دورية أكاديمية
Metabolic disruptions in Biomphalaria glabrata induced by Heterorhabditis bacteriophora HP88: implications for entomopathogenic nematodes in biological control.
| العنوان: | Metabolic disruptions in Biomphalaria glabrata induced by Heterorhabditis bacteriophora HP88: implications for entomopathogenic nematodes in biological control. |
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| المؤلفون: | Tunholi VM; Departamento de Microbiologia, Imunologia, Parasitologia e Patologia da Universidade Federal de Goiás, Setor Universitário, Goiânia, GO, Brazil. Electronic address: victortunholi@gmail.com., Amaral LS; Departamento de Medicina Veterinária, Centro de Ciências Agrárias e Engenharias, Universidade Federal do Espírito Santo (UFES), Alegre, ES, Brazil. Electronic address: duarte@ensp.fiocruz.br., Sperandio NDC; Departamento de Medicina Veterinária, Centro de Ciências Agrárias e Engenharias, Universidade Federal do Espírito Santo (UFES), Alegre, ES, Brazil. Electronic address: nataniasperandio@gmail.com., Altoé LSC; Grupo de Biologia Computacional e de Sistemas, Núcleo de Genética Humana e Molecular, Universidade Federal do Espírito Santo (UFES), Vitória, ES, Brazil. Electronic address: lorenascast@gmail.com., Tunholi-Alves VM; Universidade Estácio de Sá. Bolsista do Programa Pesquisa e Produtividade da (UNESA), Rio de Janeiro, RJ, Brazil. Electronic address: vinicius_menezestunholi@yahoo.com.br., Garcia J; Laboratório de Biologia e Parasitologia de Mamíferos Silvestres Reservatórios, Instituto Oswaldo Cruz, Fiocruz, Av. Brasil, 4365 -Manguinhos, Rio de Janeiro, RJ 21040-900, Brazil. Electronic address: juberlan@ioc.fiocruz.br., Mota EM; Laboratório de Patologia, Instituto Oswaldo Cruz, Fiocruz, Av. Brasil 4365, Rio de Janeiro 21040-30, Brazil. Electronic address: ester@ioc.fiocruz.br., Couto-Chambarelli MCMD; Curso de Pós-Graduação em Ciências, Departamento de Parasitologia Animal (DPA), Instituto de Veterinária, Universidade Federal Rural do Rio de Janeiro (UFRRJ), Rio de Janeiro, Brasil. Electronic address: melcmcouto@yahoo.com.br., Monteiro CMO; Departamento de Microbiologia, Imunologia, Parasitologia e Patologia da Universidade Federal de Goiás, Setor Universitário, Goiânia, GO, Brazil. Electronic address: caiosat@gmail.com., Martins IVF; Departamento de Medicina Veterinária, Centro de Ciências Agrárias e Engenharias, Universidade Federal do Espírito Santo (UFES), Alegre, ES, Brazil. Electronic address: ivfmartins@gmail.com. |
| المصدر: | Experimental parasitology [Exp Parasitol] 2024 Jul 15, pp. 108804. Date of Electronic Publication: 2024 Jul 15. |
| Publication Model: | Ahead of Print |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Academic Press Country of Publication: United States NLM ID: 0370713 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1090-2449 (Electronic) Linking ISSN: 00144894 NLM ISO Abbreviation: Exp Parasitol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Orlando, FL : Academic Press Original Publication: New York. |
| مستخلص: | Research on the use of entomopathogenic nematodes (EPNs) as a potential tool for the biological control of invertebrates has been growing in recent years, including studies involving snails with One Health importance. In this study, the effect of exposure time (24 or 48 hours) of Heterorhabditis bacteriophora HP88 on the activities of alanine aminotransferase (ALT) and aspartate aminotransferase (AST), as well as the concentration of total proteins, uric acid, and urea in the hemolymph of Biomphalaria glabrata, were investigated. The concentrations of these metabolic markers were measured weekly until the end of the third week after exposure. Along with a significant reduction in total protein levels, a significant increase (p<0.01) in uric acid and urea contents in the hemolymph of B. glabrata exposed to H. bacteriophora was observed. The accumulation of urea in these mollusks could lead to deleterious effects due to its high toxicity, inducing significant cell damage. Variations in transaminase activities were also observed, with snails exposed to EPNs showing significantly higher values (p<0.01) than individuals in the control group, both for ALT and AST. These results indicate that experimental exposure to infective juveniles of H. bacteriophora causes significant alterations in the metabolic pattern of B. glabrata, compromising the maintenance of its homeostasis. Finally, exposure for 48 hours caused more damage to the planorbid in question compared to snails exposed for 24 hours, suggesting that the exposure time may influence the intensity of the host's response. Competing Interests: Declaration of Competing Interest ☒ The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. ☐ The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: (Copyright © 2024. Published by Elsevier Inc.) |
| فهرسة مساهمة: | Keywords: Biomphalaria glabrata; Entomopathogenic nematodes (EPNs); Parasite-host relationship; Schistosoma mansoni |
| تواريخ الأحداث: | Date Created: 20240717 Latest Revision: 20240717 |
| رمز التحديث: | 20240718 |
| DOI: | 10.1016/j.exppara.2024.108804 |
| PMID: | 39019304 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1090-2449 |
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| DOI: | 10.1016/j.exppara.2024.108804 |