stellenbosch University, Stellenbosch, Western Cape, South Africa., de Witt RN; Centre for Bioinformatics and Computational Biology, Stellenbosch University, Stellenbosch, Western Cape, South Africa., Divol B; Department of Viticulture and Oenology, South African Grape and Wine Research Institute, Stellenbosch University, Stellenbosch, Western Cape, South Africa., Patterton HG; Centre for Bioinformatics and Computational Biology, Stellenbosch University, Stellenbosch, Western Cape, South Africa., Setati ME; Department of Viticulture and Oenology, South African Grape and Wine Research Institute, Stellenbosch University, Stellenbosch, Western Cape, South Africa., Taillandier P; Institut National Polytechnique de Toulouse, Paul Sabatier Université, Laboratoire de Génie Chimique, Université de Toulouse, Toulouse, France., Bauer FF; Department of Viticulture and Oenology, South African Grape and Wine Research Institute, Stellenbosch University, Stellenbosch, Western Cape, South Africa. |
| المصدر: |
Microbiology spectrum [Microbiol Spectr] 2024 Jul 16, pp. e0057223. Date of Electronic Publication: 2024 Jul 16. |
| Publication Model: |
Ahead of Print |
| نوع المنشور: |
Journal Article |
| اللغة: |
English |
| بيانات الدورية: |
Publisher: ASM Press Country of Publication: United States NLM ID: 101634614 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2165-0497 (Electronic) Linking ISSN: 21650497 NLM ISO Abbreviation: Microbiol Spectr Subsets: MEDLINE |
| أسماء مطبوعة: |
Original Publication: Washington, DC : ASM Press, 2013- |
| مستخلص: |
Fermenting grape juice provides a habitat for a well-mapped and evolutionarily relevant microbial ecosystem consisting of many natural or inoculated strains of yeasts and bacteria. The molecular nature of many of the ecological interactions within this ecosystem remains poorly understood, with the partial exception of interactions of a metabolic nature such as competition for nutrients and production of toxic metabolites/peptides. Data suggest that physical contact between species plays a significant role in the phenotypic outcome of interspecies interactions. However, the molecular nature of the mechanisms regulating these phenotypes remains unknown. Here, we present a transcriptomic analysis of physical versus metabolic contact between two wine relevant yeast species, Saccharomyces cerevisiae and Lachancea thermotolerans . The data show that these species respond to the physical presence of the other species. In S. cerevisiae , physical contact results in the upregulation of genes involved in maintaining cell wall integrity, cell wall structural components, and genes involved in the production of H 2 S. In L. thermotolerans , HSP stress response genes were the most significantly upregulated gene family. Both yeasts downregulated genes belonging to the FLO family, some of which play prominent roles in cellular adhesion. qPCR analysis indicates that the expression of some of these genes is regulated in a species-specific manner, suggesting that yeasts adjust gene expression to specific biotic challenges or interspecies interactions. These findings provide fundamental insights into yeast interactions and evolutionary adaptations of these species to the wine ecosystem.IMPORTANCEWithin the wine ecosystem, yeasts are the most relevant contributors to alcoholic fermentation and wine organoleptic characteristics. While some studies have described yeast-yeast interactions during alcoholic fermentation, such interactions remain ill-defined, and little is understood regarding the molecular mechanisms behind many of the phenotypes observed when two or more species are co-cultured. In particular, no study has investigated transcriptional regulation in response to physical interspecies cell-cell contact, as opposed to the generally better understood/characterized metabolic interactions. These data are of direct relevance to our understanding of microbial ecological interactions in general while also creating opportunities to improve ecosystem-based biotechnological applications such as wine fermentation. Furthermore, the presence of competitor species has rarely been considered an evolutionary biotic selection pressure. In this context, the data reveal novel gene functions. This, and further such analysis, is likely to significantly enlarge the genome annotation space. |
| فهرسة مساهمة: |
Keywords: RNA-seq; cell-cell interactions; microbial ecology; wine ecosystem; yeast evolutionary adaptations; yeast transcriptomic responses |
| تواريخ الأحداث: |
Date Created: 20240716 Latest Revision: 20240716 |
| رمز التحديث: |
20240716 |
| DOI: |
10.1128/spectrum.00572-23 |
| PMID: |
39012115 |
| قاعدة البيانات: |
MEDLINE |
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