دورية أكاديمية
أعرض في EDS

RGS1 and CREB5 are direct and common transcriptional targets of ZNF384-fusion proteins.

التفاصيل البيبلوغرافية
العنوان: RGS1 and CREB5 are direct and common transcriptional targets of ZNF384-fusion proteins.
المؤلفون: Yamada C; Division of Cellular and Genetic Sciences, Department of Integrated Health Sciences, Nagoya University Graduate School of Medicine, Nagoya, Japan., Okada K; Division of Cellular and Genetic Sciences, Department of Integrated Health Sciences, Nagoya University Graduate School of Medicine, Nagoya, Japan., Odaira K; Division of Cellular and Genetic Sciences, Department of Integrated Health Sciences, Nagoya University Graduate School of Medicine, Nagoya, Japan., Tokoro M; Division of Cellular and Genetic Sciences, Department of Integrated Health Sciences, Nagoya University Graduate School of Medicine, Nagoya, Japan., Iwamoto E; Clinical Research Center, National Hospital Organization Nagoya Medical Center, Nagoya, Japan., Sanada M; Clinical Research Center, National Hospital Organization Nagoya Medical Center, Nagoya, Japan., Noura M; Division of Cellular and Genetic Sciences, Department of Integrated Health Sciences, Nagoya University Graduate School of Medicine, Nagoya, Japan., Okamoto S; Division of Cellular and Genetic Sciences, Department of Integrated Health Sciences, Nagoya University Graduate School of Medicine, Nagoya, Japan., Yasuda T; Clinical Research Center, National Hospital Organization Nagoya Medical Center, Nagoya, Japan., Tsuzuki S; Department of Biochemistry, Aichi Medical University School of Medicine, Nagakute, Japan., Kiyoi H; Department of Hematology and Oncology, Nagoya University Graduate School of Medicine, Nagoya, Japan., Hayakawa F; Division of Cellular and Genetic Sciences, Department of Integrated Health Sciences, Nagoya University Graduate School of Medicine, Nagoya, Japan.
المصدر: Cancer medicine [Cancer Med] 2024 Jul; Vol. 13 (14), pp. e7471.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: John Wiley & Sons Ltd Country of Publication: United States NLM ID: 101595310 Publication Model: Print Cited Medium: Internet ISSN: 2045-7634 (Electronic) Linking ISSN: 20457634 NLM ISO Abbreviation: Cancer Med Subsets: MEDLINE
أسماء مطبوعة: Original Publication: [Malden, MA] : John Wiley & Sons Ltd., c2012-
مواضيع طبية MeSH: Cyclic AMP Response Element-Binding Protein*/metabolism , Cyclic AMP Response Element-Binding Protein*/genetics , Oncogene Proteins, Fusion*/genetics , Oncogene Proteins, Fusion*/metabolism , RGS Proteins*/genetics , RGS Proteins*/metabolism, Humans ; Cell Line, Tumor ; Chemokine CXCL12/genetics ; Chemokine CXCL12/metabolism ; Gene Expression Regulation, Leukemic ; Receptors, CXCR4/genetics ; Receptors, CXCR4/metabolism ; Trans-Activators
مستخلص: Background: ZNF384-fusion (Z-fusion) genes were recently identified in B-cell acute lymphoblastic leukemia (B-ALL) and are frequent in Japanese adult patients. The frequency is about 20% in those with Philadelphia chromosome-negative B-ALL. ZNF384 is a transcription factor and Z-fusion proteins have increased transcriptional activity; however, the detailed mechanisms of leukemogenesis of Z-fusion proteins have yet to be clarified.
Methods: We established three transfectants of cell lines expressing different types of Z-fusion proteins, and analyzed their gene expression profile (GEP) by RNA-seq. We also analyzed the GEP of clinical ALL samples using our previous RNA-seq data of 323 Japanese ALL patients. We selected upregulated genes in both Z-fusion gene-expressing transfectants and Z-fusion gene-positive ALL samples, and investigated the binding of Z-fusion proteins to regulatory regions of the candidate genes by ChIP-qPCR.
Results: We selected six commonly upregulated genes. After the investigation by ChIP-qPCR, we finally identified CREB5 and RGS1 as direct and common target genes. RGS1 is an inhibitor of CXCL12-CXCR4 signaling that is required for the homing of hematopoietic progenitor cells to the bone marrow microenvironment and development of B cells. Consistent with this, Z-fusion gene transfectants showed impaired migration toward CXCL12.
Conclusions: We identified CREB5 and RGS1 as direct and common transcriptional targets of Z-fusion proteins. The present results provide novel insight into the aberrant transcriptional regulation by Z-fusion proteins.
(© 2024 The Author(s). Cancer Medicine published by John Wiley & Sons Ltd.)
References: Br J Haematol. 2007 May;137(4):288-96. (PMID: 17456052)
Oncol Lett. 2017 Dec;14(6):8156-8161. (PMID: 29250192)
J Bone Miner Res. 2001 Jan;16(1):10-23. (PMID: 11149472)
J Exp Clin Cancer Res. 2020 Aug 25;39(1):168. (PMID: 32843066)
Proc Natl Acad Sci U S A. 2018 Dec 11;115(50):E11711-E11720. (PMID: 30487223)
Blood Cancer Discov. 2022 May 5;3(3):240-263. (PMID: 35247902)
Haematologica. 2017 Jan;102(1):118-129. (PMID: 27634205)
Cancer Med. 2024 Jul;13(14):e7471. (PMID: 39015025)
Leuk Lymphoma. 2023 Feb;64(2):483-486. (PMID: 36533589)
Science. 2004 Oct 22;306(5696):636-40. (PMID: 15499007)
J Transl Med. 2022 Jul 25;20(1):334. (PMID: 35879796)
Blood. 2022 Mar 24;139(12):1850-1862. (PMID: 34695176)
J Hematol Oncol. 2015 Aug 21;8:100. (PMID: 26293203)
Front Oncol. 2021 Mar 17;11:632532. (PMID: 33816270)
Blood. 2008 Feb 1;111(3):1182-92. (PMID: 17975014)
Blood. 2011 Jan 13;117(2):429-39. (PMID: 20833981)
Int J Hematol. 2017 Aug;106(2):269-281. (PMID: 28378055)
J Immunol. 2005 Mar 1;174(5):2582-90. (PMID: 15728464)
J Exp Med. 2005 Mar 21;201(6):961-70. (PMID: 15781586)
Mol Cell Biol. 2000 Mar;20(5):1649-58. (PMID: 10669742)
Nat Genet. 2016 May;48(5):569-74. (PMID: 27019113)
FEBS Lett. 2019 Aug;593(16):2151-2161. (PMID: 31234226)
Genome Res. 2017 Feb;27(2):185-195. (PMID: 27903646)
Genes Cells. 2004 Jun;9(6):575-89. (PMID: 15189450)
Biochem Biophys Res Commun. 2006 Jul 14;345(4):1471-80. (PMID: 16730330)
J Biol Chem. 2019 Dec 6;294(49):18571-18585. (PMID: 31636120)
Nat Genet. 2019 Feb;51(2):296-307. (PMID: 30643249)
Mol Med. 2022 Sep 13;28(1):111. (PMID: 36100877)
EBioMedicine. 2016 Jun;8:173-183. (PMID: 27428428)
Cell Death Dis. 2019 Jun 5;10(6):444. (PMID: 31168049)
Cancer Sci. 2023 Mar;114(3):781-792. (PMID: 36341510)
Nat Commun. 2022 Sep 14;13(1):5401. (PMID: 36104354)
Sci Rep. 2016 May 03;6:25389. (PMID: 27138288)
Int J Clin Exp Pathol. 2018 Oct 01;11(10):4908-4916. (PMID: 31949566)
Cell Cycle. 2005 Sep;4(9):1134-5. (PMID: 16096372)
معلومات مُعتمدة: 18H02835 Japan Society for the Promotion of Science; 21 K19505 Japan Society for the Promotion of Science; 22H03102 Japan Society for the Promotion of Science; JP 23ck0106851 Japan Agency for Medical Research and Development; JP19ck0106331 Japan Agency for Medical Research and Development; JP22ck0106607 Japan Agency for Medical Research and Development
فهرسة مساهمة: Keywords: ZNF384; RNA‐seq; acute lymphoblastic leukemia; fusion gene; transcriptional target
المشرفين على المادة: 0 (Chemokine CXCL12)
0 (CXCL12 protein, human)
0 (CXCR4 protein, human)
0 (Cyclic AMP Response Element-Binding Protein)
0 (Oncogene Proteins, Fusion)
0 (Receptors, CXCR4)
0 (RGS Proteins)
0 (RGS1 protein, human)
0 (Trans-Activators)
0 (ZNF384 protein, human)
0 (CREB5 protein, human)
تواريخ الأحداث: Date Created: 20240717 Date Completed: 20240717 Latest Revision: 20240722
رمز التحديث: 20240722
مُعرف محوري في PubMed: PMC11252495
DOI: 10.1002/cam4.7471
PMID: 39015025
قاعدة البيانات: MEDLINE
الوصف
تدمد:2045-7634
DOI:10.1002/cam4.7471